Results of Serial Measurement of Estradiol in Serum with Six Different Methods during Ovarian Stimulation

Mikkelsen, Anne Lis; Borggaard, Birgit; Lebech, P. E.

doi:10.1159/000292032

Cited by 14 publications

(6 citation statements)

References 7 publications

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“…Blood was collected from either the aorta or the heart by needle aspiration, and the serum fractions were assayed for testosterone and estrogen levels. The hormonal concentrations were measured using a Spectria radio immunoassay kit (Orion Diagnostica, Espoo, Finland) for both testosterone and estrogen 12 …”

Section: Methodsmentioning

confidence: 99%

Effects of androgen deprivation on chronic bacterial prostatitis in a rat model

Seo¹,

Lee²,

Kim³

et al. 2003

Int J of Urology

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Background : Many attempts have been made to improve the treatment success rate of chronic bacterial prostatitis (CBP). However, no treatment modality has achieved complete cure. The growth and development of the prostate is under direct hormonal control, and it is possible that prostatitis may be directly influenced by its hormonal milieu in a similar fashion to benign prostatic hyperplasia and prostate cancer. Therefore, the effects of androgen deprivation on the treatment of CBP were investigated in rats. Methods : Experimental CBP was induced in one hundred, male Wistar rats by instillation of bacterial suspension ( Escherichia coli Z17, O2: K1: H-) containing 1 ¥ 10 8 CFU/ m L into the prostatic urethra. Microbiologically and histologically proven CBP was demonstrated in 62% (62 of 100) of the rats after 4 weeks of bacterial instillation. The 62 rats demonstrating CBP were randomly divided into five groups: control; castration; finasteride; estrogen; and levofloxacin groups. All drug treatments were conducted over a period of 4 weeks. Results : Microbiological cultures and histological findings of the prostate and urine samples demonstrated reduced bacterial growth and improved inflammatory responses in all four experimental groups compared with the control group. The castration and estrogen groups showed coherent trends of decrease in bacterial growth and improvements in prostatic inflammation compared with the control group, but not to a statistically significant degree ( P > 0.05). However, the finasteride and levofloxacin groups did show statistically significant decreases in bacterial growth and improvements in prostatic inflammation compared with the control group ( P < 0.05). Conclusion : These results suggest that androgen deprivation is an effective modality in CBP treatment. In particular, the finasteride treatment reduced the severity of CBP in the animal model without reducing the systemic testosterone level. The combination of finasteride and levofloxacin maybe one of the effective treatment modalities for CBP.

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Section: Methodsmentioning

confidence: 99%

Effects of androgen deprivation on chronic bacterial prostatitis in a rat model

Seo¹,

Lee²,

Kim³

et al. 2003

Int J of Urology

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“…The values obtained for serum E 2 depend on the method used (17)(18)(19)(20). Various factors may account for the differences between the two methods (21 ).…”

Section: Use Of Thementioning

confidence: 99%

Use of the Architect-i2000 Estradiol Immunoassay during in Vitro Fertilization

et al. 2003

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“…The tests were undertaken using an automated qualitative enzyme-linked¯uorescent assay developed on the Vidas analyser (bioMe Ârieux) [Weber and Doerr, 1993;Mikkelsen et al, 1996]. This two-step capture tag test was performed as follows: the antibodies to be tested for their ability to detect HBsAg variants were coated on the Solid Phase Receptacle (SPR) at the ®nal concentration of 10 mg/ml.…”

Section: Detection Of Hbsag Variants With Mabsmentioning

confidence: 99%

Localization of hepatitis B surface antigen epitopes present on variants and specifically recognised by anti‐hepatitis B surface antigen monoclonal antibodies

Jolivet-Reynaud¹,

Lesénéchal²,

O'Donnell³

et al. 2001

Journal of Medical Virology

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Small hepatitis B surface antigen (HBsAg) is considered to be the best marker for the diagnosis of Hepatitis B virus infection. However, HBsAg variants with mutations within the "a" determinant may be poorly or not detected by diagnostic assays. Three anti-HBsAg monoclonal antibodies (6H6B6, 27E7F10, and 2G2G10), directed against conformational epitopes, were tested for their ability to detect the wild-type HBsAg as well as variant forms and their respective epitopes were localised on the HBsAg sequence by using the phage-displayed peptide library technology. Whereas 6H6B6 did not detect mutations T123N, S143L, D144A and G145R, 27E7F10 binding was affected by mutations P120T and G145R. In contrast, 2G2G10 reacted strongly with all tested variants including variant with the G145R mutation. Part of the 6H6B6 epitope was located in the major hydrophilic region (MHR) at residues 101-105, the 27E7F10 epitope (residues 214-219) was located near the C-terminal end of the antigen and the 2G2G10 epitope at residues 199-208, within the theoretical fourth transmembrane helix. The 2G2G10 epitope localisation brings information about the HBsAg structure and the validity of established topological models. Finally, 2G2G10 is a valuable tool for HBsAg variant detection that is used as capture phase in a new bioMérieux diagnostic assay, which is currently in development.

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Results of Serial Measurement of Estradiol in Serum with Six Different Methods during Ovarian Stimulation

Cited by 14 publications

References 7 publications

Effects of androgen deprivation on chronic bacterial prostatitis in a rat model

Effects of androgen deprivation on chronic bacterial prostatitis in a rat model

Use of the Architect-i2000 Estradiol Immunoassay during in Vitro Fertilization

Localization of hepatitis B surface antigen epitopes present on variants and specifically recognised by anti‐hepatitis B surface antigen monoclonal antibodies

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