Resveratrol and ascorbic acid prevent DNA damage induced by cryopreservation in human semen

Branco, Cátia Santos; Garcez, M.; Pasqualotto, Fábio Firmbach; Erdtman, Bernardo; Salvador, Mirian

doi:10.1016/j.cryobiol.2009.10.012

Cited by 118 publications

(77 citation statements)

References 6 publications

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“…For sperm cryopreservation, several factors, such as the pre-frozen sperm quality baseline, freezing and thawing method, and cryopreservative medium, and volume of cryopreserved specimen, have been previously demonstrated to be the crucial factors relating to post-thawing sperm outcome [2,7,21,23,24]. Several attempts have been made to improve cryopreservation techniques and media, leading to the improvement of cryopreservation [6,21,22,25]. Notably, the quality of ejaculated semen is also related to the outcome of cryopreservation.…”

Section: Introductionmentioning

confidence: 99%

“…For example, dead spermatozoa or leukocytes in pre-freezing semen detrimentally affect the sperm survival rate and the fertility potential after thawing through the ROS generation process [6]. A number of studies have demonstrated that supplemental addition of antioxidative to cryoprotective medium, e.g., ascorbate or catalase, minimize DNA damage and preserve sperm integrity; however, the benefit of these supplementations is still debated [25,26]. In this study, we hypothesized that sperm preparation before freezing, compared with sperm preparation after freezing-thawing, would improve post-thawed sperm, i.e., sperm motility, sperm vitality and non-apoptotic sperm.…”

Section: Introductionmentioning

confidence: 99%

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Sperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparation

Petyim

Neungton

Thanaboonyawat

et al. 2014

J Assist Reprod Genet

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Purpose To evaluate whether sperm preparation (swim-up technique) before freezing improves the percentages of sperm motility, sperm viability, and non-apoptotic spermatozoa after freezing-thawing process compared with preparation after cryopreservation. Methods Semen samples from 65 infertile males were equally divided into two aliquots one of which was processed for swim-up prior to cryopreservation and one of which was processed following cryopreservation. Sperm count, motility, and apoptosis index were measured in each group. Result (s) The total sperm count and the total motile sperm count decreased after thawing in both the pre-preparation and non-preparation groups compared with neat semen group (P<0.001). Moreover, the percentage of apoptotic sperm in the pre-preparation group after cryopreservation was lower than that in the non-preparation group (P<0.05), whereas the percentage of vital sperm with progressive motility was higher than that in the pre-preparation group (P<0.001). Conclusion (s) Semen preparation by swim-up before freezing resulted in better sperm quality and fewer apoptotic sperm than sperm preparation after thawing. Therefore, sperm preparation before cryopreservation should be considered in routine sperm cryopreservation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparation

Petyim

Neungton

Thanaboonyawat

et al. 2014

J Assist Reprod Genet

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“…107,108 Several studies have also examined the role of antioxidants in protecting sperm DNA from injury following cryopreservation and thawing. Most studies have shown that antioxidants (vitamin C, catalase, resveratrol and genistein) can protect the sperm DNA from oxidative injury during cryopreservation and subsequent thawing [109][110][111][112] ( Table 6). In contrast, Taylor et al reported that the antioxidant vitamin E does not protect sperm DNA during cryopreservation.…”

Section: Role Of In Vitro Antioxidants In Protecting Spermatozoa Frommentioning

confidence: 99%

Antioxidant therapy in male infertility: fact or fiction?

