Résultats Obtenus Au Cours De l'ÉTUDE De Quelques Facteurs Importants De l'INSÉMINATION Artificielle Ovine

Colas, G.; Dauzier, L.; Courot, M.; Ortavant, R.; Signoret, J.-P.

doi:10.1051/animres:19680104

Cited by 38 publications

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“…More recently, Mara et al (2005) found an improvement in fertility and blastocyst rate development in an in vitro experiment, when sperm were stored at 15 • C in presence of the antioxidant TEMPOL, and Casao et al (2010) reported an increase in the cleavage rate after treating sperm with small amounts of melatonin. In addition to the effects of antioxidants, there was an overall greater quality in the samples of the present study that were maintained at 15 • C. Although there are many reports on the quality of ram semen stored either at 15 or 5 • C (Salamon and Maxwell, 2000), only a few of these previous studies directly compared both temperatures under similar conditions (Colas et al, 1968;Hollinshead et al, 2004;O'Hara et al, 2010). These direct-comparison studies are necessary to fully understand differences between these two temperatures while controlling for confounding variables, and thus this was one of the objectives of the present study.…”

Section: Discussionmentioning

confidence: 62%

“…Indeed, in previous studies when INRA96 was used, as was the case in the present study, the differences in sperm quality for the different temperatures were much less at 48 h of storage. Colas et al (1968) also detected small differences when comparing sperm quality after 2 and 14 h (milk-catalase extender) of storage, whereas Hollinshead et al (2004) stored the samples up to 24 hand found no differences.…”

Section: Discussionmentioning

confidence: 90%

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Refrigerated storage of ram sperm in presence of Trolox and GSH antioxidants: Effect of temperature, extender and storage time

Mata-Campuzano

Álvarez‐Rodríguez

Tamayo-Canul

et al. 2014

Animal Reproduction Science

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Antioxidants have a potential to improve the quality and fertility of refrigerated-stored ram semen. Reduced glutathione (GSH) and Trolox (0.2, 1 and 5mM) were evaluated in ram semen preserved at 15 and 5°C up to 48 and 96h, respectively. Extenders were also evaluated (15°C: Tris-citrate-fructose, TCF, without lipids, and TES-Tris-fructose 10% egg yolk, TTF-EY; 5°C: TTF-EY and 3.5% soybean lecithin, TTF-SL; INRA96 at both temperatures). Storage at 5°C resulted in poorer quality than 15°C up to 48h, while allowing acceptable quality at 96h. Antioxidants had few effects on sperm quality, with use of Trolox resulting in reduced motility and viability in TCF. Storage at 15°C in the TCF extender resulted in decreased motility, viability and mitochondrial activity compared with use of TTF-EY. Sperm quality when storage was at 5°C was similar, but storage in TTF-SL resulted in decreased motility and mitochondrial activity. Acrosomal status was only slightly affected by extender and antioxidant. Mitochondrial activity was improved by antioxidants in TTF-SL, and GSH at 5mM when semen was stored at 5°C in TTF-EY. A preliminary artificial insemination trial indicated that supplementation with GSH has the potential for improving lambing (P<0.1). In conclusion, use of antioxidants resulted in lesser effects than extender composition or storage time on quality of ram semen. Use of Trolox negatively impacted sperm quality and GSH had some positive impacts. The use of soybean lecithin requires further research to assess its impact on mitochondria.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 90%

Refrigerated storage of ram sperm in presence of Trolox and GSH antioxidants: Effect of temperature, extender and storage time

Mata-Campuzano

Álvarez‐Rodríguez

Tamayo-Canul

et al. 2014

Animal Reproduction Science

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“…Ejaculated spermatozoa were diluted into skimmed milk and brought to 15 °C (Colas et al, 1968). Epididymal spermatozoa were obtained from different segments of the epididymidis caput (distal), corpus (I = middle, 11 = inferior middle and III = distal part), and cauda (proximal and distal) according to Fournier-Delpech et al (1977).…”

Section: Methodsmentioning

confidence: 99%

Epididymal sperm maturation in the ram : motility, fertilizing ability and embryonic survival after uterine artificial insemination in the ewe

Fournier-Delpech¹,

Colas²,

Courot³

et al. 1979

Ann. Biol. anim. Bioch. Biophys.

