Resveratrol ameliorates subacute intestinal ischemia-reperfusion injury

Dong, Wenfang; Li, FanFan; Pan, Zhiguo; Liu, Shenxi; Yu, Hao; Wang, Xianyue; Bi, Sheng; Zhang, WeiDa

doi:10.1016/j.jss.2013.05.013

Cited by 27 publications

(25 citation statements)

References 40 publications

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“…In this study, we chose 60 min of intestinal ischemia as described previously (28,29), a rather severe ischemic insult causing mortality from 52.9 to 100% (56,57), for our murine model. The lethal intestinal damage may minimize the difference in mortality; therefore, more animals may be needed in each group to detect the difference.…”

Section: Discussionmentioning

confidence: 99%

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Treatment with Recombinant Trichinella spiralis Cathepsin B–like Protein Ameliorates Intestinal Ischemia/Reperfusion Injury in Mice by Promoting a Switch from M1 to M2 Macrophages

Liu

Wen

Zhan

et al. 2015

The Journal of Immunology

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Intestinal ischemia/reperfusion (I/R) injury, in which macrophages play a key role, can cause high morbidity and mortality. The switch from classically (M1) to alternatively (M2) activated macrophages, which is dependent on the activation of STAT6 signaling, has been shown to protect organs from I/R injuries. In the current study, the effects of recombinant Trichinella spiralis cathepsin B–like protein (rTsCPB) on intestinal I/R injury and the potential mechanism related to macrophage phenotypes switch were investigated. In a mouse I/R model undergoing 60-min intestinal ischemia followed by 2-h or 7-d reperfusion, we demonstrated that intestinal I/R caused significant intestinal injury and induced a switch from M2 to M1 macrophages, evidenced by a decrease in levels of M2 markers (arginase-1 and found in inflammatory zone protein), an increase in levels of M1 markers (inducible NO synthase and CCR7), and a decrease in the ratio of M2/M1 macrophages. RTsCPB reversed intestinal I/R-induced M2–M1 transition and promoted M1-M2 phenotype switch evidenced by a significant decrease in M1 markers, an increase in M2 markers, and the ratio of M2/M1 macrophages. Meanwhile, rTsCPB significantly ameliorated intestinal injury and improved intestinal function and survival rate of animals, accompanied by a decrease in neutrophil infiltration and an increase in cell proliferation in the intestine. However, a selective STAT6 inhibitor, AS1517499, reversed the protective effects of rTsCPB by inhibiting M1 to M2 transition. These findings suggest that intestinal I/R injury causes a switch from M2 to M1 macrophages and that rTsCPB ameliorates intestinal injury by promoting STAT6-dependent M1 to M2 transition.

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Section: Discussionmentioning

confidence: 99%

“…Briefly, the small intestine was exteriorized with a 1-cm midline laparotomy, and the superior mesenteric artery (SMA) was identified. The SMA was occluded with a noncrushing microvascular clip for 60 min as described previously (28,29). Ischemia was recognized by the existence of pulseless or pale color of the small intestine.…”

Section: Animal Modelmentioning

confidence: 99%

Treatment with Recombinant Trichinella spiralis Cathepsin B–like Protein Ameliorates Intestinal Ischemia/Reperfusion Injury in Mice by Promoting a Switch from M1 to M2 Macrophages

Liu

Wen

Zhan

et al. 2015

The Journal of Immunology

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“…Resveratrol is a polyphenol which has significant protective effects against ischemia-reperfusion injury of various organs (9–14). Specifically, resveratrol has been shown to be protective in several animal models of renal I/R-induced injury through its anti-oxidant and anti-inflammatory effects (15–20).…”

Section: Introductionmentioning

confidence: 99%

Novel resveratrol analogues attenuate renal ischemic injury in rats

Khader

Yang

Kuncewitch

et al. 2015

Journal of Surgical Research

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Background Renal ischemia-reperfusion (I/R) is a severe clinical complication with no specific treatment. Resveratrol has been shown as a promising experimental agent in renal I/R due to its effect on cellular energy metabolism, oxidative stress, and inflammation. Recently, we identified two biologically active resveratrol analogues (RSVAs), RSVA405 and RSVA314. We hypothesized that both RSAVs would attenuate I/R-induced renal injury. Methods Adult male rats were subjected to renal I/R through bilateral renal pedicle clamping for 60 min, followed by reperfusion. RSVA405 (3 mg/kg BW), RSVA314 (3 mg/kg BW), or vehicle (10% DMSO and 33% Solutol in PBS) was administered by intraperitoneal injection 1 h prior to ischemia. Blood and renal tissues were collected 24 h after I/R for evaluation. Results Administration of RSVA405 and RSVA314 significantly reduced the serum levels of renal dysfunction and injury markers, including creatinine, blood urea nitrogen, aspartate aminotransferase, and lactate dehydrogenase, compared to vehicle. The protective effect of RSVA405 and RSVA314 was also reflected on histologic evaluation. Both RSVAs reduced the number of apoptotic cells by more than 60% as determined by TUNEL assay, compared to vehicle. The renal ATP levels of the vehicle group was decreased to 52.4% of control, while those of the RSVA405 and RSVA314 groups were restored to 72.3% and 79.6% of control, respectively. Both RSVAs significantly reduced the protein expression of inducible nitric oxide synthase and nitrotyrosine, and the mRNA levels of TNF-α, IL-6 and IL-1β. Conclusions RSVA405 and RSVA314 attenuate I/R-induced renal injury through the modulation of energy metabolism, oxidative stress, and inflammation.

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“…NO acts as a protective agent against I/R damage in organs (20,21). However, numerous studies have shown that following I/R, NO synthase may synthesize free oxygen radicals instead of NO (22,23).…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Resveratrol in a Rat Model of Ischemia-Reperfusion Injury of Sciatic Nerve

2017

GMJ

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Objective: The aim of this study was to determine the effects of resveratrol on ischemia-reperfusion (I/R) injury in the rats sciatic nerve. Methods: We used 18 Wistar rats anesthetized with ketamine (50 mg/kg-im). In the sham group, we only performed the surgery and not administer any medication. In the ischemia group, we generated I/R injury in 5 minute intervals with medical treatment of saline only. In the resveratrol group, we brought about the same I/R injury but gave resveratrol before the surgery by per oral. After sacrifice, we studied blood and nerve tissue samples. We evaluated the damage with malondialdehyde (MDA), nitric oxide (NO), and total antioxidant capacity (TAC), both in tissue and blood. Results: Evaluation of serum levels of antioxidant markers and tissue samples demonstrated statistically significant effects of resveratrol in ischemiareperfusion injury. Conclusion: This study revealed the antioxidant effect of resveratrol as an inhibitor of lipid peroxidation.

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Resveratrol ameliorates subacute intestinal ischemia-reperfusion injury

Cited by 27 publications

References 40 publications

Treatment with Recombinant Trichinella spiralis Cathepsin B–like Protein Ameliorates Intestinal Ischemia/Reperfusion Injury in Mice by Promoting a Switch from M1 to M2 Macrophages

Treatment with Recombinant Trichinella spiralis Cathepsin B–like Protein Ameliorates Intestinal Ischemia/Reperfusion Injury in Mice by Promoting a Switch from M1 to M2 Macrophages

Novel resveratrol analogues attenuate renal ischemic injury in rats

Protective Effects of Resveratrol in a Rat Model of Ischemia-Reperfusion Injury of Sciatic Nerve

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