Responses of gill mitochondria-rich cells in Mozambique tilapia exposed to acidic environments (pH 4.0) in combination with different salinities

Furukawa, Fumiya; Watanabe, Shinji; Inokuchi, Mayu; Kaneko, Toyoji

doi:10.1016/j.cbpa.2010.12.003

Cited by 41 publications

(30 citation statements)

References 34 publications

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“…In type IV cells, NKCC1a could be an additional pathway for basolateral K ϩ absorption. The model was constructed in reference to previous observations (9,17,23,35). AC, accessory cell.…”

Section: Tilapia Romka and Romkb In Different Cladesmentioning

confidence: 99%

Gene expression and cellular localization of ROMKs in the gills and kidney of Mozambique tilapia acclimated to fresh water with high potassium concentration

Furukawa

Watanabe

Kakumura

et al. 2014

American Journal of Physiology-Regulatory, Integrative and Comp

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Regulation of plasma K(+) levels in narrow ranges is vital to vertebrate animals. Since seawater (SW) teleosts are loaded with excess K(+), they constantly excrete K(+) from the gills. However, the K(+) regulatory mechanisms in freshwater (FW)-acclimated teleosts are still unclear. We aimed to identify the possible K(+) regulatory mechanisms in the gills and kidney, the two major osmoregulatory organs, of FW-acclimated Mozambique tilapia (Oreochromis mossambicus). As a potential molecular candidate for renal K(+) handling, a putative renal outer medullary K(+) channel (ROMK) was cloned from the tilapia kidney and tentatively named "ROMKb"; another ROMK previously cloned from the tilapia gills was thus renamed "ROMKa". The fish were acclimated to control FW or to high-K(+) (H-K) FW for 1 wk, and we assessed physiological responses of tilapia to H-K treatment. As a result, urinary K(+) levels were slightly higher in H-K fish, implying a role of the kidney in K(+) excretion. However, the mRNA expression levels of both ROMKa and ROMKb were very low in the kidney, while that of K(+)/Cl(-) cotransporter 1 (KCC1) was robust. In the gills, ROMKa mRNA was markedly upregulated in H-K fish. Immunofluorescence staining showed that branchial ROMKa was expressed at the apical membrane of type I and type III ionocytes, and the ROMKa immunosignals were more intense in H-K fish than in control fish. The present study suggests that branchial ROMKa takes a central role for K(+) regulation in FW conditions and that K(+) excretion via the gills is activated irrespective of environmental salinity.

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“…In type IV cells, NKCC1a could be an additional pathway for basolateral K ϩ absorption. The model was constructed in reference to previous observations (9,17,23,35). AC, accessory cell.…”

Section: Tilapia Romka and Romkb In Different Cladesmentioning

confidence: 99%

Gene expression and cellular localization of ROMKs in the gills and kidney of Mozambique tilapia acclimated to fresh water with high potassium concentration

Furukawa

Watanabe

Kakumura

et al. 2014

American Journal of Physiology-Regulatory, Integrative and Comp

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“…However, some fish species prefer to live in acidic waters, such as the tambaqui, Colossoma macropomum (Cuvier) (Aride, Roubach, & Val, 2007), while others are acidic-tolerant, such as the Mozambique tilapia, Oreochromis mossambicus (Furukawa, Watanabe, Inokuchi, & Kaneko, 2011 Therefore, it is possible to have exceptions to that general rule of suitability of water pH. According to El-Sherif and El-Feky (2009), the optimal water pH for the culture of Nile tilapia, Oreochromis niloticus L., is 7 -8.…”

Section: Introductionmentioning

confidence: 99%

<b>Reassessment of the suitable range of water pH for culture of Nile tilapia <i>Oreochromis niloticus</i> L. in eutrophic water

Rebouças

Lima

Cavalcante

et al. 2016

Acta Sci. Anim. Sci.

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ABSTRACT. The present work aimed at reassessing the suitable range of water pH for culture of Nile tilapia, Oreochromis niloticus L. juveniles in eutrophic water. Two hundred and forty tilapia juveniles (1.37 ± 0.04 g) were stocked in twenty 250-L polyethylene tanks (12 fish per tank) for eight weeks. In the control tanks, the pH of water was not adjusted at any time, varying freely over the entire study. In the slight acidification treatment, the culture water was acidified daily to reach a water pH between 5.5 and 6.5. In the moderate acidification treatment, there were daily applications of HCl solution to reach a water pH between 4.5 and 5.5. In the alkalinization treatment, tanks received daily applications of Na 2 CO 3 and NaOH to achieve a water pH between 8.5 and 9.5. Acidification of water, regardless the degree, i.e., slight or moderate, was not able to significantly affect final body weight, specific growth rate and yield of fish. It can be concluded that the acidification of water up to pH 5.5 has no negative influence on growth of Nile tilapia fingerlings in eutrophic tanks. Accordingly, the suitable range of water pH for rearing Nile tilapia should be set at 5.5 -9.0.Keywords: pH of water, tilapia, aquaculture.Reavaliação da faixa adequada de pH da água para o cultivo da tilápia do Nilo, Oreochromis niloticus L. em águas eutróficas RESUMO. O presente trabalho teve por objetivo reavaliar a faixa adequada de pH da água para o cultivo de juvenis da tilápia do Nilo, Oreochromis niloticus L. em águas eutróficas. Foram estocados 140 juvenis de 1,37 ± 0,04 g em 20 tanques de polietileno de 250 L por oito semanas (12 peixes por tanque). Nos tanques do grupo controle, não houve ajuste do pH da água em nenhum momento, o qual variou livremente ao longo do estudo. Nos tanques submetidos à acidificação leve, a água de cultivo foi acidificada diariamente para atingir o valor de pH entre 5,5 e 6,5. Os tanques submetidos à acidificação moderada receberam aplicações diárias de solução de HCl para manter o pH da água entre 4,5 e 5,5. Nos tanques submetidos à alcalinização, procedeu-se a aplicação diária de Na 2 CO 3 e NaOH para manter o pH da água entre 8,5 e 9,5. A acidificação da água, independentemente do nível empregado, i.e., leve ou moderado, não foi capaz de afetar significativamente o peso corporal final, taxa de crescimento específico e produtividade de peixe. Concluiu-se que a acidificação da água até pH 5,5 não prejudica o crescimento de juvenis de tilápia em águas eutróficas. Por consequência, a faixa de adequação de pH da água para cultivo da tilápia do Nilo deveria ser estendida para 5,5 -9,0.Palavras-chave: pH da água, tilápia, aquicultura.

