Resveratrol prevents nicotine-induced teratogenesis in cultured mouse embryos

Cai, Lin; Yon, Jung-Min; Jung, A Young; Lee, Jong Geol; Jung, Ki Youn; Kang, Jong-Koo; Lee, Beom Jun; Yun, Young Won; Nam, Sang-Yoon

doi:10.1016/j.reprotox.2012.05.097

Cited by 22 publications

(28 citation statements)

References 42 publications

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“…Nicotine induces oxidative stress both in vivo and in vitro [9]. Recently, we found that resveratrol, a natural polyphenol compound, prevents nicotine-induced teratogenesis in cultured mouse embryos through its potent antioxidative activity [5]. In the current study, nicotine increased the MDA level and decreased the SOD activity in embryos.…”

Section: Discussionsupporting

confidence: 51%

“…Although effective smoking cessation strategies during pregnancy are important for maternal and fetal health, previous studies have suggested that an alternative therapy may be to use natural antioxidant treatments that can protect against nicotine-induced embryo toxicity [5, 18]. In the present study, we expanded upon this concept not only to demonstrate the beneficial effects of β -carotene against nicotine-induced damage, but also to distinguish the mechanisms of nicotine damage further using an embryo culture system.…”

Section: Discussionmentioning

confidence: 94%

“…After removing the decidua and Reichert's membranes, embryos with intact visceral yolk sacs and ectoplacental cones were placed randomly into sealed culture bottles (three embryos/bottle) containing 3 mL of culture medium and different concentrations (1 × 10 −7 or 5 × 10 −7 μ M) of β -carotene (Sigma, St. Louis, MO, USA) dissolved in dimethyl sulfoxide (DMSO, Sigma) and/or 1 mM nicotine (163.8 μ g/mL serum; Sigma). The nicotine concentration used here was determined by previous studies [5, 11]. The final concentration of DMSO in the medium was less than 0.1%.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, we found that resveratrol, a polyphenol from red wine, can prevent nicotine-induced teratogenesis in cultured mouse fetuses [5]. Dietary micronutrients such as vitamin C, vitamin E, and β -carotene contribute to the antioxidant defense system [6].…”

Section: Introductionmentioning

confidence: 99%

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Antiteratogenic Effects ofβ-Carotene in Cultured Mouse Embryos Exposed to Nicotine

Cai

Yon

Jung

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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After maternal intake, nicotine crosses the placental barrier and causes severe embryonic disorders and fetal death. In this study, we investigated whether β-carotene has a beneficial effect against nicotine-induced teratogenesis in mouse embryos (embryonic day 8.5) cultured for 48 h in a whole embryo culture system. Embryos exposed to nicotine (1 mM) exhibited severe morphological anomalies and apoptotic cell death, as well as increased levels of TNF-α, IL-1β, and caspase 3 mRNAs, and lipid peroxidation. The levels of cytoplasmic superoxide dismutase (SOD), mitochondrial manganese-dependent SOD, cytosolic glutathione peroxidase (GPx), phospholipid hydroperoxide GPx, hypoxia inducible factor 1α, and Bcl-x L mRNAs decreased, and SOD activity was reduced compared to the control group. However, when β-carotene (1 × 10−7 or 5 × 10−7 μM) was present in cultures of embryos exposed to nicotine, these parameters improved significantly. These findings indicate that β-carotene effectively protects against nicotine-induced teratogenesis in mouse embryos through its antioxidative, antiapoptotic, and anti-inflammatory activities.

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antiteratogenic Effects ofβ-Carotene in Cultured Mouse Embryos Exposed to Nicotine

Cai

Yon

Jung

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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“…As a polyphenol, resveratrol can scavenge ROS with its phenolic hydroxyl group in oocytes. Besides, resveratrol could also protect embryo from nicotine induced oxidative stress [42]. In this case, resveratrol protected oocytes from mancozeb increased ROS, with the evidence of decreased fluorescence intensity of DCFH-DA, suggesting an improved condition for oocyte development formed, indirectly preventing subsequent mancozeb induced apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of resveratrol against mancozeb induced apoptosis damage in mouse oocytes

Liu

Wang

et al. 2016

Oncotarget

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Mancozeb, a mixture of ethylene-bis-dithiocarbamate manganese and zinc salts, is one of the most widely used fungicides in agriculture. Mancozeb could lead to mitochondria dysfunction, cellular anti-oxidation enzymes depletion and apoptotic pathways activation. Previous studies indicated the exposure of mancozeb through mother would lead to irregular estrous cycles, decreased progesterone levels, reduced litter sizes, and more frequent delivery of dead fetuses. In this study, we investigated mancozeb inducing reproductive toxicity, especially focusing on its apoptotic effect and epigenetic modifications. We also showed that resveratrol, a kind of phytoalexin found in peanuts and grapes, can alleviate mancozeb's adverse effects, such as declined fertility, decreased ovary weight and primary follicles. Besides, mancozeb treated oocytes displayed suboptimal developmental competence and this can also be improved by treatment of resveratrol. More detailed investigation of these processes revealed that mancozeb increased reactive oxygen species, causing cell apoptosis and abnormal epigenetic modifications, and resveratrol can block these cytotoxic changes. Collectively, our results showed that resveratrol can alleviate mancozeb induced infertility and this was mainly through the correction of apoptotic tendency and the abnormity of cellular epigenetic modification.

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Developmental toxicity of e‐cigarette aerosols

Greene

Pisano

2019

Birth Defects Research

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Maternal smoking during pregnancy represents a major public health concern increasing the risk for low birth weight, congenital anomalies, preterm birth, fetal mortality, and morbidity. In an effort to diminish adverse developmental effects of exposure to cigarette smoking, pregnant women, and women of reproductive age, are increasingly turning to electronic nicotine delivery systems (ENDS), such as e‐cigarettes, as an alternative. Given that health risks associated with ENDS use during pregnancy are largely unknown, there is an acute need to determine risks vs. benefits of e‐cigarette use by pregnant women. While the most recent Surgeon General's Report on the “Health Consequences of Smoking” states that “the evidence is sufficient to infer that nicotine adversely affects maternal and fetal health during pregnancy, contributing to multiple adverse outcomes,” it remains unclear whether use of ENDS represents a “safer alternative” to tobacco smoking during pregnancy. This is due, in part, to the lack of sufficient and conclusive evidence concerning whether or not maternal e‐cigarette use adversely affects embryonic/fetal development. While several recent developmental studies have challenged the safety of nicotine inhalation via ENDS, the true risks of smoking e‐cigarettes during the first trimester of pregnancy—the period of organogenesis—are largely unknown. Moreover, evidence is emerging that even nicotine‐free e‐cigarette aerosols may harm the developing conceptus, suggesting that components of e‐cigarette liquid, including flavorings, may be developmentally toxicity. Focused human epidemiological analyses, and carefully designed animal studies are critically needed to address the question of the safety of ENDS use during pregnancy.

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Resveratrol prevents nicotine-induced teratogenesis in cultured mouse embryos

Cited by 22 publications

References 42 publications

Antiteratogenic Effects ofβ-Carotene in Cultured Mouse Embryos Exposed to Nicotine

Antiteratogenic Effects ofβ-Carotene in Cultured Mouse Embryos Exposed to Nicotine

Protective effects of resveratrol against mancozeb induced apoptosis damage in mouse oocytes

Developmental toxicity of e‐cigarette aerosols

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