Restoration of nitric oxide production by aldose reductase inhibitor in human endothelial cells cultured in high-glucose medium

Ferulic acid, a natural polyphenol, alleviates insulin resistance and hypertension in fructose fed rats: Effect on endothelial-dependent relaxation

“…This is in accordance with the reported restoration of NO production by AR inhibition in human umbilical endothelial cells cultured in a medium with high glucose content [40]. These assumptions are also supported by previous work in which ZOP inhibited the reduction in flow-induced dilation induced by high glucose [36] and restored NO production in ischemic myocardial injury [41].…”

Advanced glycation end products quench nitric oxide in vitro

“…Intracellular glycation may alter eNOS activity either transcriptionally or posttranslationally. This concept is currently under study [27,32]. The results of Rojas et al indicated that eNOS expression is reduced by AGEs by increasing the rate of mRNA degradation and may be relevant to the impairment of some endothelial functions observed in diabetes and ageing.…”

Molecular mechanisms of glutamine action

“…Glucosamine, the main bioproduct of the hexosamine biosynthetic pathway, inhibits nitric oxide synthesis in endothelial cells. Moreover, increasing extracellular concentrations of both glutamine and glucose resulted in decreased nitric oxide production by endothelial cells (Giugliano et al, 1997;Okuda et al, 1997;Cosentino and Luscher, 1998;Mitchell et al, 2000), suggesting that the glucosamine biosynthetic pathway mediates the inhibition of endothelial nitric oxide synthesis induced by hyperglycemia and high plasma glutamine levels (Wu et al, 2001).…”