Results of Application of the New Nutrient Media Set for Cholera Diagnostics within the Frames of the Special Tactical Training Exercises for Specialized Anti-Epidemic Team

Mazrukho, A B; Kaminsky, D. I.; Lomov, Yu. M.; Телесманич, Н Р; Rozhkov, K K; Кругликов, В. Д.; Goncharenko, E. V.; Тришина, А. В.; Pukhov, Yu. M.; Prometnoy, V. I.; Пичурина, Н. Л.

doi:10.21055/0370-1069-2011-3(109)-54-57

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Имеются данные литературы об использовании комплекса диагностических питательных сред на основе гидролизата пекарских дрожжей для выделения и накопления холерных вибрионов [5,7,8]. Однако в доступной нам литературе нет сведений о возможности применения сред на основе автолизата или гидролизата дрожжей при глубинном культивировании штаммов, используемых в производстве ХВ.…”

unclassified

Development of Food-Raw-Material-Based Nutrient Media for Submerged Cultivation of Cholera Vibrio Strains

Никифоров¹,

Антонычева²,

Volokh³

et al. 2015

Problemy Osobo Opasnykh Infektsii

View full text Add to dashboard Cite

Carried out has been comparative assessment between the performances of liquid nutrient pancreatic fibrin overcook-based and bakery yeast autolyzate-based media and conventionally used in manufacturing of cholera vaccine media for submerged cultivation of Vibrio cholerae 569B and V. cholerae M-41 strains. Results of investigation of media quality biological predictors (morphological and biochemical property stability, efficacy of biomass and protective antigen accumulation), which are fibrin hydrolyzate and bakery yeast autolyzate-based, suggest the possibility of using them for the production of cholera vaccine. Deployment of inedible raw material-based media in manufacturing of cholera vaccine is a prospective technology in view of reduction of medical-prophylactic preparation costs. Moreover it allows for solving the problem of protein waste-product disposal, which is generated in the process of anti-rabies immunoglobulin manufacturing, thus decreasing ecological impact on the environment.

show abstract

unclassified