Resveratrol impairs cellular mechanisms associated with the pathogenesis of endometriosis

Madanes, Daniela; Meresman, Gabriela Fabiana; Valla, S; Hassan, Nourhan; Kiesel, L.; Greve, Burkhard; Barañao, Rosa Inés; Götte, Martin; Ricci, Analía Gabriela

doi:10.1016/j.rbmo.2022.02.008

Cited by 14 publications

(16 citation statements)

References 86 publications

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“…The wound healing assay was carried out using an established procedure 15 . T‐HESC cells (100,000 cells/well) were plated in 24 well plates and maintained in complete medium for 24 h or until 100% confluence was achieved.…”

Section: Methodsmentioning

confidence: 99%

“…The wound healing assay was carried out using an established procedure. 15 T-HESC cells (100,000 cells/ well) were plated in 24 well plates and maintained in complete medium for 24 h or until 100% confluence was achieved. Then, cells were washed thoroughly with PBS, a scratch was done with a 100 μl pipette tip and the stimuli were added in 0% FBS containing medium.…”

Section: Wound Healing (Scratch) Assaymentioning

confidence: 99%

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Effects of pharmacological inhibition of hyaluronic acid synthesis on experimental endometriosis

Olivares

Ricci

Bilotas

et al. 2022

Eur J Clin Investigation

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Background Dysregulated hyaluronic acid (HA) metabolism has been shown to be implicated in several pathologies including endometriosis. 4‐Methylumbelliferone (4MU) is an HA synthesis inhibitor with proven antitumour activity. In this study, we aim to evaluate the effect of 4MU on endometriosis development both in vivo and in vitro. Methods Endometriosis was surgically induced by uterine tissue auto‐transplantation in 32 two‐month‐old BALB/c mice. Animals were designated into the early or late starting treatment group, which initiated on day 2 or day 15 after surgery, respectively. Within each group, 4MU 200 mg/kg/day or vehicle (Control) were administered by oesophageal gavage for 28 days. After sacrifice, the percentage of developed lesions, lesion size, cell proliferation, vascularization and HA deposition within the endometriotic‐like lesions were evaluated. Cell viability was assessed in endometrial epithelial cells (ECC‐1) and in endometrial stromal cells (t‐HESC); and migration was evaluated in t‐HESC. Results There was a significant reduction in the percentage of developed lesions in mice that started the 4MU treatment on day 2 compared with its respective control group, and compared with those that started treatment on day 15. However, no significant changes were found when analysing endometriotic‐like lesion's cell proliferation, vascularization and HA deposition. In vitro, both cell viability and migration were inhibited by 4MU treatment. Conclusions The inhibition of HA synthesis could be a beneficial and alternative option to treat endometriosis at the early stage of the disease. Further research is necessary to elucidate 4MU's mechanism of action and better strategies for delivering this promising drug.

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Section: Methodsmentioning

confidence: 99%

Section: Wound Healing (Scratch) Assaymentioning

confidence: 99%

Effects of pharmacological inhibition of hyaluronic acid synthesis on experimental endometriosis

Olivares

Ricci

Bilotas

et al. 2022

Eur J Clin Investigation

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“…Madanes et al investigated the mechanism of resveratrol in endometrial cells, and they reported that this compound remarkably reduces cell viability and induces apoptosis by increasing caspase-3 cleavage in two cell lines, namely, endometriotic epithelial cells (12Z) and endometrial stromal cells (St-T1b). 55 The property of invasiveness depends on the ratio of matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs). 56 Furthermore, resveratrol mitigates the ability of invasion and angiogenesis by lowering the gene expression ratio of MMP-2/TIMP-1 and suppressing the mRNA expression of vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1).…”

Section: Molecular Mechanisms Of Resveratrol Against Endometriosismentioning

confidence: 99%

“… 56 Furthermore, resveratrol mitigates the ability of invasion and angiogenesis by lowering the gene expression ratio of MMP-2/TIMP-1 and suppressing the mRNA expression of vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1). 55 …”

Section: Molecular Mechanisms Of Resveratrol Against Endometriosismentioning

confidence: 99%

Review of the Potential Therapeutic Effects and Molecular Mechanisms of Resveratrol on Endometriosis

Jiang¹,

Chen²,

Gu³

et al. 2023

IJWH

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Endometriosis is a hormone-dependent inflammatory disease characterized by the existence of endometrial tissues outside the uterine cavity. Pharmacotherapy and surgery are the current dominant management options for endometriosis. The greater incidence of recurrence and reoperation after surgical treatment as well as the adverse effects of medical approaches predispose patients to potential limitations for their long-term usage. Consequently, it is essential to explore novel supplementary and alternative drugs to ameliorate the therapeutic outcomes of endometriotic patients. Resveratrol is a phenolic compound that has attracted increasing interest from many researchers due to its pleiotropic biological activities. Here, we review the possible therapeutic efficacies and molecular mechanisms of resveratrol against endometriosis based on in vitro, animal, and clinical studies. The potential mechanisms of resveratrol include anti-proliferative, pro-apoptotic, anti-angiogenic, anti-oxidative stress, anti-invasive and anti-adhesive effects, thereby suggesting that resveratrol is a promising candidate for endometriosis. Because most studies have investigated the effectiveness of resveratrol on endometriosis via in vitro trials and/or experimental animal models, further high-quality clinical trials should be undertaken to comprehensively estimate the clinical application feasibility of resveratrol on endometriosis.

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“…Plant extracts, including pueraria lobata and black garlic extracts, have been widely confirmed to play an anti-inflammatory, antiangiogenic and antioxidant roles in endometrial cells. Bioactive substances, including resveratrol, catechin, and curcumin, are believed to modulate a variety of signaling pathways and have therapeutic effects in endometriosis ( Kamal et al., 2021 ; Hipolito-Reis et al., 2022 ; Madanes et al., 2022 ). Although botanical drugs have shown good “potential” in vitro and in animal experiments, the data from clinical double-blind randomized controlled trials are still insufficient.…”

Section: Role Of the Gut Microbiota In The Pathogenesis Of Endometriosismentioning

confidence: 99%

The gut microbiota and endometriosis: From pathogenesis to diagnosis and treatment

Qin

Tian²,

Liu³

et al. 2022

Front. Cell. Infect. Microbiol.

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Endometriosis is a common gynecological disease, that often leads to pain and infertility. At present, the specific pathogenesis of endometriosis has not been clarified, but it may be closely related to an imbalance of sex hormones in the body, ectopic hyperplasia stimulated by immune inflammation, and invasion and escape based on tumor characteristics. Gut microbiota is associated with many inflammatory diseases. With the further study of the gut microbiota, people are paying increasing attention to its relationship with endometriosis. Studies have shown that there is an association between the gut microbiota and endometriosis. The specific ways and mechanisms by which the gut microbiota participates in endometriosis may involve estrogen, immune inflammation, and tumor characteristics, among others. Therefore, in the future, regulating gut microbiota disorders in various ways can help in the treatment of endometriosis patients. This study reviewed the research on the gut microbiota and endometriosis in order to provide ideas for clinical diagnosis and treatment.

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Resveratrol impairs cellular mechanisms associated with the pathogenesis of endometriosis

Cited by 14 publications

References 86 publications

Effects of pharmacological inhibition of hyaluronic acid synthesis on experimental endometriosis

Effects of pharmacological inhibition of hyaluronic acid synthesis on experimental endometriosis

Review of the Potential Therapeutic Effects and Molecular Mechanisms of Resveratrol on Endometriosis

The gut microbiota and endometriosis: From pathogenesis to diagnosis and treatment

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