Responsive fluorophore aggregation provides spectral contrast for fluorescence lifetime imaging

Schleyer, Kelton A.; Datko, Benjamin D.; Burnside, Brandon; Cui, Chao; Ma, Xiaowei; Grey, John K.; Cui, Lina

doi:10.1101/2020.01.28.923672

Cited by 1 publication

(1 citation statement)

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“…[566][567] Moreover, coupling ENS and disassembly would be an effective approach to mimic the cellular signal transduction cascades with feedback loops. 568 In addition, it would be fruitful to introduce other local changes (e.g., ligand-receptor interactions, 328,569 coordination, [570][571] pH-response, 494 ionic interaction, 572 redox reactions, 518,[573][574][575][576] bioorthogonal reactions 513,575,[577][578] , and dynamic covalent bonds [579][580][581][582][583] ) into the substrates of ENS for designing sophisticated molecular systems that can control emergent properties of molecular assemblies and modulate cellular functions.…”

Section: Structures Imaging and Modelingmentioning

confidence: 99%

Enzymatic Noncovalent Synthesis

et al. 2020

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Enzymatic reactions and noncovalent (i.e., supramolecular) interactions are two fundamental nongenetic attributes of life. Enzymatic noncovalent synthesis (ENS) refers to a process where enzymatic reactions control intermolecular noncovalent interactions for spatial organization of higher-order molecular assemblies that exhibit emergent properties and functions. Like enzymatic covalent synthesis (ECS), in which an enzyme catalyzes the formation of covalent bonds to generate individual molecules, ENS is a unifying theme for understanding the functions, morphologies, and locations of molecular ensembles in cellular environments. This review intends to provide a summary of the works of ENS within the last decade and emphasize ENS for functions. After comparing ECS and ENS, we describe a few representative examples where nature uses ENS, as a rule of life, to create the ensembles of biomacromolecules for emergent properties/functions in a myriad of cellular processes. Then, we focus on ENS of man-made (synthetic) molecules in cell-free conditions, classified by the types of enzymes. After that, we introduce the exploration of ENS of man-made molecules in the context of cells by discussing intercellular, peri/intracellular, and subcellular ENS for cell morphogenesis, molecular imaging, cancer therapy, and other applications. Finally, we provide a perspective on the promises of ENS for developing molecular assemblies/processes for functions. This review aims to be an updated introduction for researchers who are interested in exploring noncovalent synthesis for developing molecular science and technologies to address societal needs.

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