Resuscitation of Tenacibaculum sp., the causative bacterium of spotting disease of sea urchin Strongylocentroutus intermedius, from the viable but non-culturable state

Masuda, Yuuki; Tajima, Kenichi; Ezura, Yoshio

doi:10.1111/j.1444-2906.2003.00801.x

Cited by 18 publications

(12 citation statements)

References 18 publications

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“…Similar results have been described for a Tenacibaculum sp. strain by Masuda and colleagues (2004), which was recently designated as Flexibacter echinicida (Y. Masuda, pers. comm.).…”

Section: Discussionmentioning

confidence: 99%

Use of microcosms to determine the survival of the fish pathogen Tenacibaculum maritimum in seawater

Avendaño‐Herrera

Irgang

Magariños

et al. 2006

Environmental Microbiology

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The survival of the fish pathogen Tenacibaculum maritimum in different seawater microcosms was investigated during 160 days. The persistence of culturable cells was greater in sterile than in natural seawater. Standard plate counts showed that T. maritimum survived in sterile seawater for more than 5 months at concentration around 10(3) cfu ml(-1). However, T. maritimum proved to be very labile in non-sterile seawater, rendering culturable cells no longer than 5 days. These results were confirmed when DNA-based methods were applied. Regardless of the microcosms used, epifluorescence microscopy counts remained at about 10(6) cells ml(-1) throughout the experiment, even though we can not distinguish T. maritimum in the case of non-sterile microcosms. Resuscitation assays with addition of fresh medium to non-sterile microcosms did not favour the recovery of T. maritimum on solid media. Although morphological changes from filamentous to spheres were observed after 3 days in the non-sterile microcosms, in the case of the sterile microcosms this change was observed at the sixth day. The biochemical, physiological, serological and genetic characteristics were unaffected in the sterile microcosms. The overall results contribute to a better understanding of the behaviour of T. maritimum in natural seawater and suggest that the aquatic bacterial population play an important role in the survival of this fish pathogen.

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“…Similar results have been described for a Tenacibaculum sp. strain by Masuda and colleagues (2004), which was recently designated as Flexibacter echinicida (Y. Masuda, pers. comm.).…”

Section: Discussionmentioning

confidence: 99%

Use of microcosms to determine the survival of the fish pathogen Tenacibaculum maritimum in seawater

Avendaño‐Herrera

Irgang

Magariños

et al. 2006

Environmental Microbiology

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“…1). Strain B-I T was most closely related (99.5-99.7 %) to '[Flexibacter] echinicida', an unpublished bacterial species isolated from diseased sea urchin (Tajima et al, 1997a, b) and now considered as a member of Tenacibaculum (Masuda et al, 2004). Unfortunately, it has not been possible to obtain strains of '[Flexibacter] echinicida' for comparison with strain B-I T .…”

mentioning

confidence: 99%

Tenacibaculum litopenaei sp. nov., isolated from a shrimp mariculture pond

Sheu

Lin

Chou

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

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A yellow-coloured, aerobic, Gram-negative, rod-shaped bacterial strain, designated B-I T , was isolated from the water of a shrimp (Litopenaeus vannamei) mariculture pond in Taiwan. No species with a validly published name showed 16S rRNA gene sequence similarity of more than 96.5 % to this novel isolate. The highest sequence similarities displayed by strain B-I T (93.2-96.1 %) were to members of the genus Tenacibaculum. The phenotypic properties of this organism were consistent with its classification in the genus Tenacibaculum. The novel isolate could be distinguished from all Tenacibaculum species by several phenotypic characteristics. The major fatty acids were iso-C 15 : 0 (22 %), summed feature 3 (C 16 : 1 v7c and/or iso-C 15 : 0 2-OH; 21.3 %), iso-C 17 : 0 3-OH (12.7 %) and iso-C 15 : 1 (8.7 %). The G+C content of the genomic DNA was 35.2 mol%. Hence, genotypic and phenotypic data demonstrate that strain B-I T should be classified within a novel species in the genus Tenacibaculum, for which the name Tenacibaculum litopenaei sp. nov. is proposed. The type strain is B-I T (=BCRC 17590 T =LMG 23706 T ).

