Restriction fragment length polymorphism analysis of the flaA gene of Campylobacter jejuni for subtyping human, animal and poultry isolates

Owen, R

doi:10.1016/s0378-1097(99)00258-x

Cited by 14 publications

(16 citation statements)

References 16 publications

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“…With multilocus enzyme electrophoresis analysis, a suitable method for evaluating population genetics of other bacteria, no subgroups of human and animal C. jejuni strains were identified (1). With other typing methods, such as PCR-RFLP analysis of flaA genes, considerable allelic variation of this gene in human and animal strains was observed (20). Also, RAPD analysis could not identify distinct subgroups (13).…”

Section: Discussionmentioning

confidence: 98%

Amplified Fragment Length Polymorphism Analysis of Campylobacter jejuni Strains Isolated from Chickens and from Patients with Gastroenteritis or Guillain-Barré or Miller Fisher Syndrome

Duim¹,

Ang²,

Belkum

et al. 2000

Appl Environ Microbiol

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The high-resolution genotyping method of amplified fragment length polymorphism (AFLP) analysis was used to study the genetic relationships between Campylobacter jejuni strains infecting chickens (n ‫؍‬ 54) and those causing gastroenteritis in humans (n ‫؍‬ 53). In addition, C. jejuni strains associated with the development of Guillain-Barré syndrome (GBS) (n ‫؍‬ 14) and Miller Fisher syndrome (MFS) (n ‫؍‬ 4), two related acute paralytic syndromes in human, were included. Strains were isolated between 1989 and 1998 in The Netherlands. The AFLP banding patterns were analyzed with correlation-based and band-based similarity coefficients and UPGMA (unweighted pair group method using average linkages) cluster analysis. All C. jejuni strains showed highly heterogeneous fingerprints, and no fingerprints exclusive for chicken strains or for human strains were obtained. All strains were separated in two distinct genetic groups. In group A the percentage of human strains was significantly higher and may be an indication that genotypes of this group are more frequently associated with human diseases. We conclude that C. jejuni from chickens cannot be distinguished from human strains and that GBS or MFS related strains do not belong to a distinct genetic group.

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Section: Discussionmentioning

confidence: 98%

Amplified Fragment Length Polymorphism Analysis of Campylobacter jejuni Strains Isolated from Chickens and from Patients with Gastroenteritis or Guillain-Barré or Miller Fisher Syndrome

Duim¹,

Ang²,

Belkum

et al. 2000

Appl Environ Microbiol

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“…Pulsed-field gel electrophoresis using three different restriction enzymes also identified clones found in cattle, poultry, and humans (19). However, restriction fragment length polymorphism analysis of the flaA gene using EcoRI and PstI showed that most flaA types were associated with several hosts (20).…”

Section: Discussionmentioning

confidence: 99%

Fluorescent Amplified Fragment Length Polymorphism Genotyping of Campylobacter jejuni and Campylobacter coli Strains and Its Relationship with Host Specificity, Serotyping, and Phage Typing

Hopkins¹,

Desai²,

Frost³

et al. 2004

J Clin Microbiol

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Fluorescent amplified fragment length polymorphism (FAFLP) analysis was applied to 276Campylobacter jejuni strains and 87 Campylobacter coli strains isolated from humans, pigs, cattle, poultry, and retail meats to investigate whether certain FAFLP genotypes of C. jejuni and C. coli are associated with a particular host and to determine the degree of association between FAFLP-defined genotypes and heat-stable serotypes and/or phage types. Within C. coli, the poultry strains clustered separately from those of porcine origin. In contrast, no evidence of host specificity was detected among C. jejuni strains. While C. coli strains show host specificity by FAFLP genotyping, C. jejuni strains that are genotypically similar appear to colonize a range of hosts, rather than being host adapted. Some serotypes and/or phage types (C. jejuni serotype HS18, phage type PT6, and serophage type HS19/PT2 and C. coli HS66, PT2, and HS56/PT2) were the most homogeneous by FAFLP genotyping, while others were more heterogeneous (C. jejuni HS5 and PT39, and C. coli HS24 and PT44) and therefore poor indicators of genetic relatedness between strains. The lack of host specificity in C. jejuni suggests that tracing the source of infection during epidemiological investigations will continue to be difficult. The lack of congruence between some serotypes and/or phage types and FAFLP genotype underlines the need for phenotypic testing to be supplemented by genotyping. This study also demonstrates how, in general, FAFLP generates "anonymous" genetic markers for strain characterization and epidemiological investigation of Campylobacter in the food chain.

