Responsiveness of the Pituitary Gland to Androgens and of the Testis to Gonadotrophins Following Damage to Spermatogenesis in Rats

Main, S. J.; Setchell, B. P.

doi:10.1677/joe.0.0870445

Cited by 23 publications

(18 citation statements)

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“…The authors claimed that the modifications of Leydig cell function after efferent duct ligation are mainly due to local changes within the testes, providing further evidence for an intratesticular control of Leydig cell function. However, since there was a concurrent tendency of increment in testosterone concentration both in testicular venous blood and testicular tissue fluid of the contralateral control testis at 21 days after unilateral efferent duct ligation and Main & Setchell (1980) found a suggestive rise in serum LH in such circumstances, it seems that the gonadotrophic system rather than a local mechanism is involved.…”

Section: Discussionmentioning

confidence: 94%

Effect of surgery and efferent duct ligation on testicular blood flow and testicular steroidogenesis in the rat

Wang¹,

Gu²,

Lin³

et al. 1985

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A moderate reduction in testicular blood flow was observed in both testes 24 h after unilateral efferent duct ligation without any corresponding change in testosterone secretion as indicated in the peripheral blood, in testicular venous blood, or in testicular tissue fluid. At 21 days a pronounced unilateral decrease in blood flow was associated with the extensive degeneration of tubules in the testis on the ligated side. These changes were also associated with decreased testosterone secretion by the testis on the ligated side, although Leydig cell function was not abolished since testosterone in the tissue increased rather than decreased. It is therefore concluded that testicular blood flow may play an important role in the changes of testosterone secretion that follow unilateral efferent duct ligation.

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Section: Discussionmentioning

confidence: 94%

Effect of surgery and efferent duct ligation on testicular blood flow and testicular steroidogenesis in the rat

Wang¹,

Gu²,

Lin³

et al. 1985

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“…Sampling was started 15 sec before the microspheres labelled with 57Co (New England Nuclear, Dreiech, West Germany; 150 000 microspheres containing 5 µ in 0-1 ml 0-154 M-NaCl, diluted and thoroughly mixed by vortexing) were injected into the aortic arch. A reference blood sample from the femoral artery was collected for 3 min after the injection, and then blood samples were taken from both testicular veins, as described by Main & Setchell (1980). Briefly, the superficial vein on the testicular wall was punctured gently with a sharp injection needle, so that venous blood could be collected into a plastic vial by means of a glass capillary.…”

Section: Introductionmentioning

confidence: 99%

Effect of gossypol on testicular blood flow and testosterone production in rats

Wang¹,

Qian²,

Gao³

1984

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Rats were treated with highly purified gossypol acetic acid at doses of 15 or 30 mg/kg day-1 for 6 weeks to produce an effect on spermatogenesis as shown by reduced sperm motility and increased sperm malformation rates. The treated rats did not differ from the controls in the body weight growth curves and reproductive organ weights. When stimulated with hCG, testicular blood flow was increased in the low dose group; the testosterone concentrations in peripheral and testicular venous blood were also increased to a greater extent than those of the control group. No difference was found between the high dose and control groups in testicular blood flow or testosterone concentrations. The morphology of the Leydig cells was apparently normal, although some degenerative changes in the germinal epithelium were observed in the high dose group. Therefore, there is no evidence in our experiment to show any anti-androgenic effect following 6-week treatment of gossypol in rats, even at the dose of 30 mg/kg day-1.

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“…The procedures used have been described in detail elsewhere (Main and Setchell 1980;Wang et af. 1983).…”

Section: Methodsmentioning

confidence: 99%

“…The intra-assay and interassay coefficients of variation were 6·2, 6·1 and 6·1 % and 15·7, 21·7 and 20·1 % respectively and the sensitivities were 24·8, 1·6 ng/ml and 0·46 pmol/ml respectively. Details of the methods used are given elsewhere (Main and Setchell 1980). Plasma flow was calculated from blood flow by multiplying this flow by (1-haematocrit).…”

Section: Methodsmentioning

confidence: 99%

“…Following a variety of treatments which 'selectively' damage the tubules (cryptorchidism- Risbridger et al 1981a; fetal irradiation, vitamin A deficiency, administration of hydroxyurea- Rich et al 1979; efferent duct ligation- Main and Setchell 1980;Risbridger et al 1981b; local heating-Main and Setchelll980; Galil and Setchelll980, 1981;or local irradiation -Wang et al 1983) the Leydig cells appear hypertrophic and, furthermore, if the aspermatogenic testes are decapsulated or Leydig cells are isolated from them and incubated in vitro, they produce normal or often greater than normal amounts of testosterone. On the other hand, the concentration of testosterone in the peripheral blood is normal or slightly subnormal despite substantial increases in the concentration of luteinizing hormone (LH) in the blood, and the aspermatogenic testes appear to be less able to respond to stimulation with injections of human chorionic gonadotrophin (heG), at least as judged by the concentrations of testosterone in the peripheral circulation.…”

Section: Introductionmentioning

confidence: 99%

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Limitations Imposed by Testicular Blood Flow on the Function of Leydig Cells in Rats in vivo

Bp¹,

Ka²

1983

Aust. Jnl. Of Bio. Sci.

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Testis blood flow per testis closely follows testis weight in rats made aspermatogenic by a single exposure of the testis to 43°C for 30 min or 500 rad (5 Gy) of irradiation from a caesium source, or following ligation of the efferent ducts. Aspermatogenesis following these treatments was associated with only minor changes in the concentrations of testosterone in peripheral blood before stimulation with human chorionic gonadotrophin (hCG), and a reduced responsiveness to hCG when testis weight had fallen after heating. The concentrations of testosterone in testicular venous blood was normal or above normal during aspermatogenesis resulting from heat or irradiation, and only slightly reduced following efferent duct ligation.Consequently testosterone production (defined as the product of plasma flow and the venoarterial concentration difference for testosterone) was markedly reduced during aspermatogenesis, both before and after stimulation with hCG. It appears that the reduced blood flow limits the amount of testosterone leaving the testis, and while the Leydig cells are capable under some circumstances of compensating partially for this fall by increasing the concentration of testosterone in the testicular venous blood, this compensation is not complete when there are severe reductions in blood flow. Therefore one can conclude that the mass of the tubules is the main determinant of testis blood flow and the Leydig cells must manage with what the tubules require.

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Responsiveness of the Pituitary Gland to Androgens and of the Testis to Gonadotrophins Following Damage to Spermatogenesis in Rats

Cited by 23 publications

References 0 publications

Effect of surgery and efferent duct ligation on testicular blood flow and testicular steroidogenesis in the rat

Effect of surgery and efferent duct ligation on testicular blood flow and testicular steroidogenesis in the rat

Effect of gossypol on testicular blood flow and testosterone production in rats

Limitations Imposed by Testicular Blood Flow on the Function of Leydig Cells in Rats in vivo

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