Responses of basophilic cells of the digestive gland of mussels to petroleum hydrocarbon exposure

Cajaraville, M.P.; Díez, G.; Marigómez, Ionan; Angulo, E.

doi:10.3354/dao009221

Cited by 79 publications

(30 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The application of histochemical, cytological, and cell physiological parameters as biomarkers of toxicant exposure in gastropods might be important for monitoring purposes in environmental toxicology (Cajaraville et al 1990;Dallinger et al 2001a;Zaldibar et al 2007a). For the slug D. reticulatum exposed to moderate or medium Cd concentrations in their feed, for example, a status of "reaction" (compensation) has been assessed in midgut gland cells characterized, among others, by the vesiculation and dilation of ER, with progressive intensification of these alterations at higher Cd concentrations up until the final status of "destruction" .…”

Section: Combining Cellular Biomarkers Of Stressmentioning

confidence: 99%

Cadmium stress stimulates tissue turnover in Helix pomatia: increasing cell proliferation from metal tolerance to exhaustion in molluscan midgut gland

et al. 2010

View full text Add to dashboard Cite

In terrestrial pulmonate snails, cadmium (Cd) uptake leads to the induction of a Cd-specific metallothionein isoform (Cd-MT) that protects against adverse interactions of this toxic metal ion. Increasing concentrations of Cd cause increased individual mortality possibly linked to pathological alterations in the snail midgut gland. Histological, immuno-histochemical, and electron-microscopic methods in combination with tissue metal analyses and quantification of MT induction parameters were applied to the midgut gland of Cd-exposed Roman snails (Helix pomatia). Conspicuous concentration-dependent alterations occurred in this organ, including the metal-induced increase of Cd-MT concentration and manifestation of Cd-MT mRNA precipitations in all midgut gland cell types. The most evident alteration was an increase of cellular turnover reflected by enhanced cell proliferation. Intensified vesiculation of endoplasmic reticulum was noted in basophilic cells and an increasing formation of lipofuscin granules in excretory cells. At the highest Cd concentrations, mitochondrial membranes were disrupted in basophilic cells, and lipofuscin granules were released from excretory cells into the midgut gland tubular system. Some of these alterations (e.g., increased cell proliferation rate, vesiculation of endoplasmic reticulum) detected at low Cd concentrations were interpreted as adaptive response processes enhancing the tolerance of exposed individuals to metal stress. Cellular alterations at higher Cd concentrations (e.g., mitochondrial structural damage) clearly represented ongoing irreversible cellular disruption. Combined evaluation of cellular biomarkers and MT saturation levels indicated that the transition from stress resistance to depletion of resistance capacity occurred above a threshold of 0.8 micromol Cd/g dry weight in the midgut gland of H. pomatia. At these Cd concentrations, Cd-MT was saturated with Cd(2+) ions, whereas at the cellular level, structural alterations turned into pathological deterioration.

show abstract

Section: Combining Cellular Biomarkers Of Stressmentioning

confidence: 99%

Cadmium stress stimulates tissue turnover in Helix pomatia: increasing cell proliferation from metal tolerance to exhaustion in molluscan midgut gland

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Indeed, the digestive epithelium has been proposed to be fully regenerated every feeding cycle, which lasts a few hours (Nelson and Morton 1979). Regardless of whether such rapid cell renewal occurs, diverse sources of environmental stress have been demonstrated to cause a reorganisation of the epithelium resulting in an increased proportion of basophilic cells (Thompson et al 1974;Rasmussen et al 1983;Cajaraville et al 1990;Marigómez et al 1990Marigómez et al , 1998aSyasina et al 1997). This phenomenon, known as cell-type replacement (CTR), is clearly initiated after merely a few hours of environmental insult and constitutes a fully reversible process, with cell-type ratios returning to normal within a few days after the cessation of the stress (Marigómez et al 1998b).…”

Section: Introductionmentioning

confidence: 99%

Circatidal variation in epithelial cell proliferation in the mussel digestive gland and stomach

