Resveratrol attenuates oxidative stress and histological alterations induced by liver ischemia/reperfusion in rats

Abstract

AIM:To investigate the effects of resveratrol on liver ischemia/reperfusion (I/R) injury in rats. METHODS: A total of 40 male Sprague-Dawley rats weighing 240-290 g were randomized into four groups of ten: (1) controls: data from unmanipulated animals; (2) sham group: rats subjected to the surgical procedure, except for liver I/R, and given saline; (3) I/R group: rats underwent liver ischemia for 45 min followed by reperfusion for 45 min; (4) I-R/Resveratrol group: rats pretreated with resveratrol (10 µmol/L, iv). Liver tissues were obtained to determine antioxidant enzyme levels and for biochemical and histological evaluation. RESULTS: Plasma aminotransferase activities were higher in the I/R group than in the I-R/Resveratrol group. Malondialdehyde levels and the hepatic injury score decreased, while superoxide dismutase, catalase, and glutathione peroxidase levels increased in group 4 compared to group 3. In group 4, histopathological changes were significantly attenuated in resveratroltreated livers. CONCLUSION: These results suggest that resveratrol has protective effects against hepatic I/R injury, and is a potential therapeutic drug for ischemia reperfusionrelated liver injury.

Resveratrol Reduces Morphologic Changes in the Myenteric Plexus and Oxidative Stress in the Ileum in Rats with Ischemia/Reperfusion Injury

“…Only laparotomy was performed on the sham groups. The treated groups received resveratrol at a concentration of 10 mg/kg/day as previously reported [22,23]. Resveratrol was administered via gavage once per day for 5 days before surgery, and it was dissolved in a vehicle solution of 80 % water and 20 % ethanol as previously reported [24].…”

Hypothermic Machine Preservation Reduces Molecular Markers of Ischemia/Reperfusion Injury in Human Liver Transplantation

“…However, because the circulating hypothermic solution is capable of carrying far more oxygen than its warm counterpart, it is unclear whether HMP needs to be actively oxygenated (17)(18)(19) or whether simple oxygen diffusion (3) is sufficient to maintain viable oxygen levels. Oxidative damage due to reactive oxygen species (ROS) is a pivotal injury in determining the severity of PI (20)(21)(22)(23)(24). The increase seen in super-oxide dismutase (SOD) postreperfusion in the SCS group ( Figure 1C) indicates the cell's attempt to balance the presence of high levels of oxygen radicals produced due to the oxidative stress incurred by hypothermia and hypoxia.…”

Ischemic post-conditioning attenuates the intestinal injury induced by limb ischemia/reperfusion in rats

“…The production of reactive oxygen metabolites during ischemia-reperfusion involves oxidases found in resident phagocytic cells as well as microvascular and mucosal epithelial cells (25). In the present study, the small intestinal tissue SOD activity of group LIR during reperfusion was lower than that of group S, but the small intestinal tissue MPO activity and MDA concentrations were higher.…”