HANUŠ, O., VYLETĚLOVÁ, M., TOMÁŠKA, M., SAMKOVÁ, E., GENČUROVÁ, V., JEDELSKÁ, R., KOPECKÝ, J.: The eff ects of sample fat value manipulation on raw cow milk composition and indicators. Acta univ. agric. et silvic. Mendel. Brun., 2011, LIX, No. 1, pp. 101-112 Values of milk indicators (MIs) can be infl uenced by sampling errors and milk manipulation. This paper estimated the freezing point depression (FPD) and other MIs dri s which can cause fat movement. That is important for: -preparation of reference milk samples (MSs) for profi ciency testing and instrument calibrations; -estimation of the impact of milk treatment as centrifugation in dairy plants on FPD. Five MSs (A = original milk; milk with modifi ed fat (F) content; B = less F, C = low F, D = more F, E = high F) were created (gravitation F separation at 4 °C for 12 hours) with the same milk matrix 12× per year. F averages increased by 4.80% (122.1%) from 1.68 to 6.48% due to manipulation. It increased variability of MIs especially for SNF (solids non fat), L (lactose) and CP (crude protein). SCC (somatic cell count) averages increased by 803 (196.8%) from 9 to 812 thousand.ml −l . Correlation (r) F × SCC was 0.85 (P < 0.001). SNF, L and CP averages decreased by 0.47% (5.3%), 0.31% (6.3%) and 0.17% (5.0%). Correlations were −0.78, −0.75 and −0.64 (P < 0.001). Urea decreased along with F increase by 1.05 mg.100ml −l (2.9%) but with r −0.13 (P > 0.05). Acetone increased by 1.37 mg.l −l (47.6%) with r 0.21 (P > 0.05). Electrical conductivity decreased by 0.23 mS.cm −l (6.0%) with r −0.15 (P > 0.05). Alcohol stability was reduced by 0.14 ml (23.3%) with r −0.15 (P > 0.05). FPD, titration and actual acidity were not infl uenced.