2004

View full text Add to dashboard Cite

Epithelial cell renewal in mussel ( Mytilus galloprovincialis, Lmk) digestive gland and stomach was investigated by bromodeoxyuridine (BrdU) immunohistochemistry. Mussels were exposed to 4 mg BrdU/l seawater continuously. Starting at 6 h after treatment, samples were collected every 2 h for 2 days and BrdU labelling was estimated by direct counting at the light microscope, with values being noted per thousand BrdU-positive cells. BrdU-positive reaction was observed in the nuclei of digestive, basophilic, duct and stomach cells, and in haemocytes. Cell renewal in digestive diverticula was synchronised following a circatidal pattern: BrdU labelling increased during low tide and decreased during high tide. Clearcut mitotic figures were identified in digestive cells, thereby confirming that mature cell types proliferate, in agreement with results from immunohistochemistry for proliferating cell nuclear antigen and BrdU. Epithelial cell renewal in the stomach also appeared to be synchronised.

show abstract

“…This is crucial in several organs and tissues with heterogeneous cell composition where only a certain cell type may be responsive to pollutants (e.g., the digestive gland of bivalve molluscs). It is also important in cases where pollutants affect cell type composition (e.g., replacement of digestive cells by basophilic cells in stressed molluscan digestive glands, Cajaraville et al, 1990b). Second, through the use of a set of cellular markers a global view of pollutant effects can be achieved as biochemical/molecular processes all become integrated at the cellular level.…”

Section: Peroxisome Proliferation As a Novel Cellular Marker Of Organmentioning

confidence: 99%

Peroxisome proliferation as a biomarker in environmental pollution assessment

Cajaraville

Cancio

Ibabe

et al. 2003

Microscopy Res & Technique

133

View full text Add to dashboard Cite

Peroxisome proliferators comprise a heterogeneous group of compounds known for their ability to cause massive proliferation of peroxisomes and liver carcinogenesis in rodents. In recent years it has become evident that other animals may be threatened by peroxisome proliferators, in particular aquatic organisms living in coastal and estuarine areas. These animals are exposed to a variety of pollutants of industrial, agricultural and urban origin which are potential peroxisome proliferators. Both laboratory and field studies have shown that phthalate ester plasticizers, PAHs and oil derivatives, PCBs, certain pesticides, bleached kraft pulp and paper mill effluents, alkylphenols and estrogens provoke peroxisome proliferation in different fish or bivalve mollusc species. The response appears to be mediated by peroxisome-proliferator activated receptors, members of the nuclear receptor family, recently cloned in fish. Based on these results it is proposed that peroxisome proliferation could be used as a biomarker of exposure to a variety of pollutants in environmental pollution assessment. This is illustrated by a case study in which mussels, used worldwide as sentinels of environmental pollution, were transplanted from reference to contaminated areas and vice versa. In mussels native to an area polluted with PAHs and PCBs, peroxisomal acyl-CoA oxidase (AOX) activity and peroxisomal volume density were 2-3 fold and 5-fold higher, respectively, compared to the reference site. When animals were transplanted to the polluted station, with increased concentration of organic xenobiotics, a concomitant significant increase of AOX was recorded. Conversely, in animals transplanted to the cleaner station, AOX activity and peroxisomal volume density decreased significantly. These results indicate that peroxisome proliferation is a rapid (i.e., two days) and reversible response to pollution in mussels. Before peroxisome proliferation can be implemented as a biomarker in biomonitoring programs, a well-defined protocol should be established and validated in intercalibration and quality assurance programmes. Furthermore, the influence of biotic and abiotic factors, some of which are known to affect peroxisome proliferation (season, tide level, interpopulation and interindividual variability), should be taken into consideration. The possible hepatocarcinogenic effects as well as the potential adverse effects on reproduction, development, and growth of peroxisome proliferators are unknown in aquatic organisms, thus providing a challenge for future investigations.

show abstract

Responses of basophilic cells of the digestive gland of mussels to petroleum hydrocarbon exposure

Cited by 79 publications

References 19 publications

Cadmium stress stimulates tissue turnover in Helix pomatia: increasing cell proliferation from metal tolerance to exhaustion in molluscan midgut gland

Cadmium stress stimulates tissue turnover in Helix pomatia: increasing cell proliferation from metal tolerance to exhaustion in molluscan midgut gland

Circatidal variation in epithelial cell proliferation in the mussel digestive gland and stomach

Peroxisome proliferation as a biomarker in environmental pollution assessment

Contact Info

Product

Resources

About