Zini¹,

Alhathal²

2011

Asian J Androl

110

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Infertile men have higher levels of semen reactive oxygen species (ROS) than do fertile men. High levels of semen ROS can cause sperm dysfunction, sperm DNA damage and reduced male reproductive potential. This observation has led clinicians to treat infertile men with antioxidant supplements. The purpose of this article is to discuss the rationale for antioxidant therapy in infertile men and to evaluate the data on the efficacy of dietary and in vitro antioxidant preparations on sperm function and DNA damage. To date, most clinical studies suggest that dietary antioxidant supplements are beneficial in terms of improving sperm function and DNA integrity. However, the exact mechanism of action of dietary antioxidants and the optimal dietary supplement have not been established. Moreover, most of the clinical studies are small and few have evaluated pregnancy rates. A beneficial effect of in vitro antioxidant supplements in protecting spermatozoa from exogenous oxidants has been demonstrated in most studies; however, the effect of these antioxidants in protecting sperm from endogenous ROS, gentle sperm processing and cryopreservation has not been established conclusively. Keywords: antioxidant; male infertility; oxidative stress; sperm DNA fragmentation; vitamins RELATIONSHIP BETWEEN OXIDATIVE STRESS AND SPERM DYSFUNCTION Seminal oxidative stress (OS) results from an imbalance between reactive oxygen species (ROS) production and ROS scavenging by seminal antioxidants. Seminal OS is believed to be one of the main factors in the pathogenesis of sperm dysfunction and sperm DNA damage in male infertility.1-4 Indeed, it is estimated that 25% of infertile men possess high levels of semen ROS, whereas fertile men do not have high levels of semen ROS.1,4-6 Although a controlled production of these ROS is required for sperm physiology (sperm hyperactivation, capacitation and acrosome reaction) and for natural fertilization, [7][8][9] the excessive production of ROS by immature germ cells and leukocytes causes sperm dysfunction (lipid peroxidation, loss of motility and sperm DNA damage). 9,10 Spermatozoa are particularly susceptible to oxidative injury due to the abundance of plasma membrane polyunsaturated fatty acids. [10][11][12] These unsaturated fatty acids provide fluidity that is necessary for membrane fusion events (e.g., the acrosome reaction and sperm-egg interaction) and for sperm motility. However, the unsaturated nature of these molecules predisposes them to free radical attack and ongoing lipid peroxidation throughout the sperm plasma membrane. Once this process has been initiated, accumulation of lipid peroxides occurs on the sperm surface (this results in loss of sperm motility) and oxidative damage to DNA can ensue. 13,14SEMINAL ANTIOXIDANT CAPACITY AND SPERM DYSFUNCTION Seminal plasma and spermatozoa themselves are well endowed with an array of protective antioxidants to protect spermatozoa from OS, especially, at the post-testicular level. 6,15,16 Seminal plasma contains a number of high-molecular weigh...

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“…Therefore, protecting the spermatozoa from cryopreservation-induced DNA damage and loss of sperm functional competence is clinically relevant in assisted reproductive technology (ART). Taking this into consideration, the most common approach followed hitherto, was to protect the sperm DNA during freeze-thaw process by using free radical scavengers along with the cryopreservation medium [12,13].…”

Section: Introductionmentioning

confidence: 99%

Addition of zinc to human ejaculate prior to cryopreservation prevents freeze-thaw-induced DNA damage and preserves sperm function

Kotdawala

Kumar

Salian

et al. 2012

J Assist Reprod Genet

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Purpose To study the effect of addition of zinc to human semen sample prior to cryopreservation on post-thaw sperm quality and function. Methods Semen samples were collected from men attending university infertility clinic for semen analysis (n0109). Liquefied semen samples were cryopreserved in glycerol-egg yolkcitrate medium with or without the prior addition of zinc (100 μM) and stored in liquid nitrogen. After 10 days, the semen samples were thawed to assess the outcome. Sperm motility, DNA integrity, mitochondrial potential and the ability of spermatozoa to undergo capacitation and acrosome reaction was assessed in post-thaw samples. Results Semen samples cryopreserved after addition of zinc had a significantly higher percentage of sperm with intact DNA (p<0.001), mitochondrial function (p<0.001) and progressive motility (p<0.01) compared to the semen samples cryopreserved without zinc supplementation. Apart from this, ability to undergo capacitation and acrosome reaction in vitro was significantly higher in semen samples cryopreserved with zinc (p<0.0001 and p<0.001 respectively).Conclusions Addition of zinc to semen samples prior to cryopreservation helps in preventing the freeze-thaw-induced sperm DNA damage and loss of sperm function.

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Resveratrol and ascorbic acid prevent DNA damage induced by cryopreservation in human semen

Cited by 118 publications

References 6 publications

Sperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparation

Sperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparation

Antioxidant therapy in male infertility: fact or fiction?

Addition of zinc to human ejaculate prior to cryopreservation prevents freeze-thaw-induced DNA damage and preserves sperm function

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