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“…In teleost and elasmobranch fishes, an ortholog or a species-specific paralog of NHE3 has been found to be localized in the apical membrane of ionocytes of acidtolerant Osorezan dace (Tribolodon hakonensis) (16), euryhaline Atlantic stingray (Dasyatis sabina) (9), zebrafish (49), Mozambique tilapia (Oreochromis mossambicus) (19), medaka (Oryzias latipes) (30), and rainbow trout (Oncorhynchus mykiss) (21). NHE3 expression in the gill or larvae increased when 1) Osorezan dace, Mozambique tilapia, and medaka were acclimated to acidic water (15)(16)30), 2) Atlantic stingray and medaka were transferred from seawater (SW) to FW (9), and 3) zebrafish and medaka were acclimated to low-Na ϩ FW (48 -49). Treating zebrafish larvae with an NHE inhibitor (5-ethylisopropyl amiloride, EIPA) reduced intracellular Na ϩ activity (aNa i ) of ionocytes (12), reduced 22 Na ϩ uptake by the larvae (25), and increased ionocyte-surface Na ϩ activity (i.e., reduced Na ϩ absorption) (42).…”

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confidence: 99%

Na⁺/H⁺and Na⁺/NH₄⁺exchange activities of zebrafish NHE3b expressed inXenopusoocytes

Ito

Kato

Hirata

et al. 2014

American Journal of Physiology-Regulatory, Integrative and Comp

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Zebrafish Na(+)/H(+) exchanger 3b (zNHE3b) is highly expressed in the apical membrane of ionocytes where Na(+) is absorbed from ion-poor fresh water against a concentration gradient. Much in vivo data indicated that zNHE3b is involved in Na(+) absorption but not leakage. However, zNHE3b-mediated Na(+) absorption has not been thermodynamically explained, and zNHE3b activity has not been measured. To address this issue, we overexpressed zNHE3b in Xenopus oocytes and characterized its activity by electrophysiology. Exposure of zNHE3b oocytes to Na(+)-free media resulted in significant decrease in intracellular pH (pH(i)) and intracellular Na(+) activity (aNa(i)). aNa(i) increased significantly when the cytoplasm was acidified by media containing CO₂-HCO₃(-) or butyrate. Activity of zNHE3b was inhibited by amiloride or 5-ethylisopropyl amiloride (EIPA). Although the activity was accompanied by a large hyperpolarization of ∼50 mV, voltage-clamp experiments showed that Na(+)/H(+) exchange activity of zNHE3b is electroneutral. Exposure of zNHE3b oocytes to medium containing NH₃/NH₄(+) resulted in significant decreases in pH(i) and aNa(i) and significant increase in intracellular NH₄(+) activity, indicating that zNHE3b mediates the Na(+)/NH₄(+) exchange. In low-Na(+) (0.5 mM) media, zNHE3b oocytes maintained aNa(i) of 1.3 mM, and Na(+)-influx was observed when pHi was decreased by media containing CO₂-HCO₃(-) or butyrate. These results provide thermodynamic evidence that zNHE3b mediates Na(+) absorption from ion-poor fresh water by its Na(+)/H(+) and Na(+)/NH₄(+) exchange activities.

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Responses of gill mitochondria-rich cells in Mozambique tilapia exposed to acidic environments (pH 4.0) in combination with different salinities

Cited by 41 publications

References 34 publications

Gene expression and cellular localization of ROMKs in the gills and kidney of Mozambique tilapia acclimated to fresh water with high potassium concentration

Gene expression and cellular localization of ROMKs in the gills and kidney of Mozambique tilapia acclimated to fresh water with high potassium concentration

<b>Reassessment of the suitable range of water pH for culture of Nile tilapia <i>Oreochromis niloticus</i> L. in eutrophic water

Na⁺/H⁺and Na⁺/NH₄⁺exchange activities of zebrafish NHE3b expressed inXenopusoocytes

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Responses of gill mitochondria-rich cells in Mozambique tilapia exposed to acidic environments (pH 4.0) in combination with different salinities

Cited by 41 publications

References 34 publications

Gene expression and cellular localization of ROMKs in the gills and kidney of Mozambique tilapia acclimated to fresh water with high potassium concentration

Gene expression and cellular localization of ROMKs in the gills and kidney of Mozambique tilapia acclimated to fresh water with high potassium concentration

<b>Reassessment of the suitable range of water pH for culture of Nile tilapia <i>Oreochromis niloticus</i> L. in eutrophic water

Na+/H+and Na+/NH4+exchange activities of zebrafish NHE3b expressed inXenopusoocytes

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Na⁺/H⁺and Na⁺/NH₄⁺exchange activities of zebrafish NHE3b expressed inXenopusoocytes