Responsive membranes for hydrophobic interaction chromatography

Himstedt, Heath H.; Qian, Xianghong; Weaver, Justin; Wickramasinghe, S. Ranil

doi:10.1016/j.memsci.2013.07.020

Cited by 40 publications

(44 citation statements)

References 45 publications

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“…PVCL-modified membranes achieve a BSA-binding capacity of 5 mg/mL, which is higher than that with nonmodified PVDF membranes and comparable to Sartorius hydrophobic membranes. The IgG-binding capacity is 21 mg/mL or approximately twice that of Sartorius membranes (75,77). To improve membrane performance, Wang and colleagues (78) developed a multimodal cationexchange membrane that employs both Coulombic and hydrophobic interactions.…”

Section: Hydrophobic and Multimodal Protein Purification Membranesmentioning

confidence: 99%

Functionalizing Microporous Membranes for Protein Purification and Protein Digestion

Dong

Bruening

2015

Annual Rev. Anal. Chem.

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This review examines advances in the functionalization of microporous membranes for protein purification and the development of protease-containing membranes for controlled protein digestion prior to mass spectrometry analysis. Recent studies confirm that membranes are superior to bead-based columns for rapid protein capture, presumably because convective mass transport in membrane pores rapidly brings proteins to binding sites. Modification of porous membranes with functional polymeric films or TiO₂ nanoparticles yields materials that selectively capture species ranging from phosphopeptides to His-tagged proteins, and protein-binding capacities often exceed those of commercial beads. Thin membranes also provide a convenient framework for creating enzyme-containing reactors that afford control over residence times. With millisecond residence times, reactors with immobilized proteases limit protein digestion to increase sequence coverage in mass spectrometry analysis and facilitate elucidation of protein structures. This review emphasizes the advantages of membrane-based techniques and concludes with some challenges for their practical application.

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Section: Hydrophobic and Multimodal Protein Purification Membranesmentioning

confidence: 99%

Functionalizing Microporous Membranes for Protein Purification and Protein Digestion

Dong

Bruening

2015

Annual Rev. Anal. Chem.

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“…However, membrane capacity is typically lower compared to that of resin. Significant efforts have been dedicated to develop high binding capacity and/or high recovery membrane adsorbers by grafting ligands on membrane substrates using UV-initiated polymerization or atom-transfer radical polymerization (ATRP) [8][9][10][11][12][13][14][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies [24][25][26][27] conjugated a monomeric acid, base or affinity ligand directly to the functional group on the substrate. However, there has been significant work [8][9][10][11][12][13][14][17][18][19] on surface modification by grafting polymers on the flat sheet membrane substrates using ATRP and UV-initiated polymerizations. These results show that there exists an optimal ligand chain density and chain length for maximizing protein binding capacities for IEX, affinity and HIC membrane chromatography [19].…”

Section: Introductionmentioning

confidence: 99%

“…However, there has been significant work [8][9][10][11][12][13][14][17][18][19] on surface modification by grafting polymers on the flat sheet membrane substrates using ATRP and UV-initiated polymerizations. These results show that there exists an optimal ligand chain density and chain length for maximizing protein binding capacities for IEX, affinity and HIC membrane chromatography [19]. Surface modification with UV initiated reaction or polymerization is fast and robust with extensive application for IEX membranes [28,29].…”

Section: Introductionmentioning

confidence: 99%

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Electrospun Weak Anion-Exchange Fibrous Membranes for Protein Purification

2020

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Membrane based ion-exchange (IEX) and hydrophobic interaction chromatography (HIC) for protein purification is often used to remove impurities and aggregates operated under the flow-through mode. IEX and HIC are also limited by capacity and recovery when operated under bind-and-elute mode for the fractionation of proteins. Electrospun nanofibrous membrane is characterized by its high surface area to volume ratio and high permeability. Here tertiary amine ligands are grafted onto the electrospun polysulfone (PSf) and polyacrylonitrile (PAN) membrane substrates using UV-initiated polymerization. Static and dynamic binding capacities for model protein bovine serum albumin (BSA) were determined under appropriate bind and elute buffer conditions. Static and dynamic binding capacities in the order of~100 mg/mL were obtained for the functionalized electrospun PAN membranes whereas these values reached~200 mg/mL for the functionalized electrospun PSf membranes. Protein recovery of over 96% was obtained for PAN-based membranes. However, it is only 56% for PSf-based membranes. Our work indicates that surface modification of electrospun membranes by grafting polymeric ligands can enhance protein adsorption due to increased surface area-to-volume ratio.

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“…Lysozyme dynamic binding capacity and recovery were determined following the same procedure as before. 29 The amount of protein bound was calculated by subtracting the washed out protein from the amount loaded. Recovery was calculated by dividing the amount of protein eluted by the total amount of protein bound.…”

Section: Introductionmentioning

confidence: 99%

Membrane Surface Engineering for Protein Separations: Experiments and Simulations

Liu

Wickramasinghe

et al. 2014

Langmuir

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A bisphosphonate derived ligand was successfully synthesized and grafted from the surface of regenerated cellulose membrane using atom transfer radical polymerization (ATRP) for protein separations. This ligand has a remarkable affinity for arginine (Arg) residues on protein surface. Hydrophilic residues N-(2-hydroxypropyl) methacrylamide (HPMA) was copolymerized to enhance the flexibility of the copolymer ligand and further improve specific protein adsorption. The polymerization of bisphosphonate derivatives was successful for the first time using ATRP. Static and dynamic binding capacities were determined for binding and elution of Arg rich lysozyme. The interaction mechanism between the copolymer ligand and lysozyme was elucidated using classical molecular dynamics (MD) simulations.

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Responsive membranes for hydrophobic interaction chromatography

Cited by 40 publications

References 45 publications

Functionalizing Microporous Membranes for Protein Purification and Protein Digestion

Functionalizing Microporous Membranes for Protein Purification and Protein Digestion

Electrospun Weak Anion-Exchange Fibrous Membranes for Protein Purification

Membrane Surface Engineering for Protein Separations: Experiments and Simulations

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