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“…At the same time sample C incubated in rich optimal environment of nutrient broth had the highest culturability for the first three months. These differences caused by environmental conditioning in our opinion shed some light on question why there is a strain difference in speed and sometimes even possibility of entry into nonculturable state (Chaveerach P., 2003, Masuda Y., 2004.…”

Section: Discussionmentioning

confidence: 75%

“…Also reversion of nonculturable cells was achieved using different supplements and conditions. 3,93±0,35×10 7 3,82±0,3×10 7 3,86±0,27×10 7 3,7±0,31×10 7 3,75±0,31×10 7 1 3,25±0,29×10 7 2,82±0,25×10 7 2,52±0,18×10 7 2,87±0,24×10 7 3,29±0,28×10 7 2 3,06±0,27×10 7 1,75±0,14×10 7 8,8±0,8×10 6 2,2±0,18×10 7 2,33±0,19×10 7 7 1,17±0,1×10 7 3,06±0,21×10 6 1,98±0,15×10 6 2,75±0,23×10 6 4,1±0,34×10 6 15 4,21±0,37×10 6 1,54±0,12×10 6 1,18±0,09×10 6 2,52±0,21×10 6 3,64±0,3×10 5 20 9,25±0,83×10 5 2,4±0,19×10 5 5,58±0,44×10 5 1,83±0,15×10 6 9,26±0,76×10 4 41 6,05±0,54×10 5 1,2±0,09×10 4 1,57±0,12×10 5 6,75±0,56×10 4 5,39±0,44×10 4 69 7,86±0,7×10 5 0 5,38±0,43×10 5 6,97±0,57×10 5 3,13±0,26×10 4 85 6,08±0,54×10 5 2,4±0,16×10 1 3,8±0,28×10 5 5,5±0,47×10 4 2,08±0,17×10 5 119 5,8±0,46×10 5 3,48±0,26×10 4 6,7±0,49×10 5 3,45±0,3×10 5 1,28±0,1×10 5 148 1,15±0,09×10 5 8,76±0,52×10 3 7,72±0,64×10 5 8±0,69×10 4 8,6±0,72×10 4 162 1,56±0,12×10 5 9,96±0,79×10 3 1,24±0,1×10 6 1,5±0,13×...…”

Section: Resultsunclassified

Formation and Reversion of VBNC Cells of Salmonella Typhimurium Preincubated in Different Substrates

2017

IJCRR

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Objectives: Salmonella is one of the main dangerous opportunistic microorganisms, and it can persist in viable but nonculturable (VBNC) state in different foodstuffs, water, human or animal organisms etc. Thus it is important to know more about this form of bacterial existence. Methodology: In this study Salmonella Typhimurium 79 strain was used, in which RP4 plasmid was introduced. Prior to entering nonculturability inducing conditions cells of the strain were preincubated in one of the substrates that are typical sources of Salmonella contamination. Several additives and conditions were evaluated in order to revert nonculturable Salmonella into vegetative state. Results: Influence of preincubation in different substrates on speed and totality of entry into nonculturable state was determined. It was shown that preincubation in soil had the greatest impact on the Salmonella population. Control population, preincubated in nutrient broth, was the slowest to enter nonculturable state. Tested reversion factors had effects at different concentrations. The most efficient, though the slowest, way to revert Salmonella was to incubate VBNC cells in 0.9% NaCl. Conclusion: Obtained data reveal some reasons for strain differences in speed of formation of VBNC cells. Reversion was achieved using several supplements and conditions.

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Resuscitation of Tenacibaculum sp., the causative bacterium of spotting disease of sea urchin Strongylocentroutus intermedius, from the viable but non-culturable state

Cited by 18 publications

References 18 publications

Use of microcosms to determine the survival of the fish pathogen Tenacibaculum maritimum in seawater

Use of microcosms to determine the survival of the fish pathogen Tenacibaculum maritimum in seawater

Tenacibaculum litopenaei sp. nov., isolated from a shrimp mariculture pond

Formation and Reversion of VBNC Cells of Salmonella Typhimurium Preincubated in Different Substrates

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