Restoration of ovarian function after autotransplantation of intact frozen-thawed sheep ovaries with microvascular anastomosis

Whole sheep ovary cryopreservation: evaluation of a slow freezing protocol with dimethylsulphoxide

“…A standard procedure for evaluation of cryopreserved whole ovaries after thawing is light microscopy, which has been used in the sheep [14][15][16]24] and human [7,23]. In the present study a higher density of small follicles was seen after cryopreservation in DMSO as compared to RA, further pointing towards the necessity of inclusion of a CPA.…”

“…The primordial follicular viability after cryopreservation of whole ovine ovary using DMSO as assessed with Trypan blue test was 83% [14] and with the same test the viability was 76% after thawing of the whole human frozen ovary [7]. In studies of cryopreserved ovarian cortical biopsies from PCOS patients a similar proportion of live follicles was seen [48].…”

“…In our present study we used the common method of placing the frozen vial into a water bath of 37°C [14,49] Further studies are needed to define thawing procedures corresponding to adequate freezing protocol.…”

“…Research on whole ovary transplantation has been performed in a number of animal models [8][9][10][11][12], including the rhesus macaque [13]. The sheep is the species that has been used most extensively in research on whole ovary cryopreservation [14][15][16].…”

“…Two chemically different cryoprotectants, dimethylsulphoxide (DMSO) [7,14,16,23] and propanediol [24] have mainly been used in whole ovary cryopreservation research. However, there is a lack of systematic studies that have investigated the different facets of whole organ cryopreservation.…”