Resveratrol Protects against Helicobacter pylori-Associated Gastritis by Combating Oxidative Stress

Abstract

Helicobacter pylori (H. pylori)-induced oxidative stress has been shown to play a very important role in the inflammation of the gastric mucosa and increases the risk of developing gastric cancer. Resveratrol has many biological functions and activities, including antioxidant and anti-inflammatory effect. The purpose of this study was to probe whether resveratrol inhibits H. pylori-induced gastric inflammation and to elucidate the underlying mechanisms of any effect in mice. A mouse model of H. pylori infection was established via oral inoculation with H. pylori. After one week, mice were administered resveratrol (100 mg/kg body weight/day) orally for six weeks. The mRNA and protein levels of iNOS and IL-8 were assessed using RT-PCR, Western blot and ELISA. The expression levels of IκBα and phosphorylated IκBα (which embodies the level and activation of NF-κB), Heme Oxygenase-1 (HO-1; a potent antioxidant enzyme) and nuclear factor-erythroid 2 related factor 2 (Nrf2) were determined using Western blot, and lipid peroxide (LPO) level and myeloperoxidase (MPO) activity were examined using an MPO colorimetric activity assay, thiobarbituric acid reaction, and histological-grade using HE staining of the gastric mucosa. The results showed that resveratrol improved the histological infiltration score and decreased LPO level and MPO activity in the gastric mucosa. Resveratrol down-regulated the H. pylori-induced mRNA transcription and protein expression levels of IL-8 and iNOS, suppressed H. pylori-induced phosphorylation of IκBα, and increased the levels of HO-1 and Nrf2. In conclusion, resveratrol treatment exerted significant effects against oxidative stress and inflammation in H. pylori-infected mucosa through the suppression of IL-8, iNOS, and NF-κB, and moreover through the activation of the Nrf2/HO-1 pathway.

Production of Resveratrol by Piceid Deglycosylation Using Cellulase

“…It has been suggested that moderate drinking of red wine helps to prevent cardiovascular disease; resveratrol in red wine is believed to be the key molecule due to its inhibitory activities against platelet aggregation and oxidation of low-density lipoprotein (LDL) in blood [4]. Resveratrol is also recognized as a bioactive agent with potent anti-inflammatory, antioxidant, anti-proliferative, and cancer chemopreventive activities [5,6].…”

Phytochemicals enhance antioxidant enzyme expression to protect against NSAID‐induced oxidative damage of the gastrointestinal mucosa

“…Zhang et al. also indicated that mice treated with resveratrol (100 mg/kg b.w. for 6 weeks) could against H. pylori (10 8 CFU)‐induced IL‐8, iNOS, and NF‐κB expressions through activation of the Nrf2/HO‐1 pathway in gastric tissue.…”

“…Some reports indicated that dietary phenolic compounds (curcumin, mangiferin, and β‐myrcene), phenolic acids (caffeic acid and ellagic acid), flavan‐3‐ols (catechin and (−)‐epigallocatechin‐3‐gallate [EGCG]), flavonols (quercetin and morin), flavonoids (resveratrol and pogostone), gingerols (6‐gingerol and 6‐shogaol), carotenes (lycopene and β‐carotene), and organosulfur ( s ‐allyl cysteine) possess multiple bioactivity and potential health benefits, such as antioxidant, anti‐inflammatory, and antibacterial benefits, and they can prevent digestive disease processes . In addition, some of the most beneficial phytochemicals also appear to increase antioxidant enzymes, mucosal defense, and the capacity to heal ulcers via upregulation of the Nrf2 signaling pathway, which may aid mucosal repair . These results suggest that Nrf2 expression induced by phytochemicals/pharmacological modulators may be a novel means of increasing GI protection.…”

A Study on Peanut Spouts Extract as the Anti-oxidant Activity and the Skin Whitening Cosmetic Ingredients

“…4). 이러한 결과는 땅콩새싹의 주성분으로 알려진 resveratrol이 폴리페놀계 파이토알렉신 (phytoalexins)의 작용 으로 항염증 작용이 우수한 것으로 알려져 있고 [19], 특히 포 도, 땅콩의 열매에서 발견되는 resveratrol은 대식세포, 지방 세포와 기질 혈관 세포에서 항염증 효과를 나타내고 있는 기 존 연구와 유사하다 [20]. 따라서 땅콩새싹 추출물에 포함되 어 있는 다수의 성분 중 resveratrol의 작용에 의해 NO의 생성 을 억제한 것으로 사료되며 NO에 의한 염증반응으로 나타나 는 피부 노화 및 주름생성을 지연시킬 수 있는 화장품 소재로 써의 가능성이 있는 것으로 사료된다.…”