Restriction pattern of the major outer-membrane protein gene provides evidence for a homogeneous invasive group among ruminant isolates of Chlamydia psittaci

Abstract

Thirty-six ruminant isolates of Chlamydia psittaci, previously classified as invasive or non-invasive in a mouse model of virulence, were compared by analysing A h 1 restriction patterns of the major outer-membrane protein (MOMP) gene after DNA amplification by the polymerase chain reaction. The 24 invasive isolates, although from various origins, all belonged to serotype 1 and represented a strictly homogeneous group sharing a specific MOMP-gene restriction'pattern that was not observed in the non-invasive strains. On the other hand, the 12 noninvasive strains, although all belonging to serotype 2, constituted a heterogeneous group with eight distinct MOMP-gene restriction patterns. However, all eight patterns shared a 180 bp fragment or the corresponding restricted fragments of 110 and 70 bp. MOMP-gene restriction patterns also clearly distinguished the ruminant strains from an avian C.psi#uciisolate, a Cpneumoniae isolate and two C. truchomatis isolates which were studied for comparison. The homogeneous character of the invasive C. psiffuci strains argues strongly for their genetic relatedness. Our results illustrate the usefulness of the MOMP-gene restriction mapping in typing chlamydiae.

Conjunctivitis Caused by a SwineChlamydia Trachomatis-Like Organism in Gnotobiotic Pigs

“…9,10 Chlamydiae were analyzed by PCR amplification of the major outer membrane protein genome using the primers and basic techniques reported previously. 3 The PCR product was verified by electrophoresis in 1.5% agarose. The product was then digested with the Alu I restriction endonuclease for RFLP analysis.…”

Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms

“…Rodolakis, personal communication), but are serologically and pathogenically diverse. Chlamydophila pecorum strains characterized thus far have been limited to mammals, but not to a specific host family: Chlamydophila pecorum has been isolated from ruminants (cattle, sheep and goats) (Denamur et al, 1991;Fukushi & Hirai, 1992), a marsupial (koala) (Girjes et al, 1993;Glassick et al, 1996;M. Jackson et al, 1997) and swine (Anderson et al, 1996;Everett & Andersen, 1997;Kaltenboeck & Storz, 1992).…”

“…Other monoclonal serotyping systems have also been developed (Fukushi et al, 1987;Takahashi et al, 1988Takahashi et al, , 1997, but a comparative study of these methods has not been done. Chlamydophila abortus strains are endemic among ruminants and efficiently colonize the placenta (Rodolakis et al, 1989 (Andersen, 1991 ;Denamur et al, 1991 ;Storz et al, 1960). Chlamydophila abortus isolates have also been characterized by serology and sequence analysis from cases of abortion in a horse, a rabbit, guinea pigs and mice (J. Storz, K. D. E. Everett, A.…”

Protection evaluation against Chlamydophila abortus challenge by DNA vaccination with a dnaK-encoding plasmid in pregnant and non-pregnant mice

“…After lysis of C. abortus bacteria by treatment with proteinase K (2 mg/mL) and L-laurylsarcosine (2%), genomic DNA of C. abortus was extracted as previously described [12]. The genomic library of C. abortus DNA was constructed with the LambdaGEM®12 kit (Promega Corporation, Madison, WI, USA) and according to the recommendations of the manufacturer.…”