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Summary. Ram spermatozoa from the distal caput, corpus, and cauda epididymis and ejaculated spermatozoa were artificially inseminated in 170 ewes which had been treated with an ovulatory dose of PMSG 61 ! 4 hrs previously. Results were expressed as the percent of ewes pregnant 18 days after insemination (F = with positive progesterone assay/ Q inseminated) and embryonic survival (ES = ewes lambing/ pregnant on day 18). F =0, 8, 12, 54, 78, 80 and 72 p. 100 respectively after insemination with 20 x 10 6 total spermatozoa/horn from the caput, corpus, I, II and III, proximal cauda, distal cauda, and ejaculate.Development of sperm fertilizing ability parallelled the development of sperm motility. However when ewes were inseminated with high number of motile spermatozoa, ES was 0, 77, 86, 100 and 86 p. 100 with spermatozoa from the corpus I --!-II, the corpus III, the proximal cauda, the distal cauda, and the ejaculate respectively. Thus, the sperm from middle corpus are totally unable to produce viable lambs.In the ram the development of sperm fertilizing ability in the epididymis coincides with the development of progressive motility (Fournier-Delpech et al., 1977) in a maturation process similar to the one described in rodents (reviews of OrgebinCrist et al.,1975OrgebinCrist et al., , 1976Bedford, 1975) or in the pig (Holtz and Smidt, 1976 ;Hunter et al., 1976). However, in the rabbit embryonic mortality is increased after fertilization by immature spermatozoa from the corpus epididymis (Orgebin-Crist, 1969 ; OrgebinCrist and Jahad, 1977). Similarly, litter size in rats is smaller after intra-uterine insemination with epididymal spermatozoa from the corpus than after insemination with spermatozoa from the cauda (Paz et al., 1978 (Colas, 1975 and1979 ;Gordon, 1975 a) After insemination with a constant number of spermatozoa (20 x 10 6 per horn) the pregnancy rate developed constantly which is correlated with the number of progressive motile cells inseminated per horn in the corpus (r = 0.9 in the middle corpus ; r = 0.6 in the distal corpus). However, the plateau is reached at a lower motile sperm concentration and the pregnancy rate is higher when more mature spermatozoa are inseminated ( fig. 2).Progressive motility may be the only essential factor to deliver the fertilizing agent to the oocyte for fertilization. However, development of motility is not definite proof of fertilizing ability for a potential fertile sperm. The pregnancy rate showed that fertilizing ability had not been completely achieved in the middle corpus (60 p. 100 with 20 X 10 6 motile spermatozoa inseminated/horn from the distal corpus versus 90 p. 100 with 8 x 10 6 motile spermatozoa from the distal corpus). Consequently, the acquisition of a mature motility pattern may not be sufficient for fertilization. b) A higher embryonic mortality after insemination with young epididymal spermatozoa has been reported in the rabbit (Orgebin-Crist, 1969) and the pig (Hunter et al., 1976), but was not observed by Overstreet and Bedford (1976) (Fournier...

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“…J. Anim. Sci.59: 685-691 (Dec. 1979) (Ortavant 1977;Robinson 1965Robinson , ,1967 concentration of 900 x 106/ml, using a skim milk-citrate diluent at 30"C (Colas et al 1968). The diluted semen was cooled slowly and maintained at 16"C for up to 12 h.…”

mentioning

confidence: 99%

A Comparison of Fresh and Frozen Semen in the Insemination of Confined Sheep

Langford¹,

Marcus²,

Hackett³

et al. 1979

Can. J. Anim. Sci.

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Résultats Obtenus Au Cours De l'ÉTUDE De Quelques Facteurs Importants De l'INSÉMINATION Artificielle Ovine

Cited by 38 publications

References 0 publications

Refrigerated storage of ram sperm in presence of Trolox and GSH antioxidants: Effect of temperature, extender and storage time

Refrigerated storage of ram sperm in presence of Trolox and GSH antioxidants: Effect of temperature, extender and storage time

Epididymal sperm maturation in the ram : motility, fertilizing ability and embryonic survival after uterine artificial insemination in the ewe

A Comparison of Fresh and Frozen Semen in the Insemination of Confined Sheep

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