Restoration of Strength Despite Low Stress and Abnormal Imaging after Achilles Injury

Trudel, Guy; Doherty, Geoffrey P.; Koike, Yoichi; Ramachandran, Nanthan; Lecompte, Martin; Dinh, Laurent; Uhthoff, Hans K.

doi:10.1249/mss.0b013e3181a706f0

Cited by 16 publications

(17 citation statements)

References 36 publications

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“…Therefore, the improved linear fiber pattern scores in fdESC treated tendons demonstrate significant and clinically relevant superior healing in the fdESC group, and suggest at least faster injury resolution, if not an improvement in long term outcome. Although there is little available data on the MRI appearance of healing tendons, it is known experimentally that reduced lesion signal intensity is correlated with tendon mechanical recovery [41] and reduction in pain [42]. Therefore, the trend toward reduced MR signal intensity (one-tailed P = 0.07) and trend for reduced lesion and relative CSA on MRI (one-tailed P = 0.06) in fdESC treated tendons corroborates better tendon injury resolution.…”

Section: Discussionmentioning

confidence: 72%

Fetal derived embryonic-like stem cells improve healing in a large animal flexor tendonitis model

et al. 2011

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IntroductionTendon injury is a common problem in athletes, with poor tissue regeneration and a high rate of re-injury. Stem cell therapy is an attractive treatment modality as it may induce tissue regeneration rather than tissue repair. Currently, there are no reports on the use of pluripotent cells in a large animal tendon model in vivo. We report the use of intra-lesional injection of male, fetal derived embryonic-like stem cells (fdESC) that express Oct-4, Nanog, SSEA4, Tra 1-60, Tra 1-81 and telomerase.MethodsTendon injury was induced using a collagenase gel-physical defect model in the mid-metacarpal region of the superficial digital flexor tendon (SDFT) of eight female adult Thoroughbred or Thoroughbred cross horses. Tendon lesions were treated one week later with intra-lesional injection of male derived fdESCs in media or media alone. Therapy was blinded and randomized. Serial ultrasound examinations were performed and final analysis at eight weeks included magnetic resonance imaging (MRI), biochemical assays (total DNA, glycosaminoglycan, collagen), gene expression (TNC, TNMD, SCX, COL1A1, COL3A1, COMP, DCN, MMP1, MMP3, MMP13, 18S) and histology. Differences between groups were assessed with Wilcoxon's rank sum test.ResultsCell survival was demonstrated via the presence of the SRY gene in fdESC treated, but not control treated, female SDFT at the end of the trial. There were no differences in tendon matrix specific gene expression or total proteoglycan, collagen or DNA of tendon lesions between groups. Tissue architecture, tendon size, tendon lesion size, and tendon linear fiber pattern were significantly improved on histologic sections and ultrasound in the fdESC treated tendons.ConclusionsSuch profound structural effects lend further support to the notion that pluripotent stem cells can effect musculoskeletal regeneration, rather than repair, even without in vitro lineage specific differentiation. Further investigation into the safety of pluripotent cellular therapy as well as the mechanisms by which repair was improved seem warranted.

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Section: Discussionmentioning

confidence: 72%

Fetal derived embryonic-like stem cells improve healing in a large animal flexor tendonitis model

et al. 2011

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“…The p value s of significant results are reported in the Results and the complete data are shown in the tables (histopathology and ultrastructure) and graphs (diameter of the injured area, dry matter content and biomechanical findings) to minimize duplication of the data. For those base scoring data, the median and minimum-maximum of the scores are reported in the Results [median (min-max)] [Trudel et al, 2009].…”

Section: Discussionmentioning

confidence: 99%

“…The cross-sectional echo-texture of the SDFT of the rabbits was not diagnostic because of their low diameter and view; therefore, the animals were sonographed at longitudinal section with a 12-MHz linear probe (SLR-400; Siemens, Berlin, Germany) and Echowave 3.23 software according to the methodology published by Trudel et al [2009]. Briefly, the following criteria and directions were used to define differences between the injured tendons and their normal contralateral counterparts: (i) US echogenicity of the tendon; (ii) ratio of the hyperechogenic area of the echotexture of the tendons to the hypoechogenic area; (iii) mobility of the tendon as an index of peritendinous adhesion ( table 2 ), and (iv) the diameter of the SDFT was calculated using the scale of the US device [Trudel et al, 2009;Moshiri and Oryan, 2011;. obvious tenderness, bowed and warm; color changed severe n = 20 for each group.…”

Section: Ultrasonographymentioning

confidence: 99%

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Novel Application of Theranekron® Enhanced the Structural and Functional Performance of the Tenotomized Tendon in Rabbits

Oryan¹,

Moshiri

Raayat

2012

Cells Tissues Organs

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The effects of Tarantula cubensis extract (TC; Theranekron®) on the experimentally induced rupture of the superficial digital flexor tendon (SDFT) 28 days post-injury (DPI) was studied in rabbits. Forty mature White New Zealand male rabbits were randomly divided into two groups. TC was repeatedly injected subcutaneously over the lesion 3, 7 and 10 days after tenotomy and surgical anastomosis. Clinical and ultrasonographic evaluations were conducted at weekly intervals. The animals were euthanized 28 DPI and the tendons were investigated for macroscopic, histopathologic, ultrastructural, biomechanical and percent dry weight parameters. Treatment reduced signs of acute inflammation and strongly ameliorated clinical symptoms, structural organization and biomechanical properties (p < 0.05). Apparently, TC is effective in restoring the clinical, morphological and biomechanical properties of the injured SDFT in rabbits and may be valuable in human and veterinary medicine.

