Tenacibaculum sp., the causative bacterium of spotting disease of sea urchin Strongylocentroutus intermedius , has been known to enter into the viable but non-culturable (VBNC) state in 75% artificial seawater (ASW) at 5 ∞ C. From this fact it is suspected that this bacterium probably resuscitates from the VBNC state in summer. To examine whether non-culturable cells of Tenacibaculum sp. are able to regain the culturable state, VBNC cells in 75% ASW and 75% ASW containing homogenated sea urchin (HSU) were treated with temperature upshift (5 ∞ C to 25 ∞ C) and addition of iron chloride. Resuscitation from VBNC cells with temperature upshift was observed only in 75% ASW containing HSU for 3 days incubation after entry into the VBNC state. Resuscitation of VBNC cells treated with iron chloride was observed in each microcosm. Resuscitation from the VBNC state was confirmed by dilution experiments and measurements of generation time. Resuscitated cells regained original characteristics, agglutination against anti-Tenacibaculum sp. strain F-2, pathogenicity to sea urchin and morphology.
The + / − ratio originating from string decays is predicted to be >1 in pp interaction at SPS energies. The anti-dominance increases with decreasing beam energy. This surprising behaviour is caused by the combinatorics of quark-antiquark production in small, finite strings. Since this behaviour is not found in a statistical description of hadron production in pp collisions, it may serve as a potent observable to probe the hadronization mechanism in such collisions.* These proceedings are based on the paper [1].
Fukushima Daiichi nuclear power plants (1F) were damaged by unprecedented severe accident in the Great East Japan Earthquake on 11 March 2011, and seawater has been injected as an emergency countermeasure for the core cooling. Although, the RPV and PCV were not supposed to be exposed to diluted seawater, they have been exposed to diluted seawater environment or high-moisture environment. Therefore, seawater corrosion has become an important issue. Immersion corrosion tests were performed for low-alloy steel of RPV material and carbon steel of PCV material in 1F cooling-water-simulated environment. As a result, the mass loss by corrosion was reduced with the decreasing temperature and chloride ion concentration. Moreover, the effects of nitrogen deaeration and Na 2 WO 4 addition on corrosion protection were remarkable among the selected corrosion countermeasures. In addition, the integrity assessments of RPV and PCV were performed considering the reduction of plate thickness based on corrosion test data and the load condition based on earthquake response analysis results. It had been confirmed that primary stresses for RPV and PCV equipment satisfied with the allowable values until at least 15 years after the accident.
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