Restriction fragment length polymorphism of L1 amplicon using Rsa 1 detects five different human papillomavirus types and their co-infections among women attending a gynaecological outpatient department

Naqvi, Samar Husain; Wajid, Saima; Mitra, Amlan Kumar

doi:10.1016/j.jviromet.2003.12.002

Cited by 18 publications

(26 citation statements)

References 24 publications

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“…This range of HPV identification largely exceeds the number of types characterized by commercial assays currently available or by any other genotyping methodology based on RFLP analysis (Bernard et al, 1994;Pizzighella et al, 1995;Patti et al, 2002;Naqvi et al, 2004;Santiago et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…PCR using consensus primers with subsequent restriction fragment length polymorphism analysis (PCR-RFLP) has been demonstrated to be a sensitive and a financially advantageous methodology to detect and characterize HPV-DNA in clinical specimens (Lungu et al, 1992). However, the PCR-RFLP techniques previously described have some limitations: (a) they do not allow the genotyping of all high-risk HPV types (Pizzighella et al, 1995;Patti et al, 2002;Naqvi et al, 2004); (b) they often make use of a large number of restriction enzymes (Bernard et al, 1994;Astori et al, 1997); (c) they are ineffective or time consuming with regards to RFLP-pattern analysis on polyacrylamide gels and to identification of given HPV types (Bernard et al, 1994;Astori et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Complete genotyping of mucosal human papillomavirus using a restriction fragment length polymorphism analysis and an original typing algorithm

Nobre

Almeida

Martins

2008

Journal of Clinical Virology

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Our polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, using 4 restriction enzymes (PstI, HaeIII, DdeI, RsaI) and an original genotyping algorithm, allows discrimination of all individual mucosal HPV types in single infections, and even detection of multiple infections. This assay gives complementary information to commercially available methods, and may also be financially advantageous, particularly when financial resources are scarce.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Complete genotyping of mucosal human papillomavirus using a restriction fragment length polymorphism analysis and an original typing algorithm

Nobre

Almeida

Martins

2008

Journal of Clinical Virology

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“…This method was also efficient at distinguishing HPV types 6, 11, 16, 18, and 33 and the co-infection of them. 14 The results demonstrated that the RsaI digestion of the MY-PCR products of CA lesions is a rapid and efficient method to type frequent HPV in China, which may have a strong potential for use in clinical diagnosis.…”

Section: Human Papillomavirus Typing By Rsai Digestion Of Mypolymerasmentioning

confidence: 93%

Correlation of human papillomavirus types with clinical features of patients with condyloma acuminatum in China

et al. 2015

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“…In this technique, the amplified DNA is digested by restriction enzymes, resulting in DNA fragments of various lengths. The commonest restriction enzymes are Bam HI, Dd6e I, Hae III, Hin fI, Pst I and Rsa I [42]. However, Santiago et al used a single restriction enzyme, Hpy CH4V, to detect 21 HR- and 31 LR-HPV genotypes [43].…”

Section: Introductionmentioning

confidence: 99%

A review of methods for detect human Papillomavirusinfection

Abreu

Souza

Gimenes

et al. 2012

Virol J

232

197

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Human Papillomavirus (HPV) is the most common sexually transmitted virus. Worldwide, the most common high-risk (HR)-HPV are -16/18, and approximately 70% of cervical cancers (CC) are due to infection by these genotypes. Persistent infection by HR-HPV is a necessary but not sufficient cause of this cancer, which develops over a long period through precursor lesions, which can be detected by cytological screening. Although this screening has decreased the incidence of CC, HPV-related cervical disease, including premalignant and malignant lesions, continues to be a major burden on health-care systems. Although not completely elucidated, the HPV-driven molecular mechanisms underlying the development of cervical lesions have provided a number of potential biomarkers for both diagnostic and prognostic use in the clinical management of women with HPV-related cervical disease, and these biomarkers can also be used to increase the positive predictive value of current screening methods. In addition, they can provide insights into the biology of HPV-induced cancer and thus lead to the development of nonsurgical therapies. Considering the importance of detecting HPV and related biomarkers, a variety of methods are being developed for these purposes. This review summarizes current knowledge of detection methods for HPV, and related biomarkers that can be used to discriminate lesions with a high risk of progression to CC.

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Restriction fragment length polymorphism of L1 amplicon using Rsa 1 detects five different human papillomavirus types and their co-infections among women attending a gynaecological outpatient department

Cited by 18 publications

References 24 publications

Complete genotyping of mucosal human papillomavirus using a restriction fragment length polymorphism analysis and an original typing algorithm

Complete genotyping of mucosal human papillomavirus using a restriction fragment length polymorphism analysis and an original typing algorithm

Correlation of human papillomavirus types with clinical features of patients with condyloma acuminatum in China

A review of methods for detect human Papillomavirusinfection

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