Result of attempted hematopoietic reconstitution using isologous, peripheral blood mononuclear cells: a case report

Abstract

In order to test the effect of peripheral blood mononuclear cell infusions on hematopoietic recovery in man we intensively leukapheresed a normal identical twin and obtained 9.8 x 10(10) peripheral blood mononuclear cells containing 4 x 10(5) CFU-C. These isologous cells were infused into his identical twin brother who had received 150 rad of total body irradiation and intensive combination chemotherapy as adjuvant therapy for Ewing's sarcoma. When compared to other patients receiving similar treatment, leukocyte recovery was accelerated by 3–4 wk, and occurred at a rate comparable to that induced by infusion of autologous cryopreserved marrow. Recovery of granulocytes, monocytes and platelets was not accelerated. The low number of CFU-C present in the preparation used ((one-eighth the number of CFU-C we usually obtain from bone marrow autograft collections) may have led to the pattern of hematopoietic recovery we observed in this patient.

Hematopoietic potential of stem cells isolated from murine skeletal muscle

“…Adult muscle is highly vascularized and contains a variety of mononuclear cells, including those in peripheral blood, as well as endothelial cells, fibroblasts, and myogenic satellite cells. Because the prevalence of HSC in peripheral blood is at least 100-fold lower than that in the bone marrow (35)(36)(37) and because other highly vascularized tissues (kidney and liver) containing endothelial cells and fibroblasts do not display high levels of HSC activity (data not shown), we speculate that myogenic satellite cells are the most likely candidates for this role.…”

Criopreservação de medula óssea e células pluripotentes periféricas utilizando um congelador programável: experiência em 86 congelamentos

“…O mesmo fenômeno foi observado durante o tratamento de indução na leucemia mielóide aguda e, após quimioterapia, para linfoma não-Hodgkin. Abrams et al demonstraram um efeito semelhante em cães, que receberam ciclofosfamida 10 . Esses autores notaram que a coleta de células, após estímulo com o quimioterápico, resultou em número 12 vezes maior do que o obtido em animais-controle, sem estímulo.…”

A Review of Advances in Hematopoietic Stem Cell Mobilization and the Potential Role of Notch2 Blockade

“…Fortuitously, around the same time, a continuous flow centrifuge (apheresis) machine was developed to improve harvesting enough peripheral repopulating units for transplantation in patients with chronic myelocytic leukemia 8,9 . Nevertheless, several subsequent attempts to transfuse peripheral white blood cells among identical twins did not give a plausible engraftment result 8,10 . Putatively, the reason for the failure was due to the insufficient number of hematopoietic stem and progenitor cells (HSPCs) present in the peripheral blood compared to those in the BM.…”