Resveratrol inhibits the protein expression of transcription factors related adipocyte differentiation and the activity of matrix metalloproteinase in mouse fibroblast 3T3-L1 preadipocytes

Abstract

This study attempted to investigate the effects of resveratrol on the differentiation of adipocytes. After cells were treated with various concentrations of resveratrol (0, 10, 20, and 40 µmol/L), adipocyte proliferation, the protein expression of transcription factors, and MMPs' activities were determined. Cell proliferation was inhibited more within 4 days of incubation (P < 0.05), and lipid accumulation in adipocyte was significantly inhibited by 93.8%, 92.4% and 91.5%, respectively, after two days of 10, 20, and 40 µmol/L resveratrol treatment (P < 0.05). Six days of incubation with the three resveratrol concentrations caused a significantly decreases of 63%, 59.9%, and 25.1% GPDH activity as a dose-dependent response. The triglyceride concentration also decreased significantly with the increase of resveratrol concentration (P < 0.05). The protein expression of CCAAT/enhancer-binding protein (C/EBPβ) was decreased significantly by 56% and 30% while PPARγ was significantly reduced by 57% and 15% with resveratrol treatments of 20 and 40 µmol/L, respectively (P < 0.05). The protein expression of C/EBPα was decreased by 83%, 74%, and 38% to increased dosage levels, with significance determined for this decrease from 20 µmol/L of resveratrol. The protein expression of fatty acid binding protein (FABP4) was decreased significantly by 88%, 72%, and 46% with the increase of resveratrol concentration. The activity of MMP-2 was decreased significantly by 84%, 70%, and 63% while MMP-9 activity was decreased significantly by 74%, 62%, and 39% with the increased resveratrol concentrations of 10, 20, and 40 µmol/L, respectively (P < 0.05).

Protective effect of resveratrol on arsenic trioxide-induced nephrotoxicity in rats

“…Maiti and Chatterjee [20] reported that GSH also stimulated the arsenic detoxification processes by modulating arsenic methylation and increasing hepatobiliary excretion of arsenic. Resveratrol is a naturally occurring stilbene, a potent free radical scavenger and may also stimulate endogenous antioxidant enzyme activities [21,22]. As expected, As2O3 treatment resulted in significant nephrotoxicity in rats, and pre-treatment with resveratrol attenuated or prevented the oxidative stress, thereby mitigating the subsequent renal damage.…”

Does Resveratrol Have a Protective Effect on Tympanosclerosis Formation in Experimentally Induced Otitis Media?

“…Several studies have shown that resveratrol may be involved in the regulation of MMP-2 and MMP-9 activity [22,23] . Gao et al [22] showed that resveratrol reduced the elevated level of MMP-9 induced by cerebral ischaemia-reperfusion in mice, and Kang et al [23] demonstrated that the activity of MMP-2 and MMP-9 decreased significantly at different resveratrol concentrations, with MMP-9 being more significantly affected than MMP-2.…”

“…Guo et al [21] reported changes in expression of TGF-β1 and MMP-9 in the formation of TS. In addition, several studies have reported that resveratrol affects MMP activity in adipose and neuronal tissues [22,23] .…”

Use of Polyphenolic Compounds in Dermatologic Oncology

“…Finally, resveratrol has been shown to be a pro-apoptotic compound against melanoma [219], inducing apoptosis once it induces G1/S cell cycle arrest [27, 220–223], through expression of dihydronicotinamide riboside quinone reductase 2 and p53 [221], upregulation of cyclins A, E, and B1 [223], suppression of STAT-3/β-catenin-pathway [224], and production of NO, which stimulates p53 and Bax-Bcl2 mitochondrial apoptosis [225, 226]. Subsequently, cytochrome C, and caspase-9 and -3 are released [227, 228].…”