Restoration of specific immunity against SV40 tumor‐specific transplantation antigen to lymphoid cells from tumor‐bearing mice

Abstract

Specific cell-mediated immunity to SV40 tumor-specific transplantation antigen (TSTA) in BALB/c mice undergoing progressive tumorigenesis by syngeneic SV40-transformed cells (VLM) was investigated in vivo using a tumor-cell neutralization test. Specific cellular reactivity to SV40 TSTA was not detected in BALB/c mice bearing large tumors (10-15 mm mean diameter) but was demonstrable after tumor excision. Specific cytotoxic reactivity against syngeneic SV40-transformed cells in vivo could be restored to lymphoid cells from VLM tumor-bearing mice either by culturing the lymphoid cells in vitro or by treating them with papain or trypsin. Enzyme-treated lymphoid cells from MCA tumor-bearing BALB/c mice had no cytotoxic reactivity against VLM cells. These studies suggest that tumor-bearing hosts possess lymphocytes which are sensitized to the TSTA of the tumor but that the reactivity of these lymphocytes is blocked.

Immunopathology

“…Preincubation of lymphoid cells in serum-free medium, washing, or treatment with enzymes, restored cellular activity in some of these reports (Prather and Lausch, 1977;Blasecki and Tevethia, 1975a,b). In addition, excision of tumor restored activity in the SV40-mouse system (Blasecki and Tevethia, 1975a;Howell et al, 1975). In summary, it appears that specific cell-mediated immunity is present during the early stages of tumor development.…”

“…These include colony inhibition (CI) in the polyoma (Hellstrom and Sjogren, 1965), Shope papilloma (Hellstrom et ai., 1969), RSV (Jonsson, 1969), MSV (Hellstrom and Hellstrom, 1969), SV40 (Tevethia et ai., 1970), and adenovirus (Berman, unpublished observations) systems; microcytotoxicity assay (MCA) in the MTV (Lane et ai., 1975), RSV (Bellone and Pollard, 1970), polyoma (Mullen et ai., 1975;Walla and Lamon, 1977), SV40 (Smith et ai., 1970), and MSV systems; CRA in the MSV (Chuat et al, 1969;Burstein, 1970;Leclerc et ai., 1972), Shope fibroma (Singh et ai., 1972) adenovirus (Ankerst, 1971), and SV40 (Wright and Law, 1971) systems; macrophage migration inhibition (MMI) in the SV40 (Pekarek et al, 1968), adenovirus (Rees and Potter, 1973), Shope fibroma (Tompkins et ai., 1970a), and MSV (Landolfo et ai., 1977) systems; the isotopic footpad assay in the SV 40 (Paranjpe and Boone 1972) system, the leukocyte adherence inhibition (LAI) assay in the RSV (Wikstrand et ai., 1978) and MSV (Koppi and Halliday, 1981) systems and adoptive transfer (or the Winn Test) in the adenovirus (Berman 1967), Polyoma (Vandeputte and Datta 1972), RSV (Jonsson, 1971), and SV40 (Blasecki and Tevethia, 1975a) systems.…”

“…In contrast, it was shown that animals with large tumors tended to lose cellular immunity by CI in the RSV-rat system (Jonsson, 1973), by the CRA in the MSV-mouse and RSV-chicken systems (Leclerc et ai., 1972;McGrail et al, 1978), by the Winn (Howell et al, 1975;Blasecki and Tevethia, 1975a) and MMI tests (Blasecki and Tevethia, 1975b) and direct tumor challenge (Howell et al, 1975) in the SV40-mouse system, and by MCA in the adenovirus-SV40 hybrid virus-hamster system (Prather and Lausch, 1977). Preincubation of lymphoid cells in serum-free medium, washing, or treatment with enzymes, restored cellular activity in some of these reports (Prather and Lausch, 1977;Blasecki and Tevethia, 1975a,b).…”

Cell‐mediated Immunity to Mouse Tumors: Some Recent Findings*

“…If further confirmed, they may help explain why lymphoid cells from mice with large tumors often lack detectable reactivity in vitro,l6, lo why incubation of such lymphoid cells in vitro can sometimes restore this reactivity,5 and why treatment of lymphocytes from tumor-bearing animals with papain sometimes has a similar effect. 16 Further work is obviously needed to investigate the nature of these blocking factors as well as the nature of the cells carrying them, both with respect to whether the blocking factors are primarily present on T cells, B cells, K cells, or on cells representing some other type. It needs also to be investigated how…”

Biological characterization of a tumour‐growth‐promoting factor from ascitic fluid of tumour‐bearing mice

“…The time from removal of the tumour to reappearance of immunity is approximately 10 days (Howell et al, 1975). Similarly, Blasecki and Tevethia (1975) observed that specific immunological reactivity could be restored to lymphoid cells from tumour-bearing animals in eclipse phase by culturing them in vitro or by treating them with papain or trypsin.…”