Respuesta fisiológica de Euglena gracilis al estrés por cobre

García, David Cervantes; González-Mendoza, Daniel; Cervantes-Díaz, Lourdes; Morales, Adriana; Grimaldo-Juarez, Onecimo; Zamora-Bustillos, Roberto

doi:10.1590/s0100-40422011000700020

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…The molecular mechanism of the reduction in pigment levels may involve the accumulation of lipophilic anthracene in thylakoid membranes 39 , resulting in conformational changes in their structure and composition. In general, reduced pigmentation under chemical stress results from inhibition of enzymes related to chlorophyll synthesis, degradation of chlorophyll and DNA damage 40 , or accelerated degradation of pigments due to increased ROS formation at various positions in the photosynthetic electron transport chain. Moreover, carotenoids prevent photo-oxidative destruction of chlorophylls 41 and, therefore, a reduction in carotenoids could have additional serious consequences on chlorophyll molecules.…”

Section: Resultsmentioning

confidence: 99%

Anthracene phytotoxicity in the freshwater flagellate alga Euglena agilis Carter

Kottuparambil

Park

2019

Sci Rep

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The freshwater flagellate alga Euglena agilis Carter was exposed to the polycyclic aromatic hydrocarbon (PAH) anthracene for 96 h under optimal photosynthetically active radiation (PAR), and responses of growth, photosynthetic pigment production, and photosynthetic efficiency were assessed. Anthracene reduced the growth rate (μ) and levels of chlorophyll a (Chl a), chlorophyll b (Chl b), and total carotenoids. The growth rate was more sensitive than photosynthetic parameters, with a median effective concentration (EC50) of 4.28 mg L−1. Between 5 and 15 mg L−1, anthracene inhibited the maximum quantum yield (Fv/Fm) of photosystem II (PSII) and the maximum photosynthetic electron transport rate through PSII (rETRmax) with EC50 values of 14.88 and 11.8 mg L−1, respectively. At all anthracene concentrations, intracellular reactive oxygen species (ROS) were elevated, indicating increased oxidative stress. Anthracene presumably reduced the PSII efficiency of photochemical energy regulation and altered the photochemistry through intracellular ROS formation. Acute exposure to PAHs may induce severe physiological changes in phytoplankton cells, which may influence vital ecological processes within the aquatic environments. Additionally, growth and Chl a content may serve as sensitive risk assessment parameters of anthracene toxicity in water management since EC50 values for both overlap with anthracene levels (8.3 mg L−1) permitted by the US Environmental Protection Agency (USEPA).

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Section: Resultsmentioning

confidence: 99%

Anthracene phytotoxicity in the freshwater flagellate alga Euglena agilis Carter

Kottuparambil

Park

2019

Sci Rep

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Evaluacion de la bioacumulacion de cobre en Euglena gracilis mediante la tecnica de fluorescencia de rayos X

Cervantes-Garcia

Rubalcaba-Sil

González-Mendoza

et al. 2014

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RESUMEN.En esta investigación se evaluó el efecto de la exposición de cobre en la capacidad de bioacumulacion de metal en células de Euglena gracilis. La acumulación de metal medida con la técnica de fluorescencia de rayos X (XRF) mostró que la acumulación de Cu +2 en E. gracilis fue dosis dependiente y se incrementó significativamente en las células tratadas con 0.4 y 0.8 mM de Cu +2 con respecto al control. No se descarta la presencia de una estrategia de acumulación en E. gracilis que podría involucrar la participación de una serie de múltiples procesos, como producción de vacuolas. Futuros estudios al respecto deberán orientarse a evaluar la capacidad de bioacumulación de E. gracilis para su aplicación en programas de biorremediación de sistemas acuáticos. Palabras clave: Euglena gracilis, XRF, cobre, contaminación, tolerancia, ecotoxicología. Assessment of copper bioaccumulation in Euglena gracilisby X-Ray fluorescence technique ABSTRACT. The effect of the exposure to copper in metal bioaccumulation capacity in cells from Euglena gracilis was evaluated in this study. Metal accumulation measured with the X-ray fluorescence (XRF) technique showed that the accumulation of Cu +2 in E. gracilis was dose dependent and was significantly increased in cells treated with 0.4 and 0.8 mM of Cu +2 with respect to control. The presence of an accumulation strategy in E. gracilis that could involve the participation of a number of multiple processes, such as production of vacuoles, is not discarded. Future studies should be oriented to evaluate the potential for bioaccumulation of E. gracilis to be applied in bioremediation of aquatic systems programs.

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Copper induced biofilm formation and changes on photosynthetic pigment in Euglena gracilis

Morales-Calderon¹,

Armenta-Ortiz²,

Méndez-Trujillo³

et al. 2012

Afr. J. Microbiol. Res.

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In the present study we evaluated the effects of two copper sulfate (CuSO 4) concentrations (0.4 and 3 mM) in the culture medium on the biofilm production and photosynthetic compounds concentrations in Euglena gracilis. The results showed that the exposure of E. gracilis to both 0.4 and 3 mM Cu 2+ stimulated the ability of this microorganism to increase its biofilm production with a mean ± SD optical density measured at 590 nm (0.38 ± 0.04, and 0.27 ± 0.07, respectively). In contrast when E. gracilis was exposed to 0.4 and 3 mM Cu 2+ respectively, the growth of cells and chlorophyll a and b content showed significant decrease relative to those showed by the control. These results suggest that the biofilm formation could be considered as metal protection mechanisms in E. gracilis. Finally, these results provide useful and important information for researchers in order to increase the eco-toxicological studies about E. gracilis for its employment in bioremediation programs.

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Respuesta fisiológica de Euglena gracilis al estrés por cobre

Cited by 3 publications

References 10 publications

Anthracene phytotoxicity in the freshwater flagellate alga Euglena agilis Carter

Anthracene phytotoxicity in the freshwater flagellate alga Euglena agilis Carter

Evaluacion de la bioacumulacion de cobre en Euglena gracilis mediante la tecnica de fluorescencia de rayos X

Copper induced biofilm formation and changes on photosynthetic pigment in Euglena gracilis

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