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“…37,38,39 The skin samples were harvested immediately after euthanasia and placed between sterile sponges soaked with 0.9% saline, to preserve the normal tissue hydration after harvesting. They were then placed in occlusive bags and stored at -70°C prior to testing.…”

Section: Biomechanical Testingmentioning

confidence: 99%

Cutaneous Wound Healing after Topical Application of <i>Pistacia atlantica</i> Gel Formulation in Rats

Hamidi

Naeini

Oryan

et al. 2017

tjps

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ÖZAmaç: Bu çalışma, sıçanlarda oluşturulan deneysel yara modelinde Pistacia atlantica yağı ile hazırlanmış jel formülasyonunun, enzimatik antioksidanlar üzerindeki etkisini araştırmak amacıyla yapılmıştır. Gereç ve Yöntemler: Genel anestezi altına alınan hayvanlarda, aseptik koşullarda, insizyon yapmak suretiyle kare şeklinde yaralar (2x2 cm) oluşturulmuştur. Daha sonra, hayvanlar rasgele dört gruba ayrılmıştır (I-tedavi edilmemiş kontrol grubu, II-haricen uygulanan baz jel grubu, IIIharicen %5 jel uygulanan grup, IV-haricen %10 jel uygulanan grup). Bulgular: Sonuçlar, Pistacia atlantica yağı ile hazırlanmış jel formülasyonunun haricen uygulanmasının, kontrol ve baz jel gruplarına kıyasla, bütünlüğü bozulmamış stratum bazalisi, olgun granülasyon dokusunu, kıl folikülleri ve ter bezleri ile reepitelizasyonu arttırdığını göstermektedir. Yara oluşumundan 21 gün sonra, %10 yağlı jel ile tedavi edilen hayvanların dokularındaki gerilme kuvveti, kopma gerilimi ve bükülme direnci, kontrol ve baz jel grubu ile karşılaştırıldığında önemli ölçüde artmıştır. Aynı evredeki kontrol ve baz jel ile muamele edilen gruplara kıyasla, kollajen liflerin daha organize bir düzenlenme ve dokunun daha düzgün bir iyileşme gösterdiği belirlenmiştir. Sonuç: Pistacia atlantica yağ jelinin haricen uygulanmasının, sıçanlarda oluşturulan deneysel yaralar üzerinde morfolojik, biyokimyasal ve biyomekanik yönden düzeltici rol oynadığı ortaya koyulmuştur. Anahtar kelimeler: Pistacia atlantica, yara iyileşmesi, histopatoloji, biomekanik, deri Objectives: This study has been undertaken to investigate the antioxidant activity of the gel formulation from Pistacia atlantica oil extraction on enzymatic antioxidants in experimental wound created in rats. Materials and Methods: Under anesthesia, a square-shaped skin defect (2x2 cm) was created aseptically by surgical incision. Then, animals were randomly allocated to four groups (I-untreated controls, II-topically treated with base gel, III-topically treated with 5% gel, IV-topically treated with 10% gel). Results:The results suggest that topical application of Pistacia atlantica oil gels improved reepithelialization with continuous stratum basalis and a mature granulation tissue and adnexa (hair follicles and sweat gland) compared with control and base gel groups. Treatment with 10% oil gel significantly enhanced the tensile strength, ultimate stress, yield strength and stiffness in this group compared with the control and base gel groups at 21 days post injury. The collagen fibers showed a more organized pattern and the tissue alignment was greater as compared to the control and base gel-treated groups at the same stage. Conclusion:The results suggest that topical application of Pistacia atlantica oil gel improved the morphological, biochemical and biomechanical properties of experimentally-induced wound defects in rats.

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Restoration of Strength Despite Low Stress and Abnormal Imaging after Achilles Injury

Abstract: Normal peak loads 4 wk after injury were withstood by an enlarged tendon of lower stress. These findings support progressive physical loading 4 wk after an Achilles tendon injury. T1-weighted OD constituted a marker of tendon mechanical recovery.

Cited by 16 publications

References 36 publications

Fetal derived embryonic-like stem cells improve healing in a large animal flexor tendonitis model

Fetal derived embryonic-like stem cells improve healing in a large animal flexor tendonitis model

Novel Application of Theranekron® Enhanced the Structural and Functional Performance of the Tenotomized Tendon in Rabbits

Cutaneous Wound Healing after Topical Application of <i>Pistacia atlantica</i> Gel Formulation in Rats

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