Restriction Fragment Fingerprint and Genome Sizes of<i>Staphylococcus</i>Species Using Pulsed-Field Gel Electrophoresis and Infrequent Cleaving Enzymes

Poddar, Saibal K.; McClelland, Michael

doi:10.1089/dna.1991.10.663

Cited by 10 publications

(5 citation statements)

References 27 publications

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“…Although there are biochemical and serological methods for species-specific discrimination among the staphylococci, methods for strain specific identification are needed for the accurate tracking of the epidemics and for conducting traceback studies. The molecular typing methods used to characterize staphylococci include ribotyping [15] and PFGE [13,16]. We used our modification of a PFGE method [13] to examine the genomic relatedness of 15 outbreak-associated isolates, which represented patient and food sources from both states involved.…”

Section: Discussionmentioning

confidence: 99%

Application of pulsed-field gel electrophoresis to the epidemiological characterization ofStaphylococcus intermediusimplicated in a food-related outbreak

1994

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An outbreak of food intoxication involving over 265 cases in western United States occurred in October 1991. Staphylococcus intermedius was implicated as the aetiologic agent. Representative outbreak isolates (five clinical and ten from foods) produced type A enterotoxin. DNA fragments generated by four restriction endonucleases and analysed by pulsed-field gel electrophoresis (PFGE) provided definitive evidence that all isolates from nine different counties in California and Nevada were derived from a single strain. The PFGE pattern of these outbreak isolates was distinct from those of a heterogeneous collection of seven S. intermedius strains of veterinary origin and five unrelated S. aureus laboratory strains. The data show a significant PFGE pattern heterogeneity not only among members of different Staphylococcus species but also within members of the same species and even the same enterotoxin type. The results indicate that PFGE is a valuable strain-specific discriminator for the epidemiological characterization of S. intermedius. To our knowledge, this represents the first documented foodborne outbreak caused by S. intermedius. These findings suggest that the presence of S. intermedius and other species such as S. hyicus in food should be reason for concern.

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Section: Discussionmentioning

confidence: 99%

Application of pulsed-field gel electrophoresis to the epidemiological characterization ofStaphylococcus intermediusimplicated in a food-related outbreak

1994

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“…[8][9][10][11][12] Ichiyama et al identified 3 1 restriction patterns amongst 1 1 1 isolates of methicillin-resistant S. aureus by their SmaI digestion pattern^.^ In this study the changes in the types of S . aureus colonising the human nasal cavity were followed for > 1 year.…”

Section: Introductionmentioning

confidence: 99%

Typing of Staphylococcus aureus colonising human nasal carriers by pulsed-field gel electrophoresis

Umeda

Kondo³

et al. 1995

Journal of Medical Microbiology

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Summary.Colonisation by Staphylococcus aureus in the nares of 120 outpatients and 63 healthy adults was studied for c. 2 years. Two states of carriage of S. aureus were confirmed : persistent carriage and persistent non-carriage. The states of carriage and non-carriage were quite stable and > 60 YO of the population of any of the study groups were stable non-carriers.The results of typing the strains isolated from the same individuals at different times with DNA fingerprinting by digestion with SmaI enzyme showed that all the stable carriers were persistently infected with the same strain and that changes in the strain seldom occurred.

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“…14 Increased resistance or susceptibility to several common poultry diseases, including tibial dyschondroplasia, Marek's disease, Rous sarcoma, and cecal coccidiosis, has been unequivocally linked to certain MHC genotypes. 9, 12, 16, 23, 25 In addition, MHC associations with moderately and highly pathogenic strains of S. aureus have been described in White Leghorn chickens. 8 Albeit, staphylococcosis and MHC associations have not been established in broiler breeder chickens.…”

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The Avian Major Histocompatibility Complex Influences Bacterial Skeletal Disease in Broiler Breeder Chickens

et al. 2005

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Abstract. This study evaluated bacterial skeletal disease in conjunction with the major histocompatibility complex (MHC) in a genetically pure line of broiler breeder chickens. Chickens from six broiler breeder flocks were examined for skeletal lesions, bacterial pathogens, and MHC genotype. During a 10-week period, eightyeight, 9-to 21-week-old lame chickens and 34 normal, age-matched controls were selected. Tenosynovitis, arthritis, and femoral or tibiotarsal (or both) osteomyelitis occurred in 86 of 88 (97.7%) lame chickens. Ninetyfive bacterial isolates were obtained from 83 of 88 (94.3%) lame birds and 4 of 34 (11.8%) controls. Staphylococcus spp. was isolated from 72.6% of the skeletal lesions, predominantly Staphylococcus aureus (38.9%). MHC B complex genotypes were determined by hemagglutination for 88 lame birds, 34 controls, and 200 randomly selected birds from each of the six flocks (1,200 total). Combined chi-square analysis revealed that the homozygous MHC genotypes B A4/A4 ( 2 ϭ 14.54, P ϭ 0.0063) and B A12/A12 ( 2 ϭ 42.77, P ϭ 0.0001) were overrepresented in the sample of symptomatic birds compared with random samples from the same flocks. The homozygous A4 and A12 MHC genotypes influenced flock chi-square values more than the corresponding heterozygotes. An MHC B complex influence on bacterial skeletal disease was apparent in this line of broiler breeders.

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Restriction Fragment Fingerprint and Genome Sizes ofStaphylococcusSpecies Using Pulsed-Field Gel Electrophoresis and Infrequent Cleaving Enzymes

Cited by 10 publications

References 27 publications

Application of pulsed-field gel electrophoresis to the epidemiological characterization ofStaphylococcus intermediusimplicated in a food-related outbreak

Application of pulsed-field gel electrophoresis to the epidemiological characterization ofStaphylococcus intermediusimplicated in a food-related outbreak

Typing of Staphylococcus aureus colonising human nasal carriers by pulsed-field gel electrophoresis

The Avian Major Histocompatibility Complex Influences Bacterial Skeletal Disease in Broiler Breeder Chickens

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