Restriction endonuclease variation in the region of the alcohol dehydrogenase gene: a comparison of null and normal alleles from natural populations of Drosophila melanogaster

Jiang, Chengshan; Gibson, J. B.; Wilks, Ann Verona; Freeth, Allan L.

doi:10.1038/hdy.1988.15

Cited by 14 publications

(15 citation statements)

References 27 publications

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“…These duplications are now fixed in natural populations and derive from ancient mutational events. In contrast, the duplications reported at the Adh locus (Chia et al, 1985;Jiang et al, 1988) probably represent rare mutational events, making it difficult to ascertain their functional significance.…”

Section: Introductionmentioning

confidence: 80%

“…Compared with studies of restriction endonuclease variation at other loci in D. melanogaster [Adh (Birley, 1984;Aquadro et al, 1986;Cross and Birley, 1986;Jiang et al, 1988;Jiang 1989),Amy , Est6 (Game, 1989), Notch (Schaeffer et al, 1988), rosy , white (Langley and Aquadro, 1987;Miyashita and Langley, 1988), and Zest-tko (Aquade et al, 1989)], the values ofp, e, and h for the Gpdh region fall at the lower end of the range for each of these statistics. Three insertions were detected within the Gpdh gene region including a single large insertion.…”

Section: Discussionmentioning

confidence: 95%

“…Seven of these enzymes were used by Takano et al (1989) to reveal variation in the Gpdh region in two Japanese populations. Electrophoresis of the digests, transfer to nitrocellulose and hybridization conditions were as described by Jiang et al (1988). The probes pDm6Oa(c) and pDm59 (Cook et al, 1986) were used to detect fragments bearing Gpdh sequences and were labeled with [o~-32p]dCTP by nick translation (Rigby et al, 1977).…”

Section: Methodsmentioning

confidence: 99%

“…For example, in Drosophila melanogaster molecular evidence for duplications has recently been reported at the alcohol dehydrogenase (Adh) (Chia et al, 1985;Jiang et al, 1988), esterase-6 (Est6) (Collet et al, 1990), metallothionein (Mtn) (Otto et al, 1986), and white (w) (Davis et al, 1987) gene loci.…”

Section: Introductionmentioning

confidence: 98%

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Restriction site variation, gene duplication, and the activity ofsn-glycerol-3-phosphate dehydrogenase inDrosophila melanogaster

Symonds

Gibson

1992

Biochem Genet

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Restriction site variation in a 25-kb region including the sn-glycerol-3-phosphate dehydrogenase (Gpdh) locus has been assessed in 29 single female D. melanogaster lines from the Cardwell (Australia, QLD) population. The Gpdh locus was duplicated in about one-third of the lines, although the duplication was incomplete and lacked exons 1 and 2. There was no restriction site variation in the duplicated region. Three insertions were found in the gene region but only one affected GPDH activity. The lines with the duplication had higher levels of GPDH activity and protein amount than did nonduplicated lines. This effect was also observed in lines extracted from two other Australian populations. The duplication is shown to have a similar structure in each population investigated and is also present in populations from China and Africa. It is suggested that the effect of the duplication on GPDH activity, which might be due to structural factors affecting transcription at the Gpdh locus, could account for the worldwide distribution of the duplication.

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Section: Introductionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Restriction site variation, gene duplication, and the activity ofsn-glycerol-3-phosphate dehydrogenase inDrosophila melanogaster

Symonds

Gibson

1992

Biochem Genet

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“…Analyses of a number of Adh null alleles extracted from the Tasmanian populations showed that they all lacked ADH CRM (Freeth etal., 1986;1987), and probably shared a common origin (Jiang et at., 1988;Gibson & Wilks, 1989).…”

Section: Introductionmentioning

confidence: 99%

Low activity sn-glycerol-3-phosphate dehydrogenase variants in natural populations of Drosophila melanogaster

et al. 1991

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Eleven Australian natural populations of Drosophila melanogaster were screened by electrophoresis for evidence of null and/or low activity variants at the Gdph locus. Of 5018 alleles investigated, 57 had markedly lower GPDH activity, as judged by their heterozygous phenotypes, than control alleles. GPDH assays of 13 of these variant alleles showed that whilst they were heterogeneous in their properties and included electrophoretic variants and two categories of low-activity variant, there were no true null alleles. The most common low-activity variants exhibited dominance for GPDH activity in heterozygotes with normal alleles, and were shown to share this property with an allele previously isolated from a London (UK) population (Langley etal., 1981).

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Fatigue limit and crack arrest in alkali-containing silicate glasses

Gehrke¹,

Ullner²,

H�hnert³

1991

J Mater Sci

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Restriction endonuclease variation in the region of the alcohol dehydrogenase gene: a comparison of null and normal alleles from natural populations of Drosophila melanogaster

Cited by 14 publications

References 27 publications

Restriction site variation, gene duplication, and the activity ofsn-glycerol-3-phosphate dehydrogenase inDrosophila melanogaster

Restriction site variation, gene duplication, and the activity ofsn-glycerol-3-phosphate dehydrogenase inDrosophila melanogaster

Low activity sn-glycerol-3-phosphate dehydrogenase variants in natural populations of Drosophila melanogaster

Fatigue limit and crack arrest in alkali-containing silicate glasses

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