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“…Studying the transmission of antimicrobial resistance from animals to man is difficult, especially from poultry to man, because the chain of transmission is often complex. However, several studies have shown that food animals can serve as an important source of infection in humans and that the same sero-and genotypes can be isolated from humans and food animals [37,38,40]. Since human-to-human transmission of C. jejuni/coli is rare, patients infected with resistant Campylobacter are not an important source of resistant Campylobacter for other humans.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance of thermophilic Campylobacter

Aarestrup¹,

2001

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-Campylobacter has become the leading cause of zoonotic enteric infections in developed and developing countries world-wide. Antimicrobial resistance has emerged among Campylobacter mainly as a consequence of the use of antimicrobial agents in food animal production. Resistance to drugs of choice for the treatment of infections, macrolides and fluoroquinolones has emerged as a clinical problem and interventions to reduce this are recommended. Resistance to fluoroquinolones and macrolides is mediated by chromosomal mutations. Resistance to other relevant antimicrobial agents, mediated by acquired resistance genes, has not become widespread so far. However, resistance genes originating from both Gram-positive and Gram-negative bacterial species have been found, showing the potential for acquired resistance to emerge in Campylobacter.Campylobacter / resistance / susceptibility testing / trends / gene Résumé -Résistance aux antimicrobiens chez Campylobacter thermophile. Campylobacter est devenu la cause principale de zoonoses entériques infectieuses dans les pays développés et en voie de développement à travers le monde. La résistance aux antibiotiques a émergé chez Campylobacter, principalement à cause de l'utilisation d'antibiotiques chez les animaux entrant dans la chaîne alimentaire. La résistance aux antibiotiques principalement utilisés dans le traitement des infections, les macrolides et les fluoroquinolones, a émergé en tant que problème clinique, et les interventions visant à réduire cette résistance sont recommandées. La résistance aux fluoroquinolones et aux macrolides est due à des mutations chromosomiques. La résistance aux autres antibiotiques utilisables, qui est due à l'acquisition de gènes de résistance, ne s'est jusqu'à maintenant pas propagée. Cependant, le fait qu'il ait été trouvé des gènes de résistance ayant pour origine des espèces bactériennes à Gram-positif et à Gram-négatif, montre la possibilité d'émergence de résistance acquise chez Campylobacter.Campylobacter / résistance / test de sensibilité / tendance / gène Vet. Res. 32 (2001) 311-321 311

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Restriction fragment length polymorphism analysis of the flaA gene of Campylobacter jejuni for subtyping human, animal and poultry isolates

Cited by 14 publications

References 16 publications

Amplified Fragment Length Polymorphism Analysis of Campylobacter jejuni Strains Isolated from Chickens and from Patients with Gastroenteritis or Guillain-Barré or Miller Fisher Syndrome

Amplified Fragment Length Polymorphism Analysis of Campylobacter jejuni Strains Isolated from Chickens and from Patients with Gastroenteritis or Guillain-Barré or Miller Fisher Syndrome

Fluorescent Amplified Fragment Length Polymorphism Genotyping of Campylobacter jejuni and Campylobacter coli Strains and Its Relationship with Host Specificity, Serotyping, and Phage Typing

Antimicrobial resistance of thermophilic Campylobacter

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