Responses of Soil Microbial Population and Lignocellulolytic Enzyme Activities to Palm Kernel Shell Biochar Amendment

Halmi, Muhammad Farid Azlan; Simarani, Khanom

doi:10.1134/s1064229321120073

Cited by 6 publications

(2 citation statements)

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“…Biochar has been widely used as a soil amendment for soil fertility and crop productivity in many regions, including in the highly weathered soil of the humid tropics (Basak et al., 2022). Amendment of lignocellulosic biochar has been reported to ameliorate degraded tropical soil conditions (Apori & Byalebeka, 2021; Halmi & Simarani, 2024) and enhance agronomic production (Elias et al., 2020; Halmi et al., 2018). Therefore, biochar's application provides simultaneous benefits to soil amelioration in the humid tropics and waste recycling opportunities with climate mitigation potential.…”

Section: Introductionmentioning

confidence: 99%

“…The stability of biochar upon field soil application is a critical factor that decisively affects its carbon sequestration potential (Leng et al., 2019; Wang et al., 2016). Documenting the biodegradation of lignocellulosic biochar by soil microorganisms is essential to understanding its stability and persistence (Halmi & Simarani, 2021a; Wang et al., 2016). The cellulolytic degradation of lignocellulosic biomass involved a synergistic activity of microbial cellulase complex systems, which comprise endoglucanase (EC 3.2.1.4), exoglucanase (EC 3.2.1.91), and β‐glucosidase (EC 3.2.1.21) (Liu et al., 2021; Merlin et al., 2014).…”

Section: Introductionmentioning

confidence: 99%

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Response of soil microbial glycoside hydrolase family 6 cellulolytic population to lignocellulosic biochar reveals biochar stability toward microbial degradation

Halmi,

Simarani

2024

J of Env Quality

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Biochar produced from lignocellulosic biomass offers an opportunity to recycle waste into a valuable soil amendment. The application of biochar has been proposed to mitigate climate change by sequestering carbon in the soil. However, the field impact of biochar treatment on the cellulolytic microbial populations involved in the earlier steps of cellulose degradation is poorly understood. A field trial spanning three consecutive crop cycles of Zea mays was conducted in a degraded tropical Ultisol of Peninsular Malaysia. The soil was amended with two contrasting biochar made from oil palm kernel shells (pyrolyzed at 400°C) and rice husks (gasified at 800°C) with or without fertilizer supplementation. Soil samples were taken at each harvesting stage and analyzed for total organic carbon, labile active organic carbon, total cellulase, and β‐glucosidase. Microbial glycoside hydrolase family 6 (GH6) cellulase genes and transcripts, involved in the early steps of cellulose degradation, were quantified from the extracted soil deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), respectively. Total organic carbon, labile active organic carbon, and β‐glucosidase activity were significantly increased, while no effect on total cellulase activity was found. Both biochars stimulated the total population (DNA‐derived) abundance of soil microorganisms harboring the GH6 cellulase genes. The biochar amendment did not affect the active population (RNA‐derived) of the GH6 cellulolytic community, showing no significant changes in transcript expression. This indirectly corroborates the role of biochar as a potential carbon sequester in the soil.

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