The cultivated soils in several semi-arid areas have very low organic matter due to climatic constraints that limit primary crop yield. Conservation tillage systems, outlined here as no tillage, no tillage with straw return and straw incorporation into the field, have been accepted as capable systems that preserve soil’s resources and sustain soil productivity. However, in semi-arid climates, there is presently no knowledge about the influence of different conservation tillage techniques on soil’s physical, chemical and biological properties at different soil depths in spring wheat fields and only little information about spring wheat yield in these management systems. Therefore, the present study was carried out with the objective of examining the impact of conservation tillage systems on soil properties (physical, chemical and biological) and spring wheat yield. The three conservation tillage treatments consisted of no tillage system (NT), wheat stubble return with no tillage (NTS) and straw incorporation with conventional tillage (CTS), as well as one conventional tillage (CT) control treatment, which were evaluated under randomized complete block design with three replications. The three conservation tillage treatments were compared with the conventional tillage control. Conservation tillage significantly increased the bulk density, gravimetric water content, water storage, hydraulic conductivity and soil aggregates and decreased the pore space and soil temperature compared to CT; however, no significant difference was found in the case of field capacity. Soil chemical properties in the 0–40 cm soil layer increased with conservation tillage compared to CT. Conservation tillage also notably increased the soil microbial counts, urease, alkaline phosphatase, invertase, cellulase and catalase activities relative to CT. Microbial biomasses (carbon and nitrogen) and wheat yield significantly elevated under conservation tillage compared to CT. Therefore, conservation tillage could significantly improve soil properties and maintain wheat yield for the research zone.