Evaluation of the influence of chemical agents before applying Self-etching Adhesives on dentin Bond Strength With the Self-etching adhesives, acid-etching with phosphoric acid has to be dispensed in dentin. Than, other solutions have been proposed in order to promote the cavity cleaner and improvement in bonding effectiveness. The aim of this study was to investigate the microshear bond strength, with the influence of chemical solutions and time, in three adhesive systems used on bovine dentin. Two hundred and forty bovine incisors were included, and the surface was polished in series with silicon carbide paper, getting flattened dentin surface of 10 mm in diameter. The specimens were randomly divided into four groups, according to the applied solution: SF-saline solution with 0.9% NaCl (control group), HS-deproteinization with sodium hypochlorite at 5%, for 2 minutes and HSAA-sodium hypochlorite 5% for 2 minutes followed by the application of ascorbic acid (ascorbate) and 10% for 1 minute;CHXapplication of chlorhexidine solution (2% for 1 minute). Were applied to surfaces prepared three adhesive systems, per group-Optibond All-in-One (Kerr), Filtek-P90 System Adhesive (3M-ESPE) and Adper SE (3M-ESPE), all followed by the insertion of composite resin (Filtek Z250 and Filtek P90). Half of the specimens of each group / treatment were stored for 24 hours and half for six months. After these periods were tested using the microshear in a universal testing machine (Emic, the speed of 0.5 mm / min). Results were submitted to three way ANOVA and TUKEY test multiple comparison both considering α = 0.05. The adhesive Filtek P90 System Adhesive was the one who kept the bond strength after aging, however, the two surface treatments containing sodium hypochlorite influenced negatively. With chlorhexidine, there was no statistically significant difference compared to the control group. The adhesive OptiBond All-in-One (Kerr) was affected by the sodium hypochlorite, sodium hypochlorite more ascorbate, at 24 hours. As a result of iqual with aging in aqueous solution. Adper SE (3M-ESPE) was influenced by the treatments using sodium hypochlorite. The best result, comparing two treatments with hypochlorite, after aging, was obtained by treatment with sodium hypochlorite more ascorbate was statistically identical to the control group. It was concluded that the influence of surface treatments on bond strength of adhesives tested. The chlorhexidine resulted in better results in almost every test.