Resveratrol reduces lipid peroxidation and increases sirtuin 1 expression in adult animals programed by neonatal protein restriction

Abstract

Resveratrol (Res) has been associated with protective effects against oxidative stress. This study evaluated the effect of Res over lipid peroxidation, antioxidant defense, hepatic sirtuin 1 (SIRT1), which up-regulates antioxidant enzymes, and copper/zinc superoxide dismutase (Cu/Zn SOD) in adult offspring whose mothers were protein restricted during lactation. Lactating Wistar rats were divided into control (C) group, which were fed a normal diet (23% protein), and low-protein and high-carbohydrate (LPHC) group, which were fed a diet containing 8% protein. After weaning (21 days), C and LPHC offspring were fed a normal diet until they were 180 days old. At the 160th day, animals were separated into four groups as follows: control, controlCRes, LPHC, and LPHCCRes. Resveratrol was given for 20 days (30 mg/kg per day by gavage). LPHC animals showed a higher total antioxidant capacity (TAC) without change in lipid peroxidation and SIRT1 expression. The treatment with Res increased TAC only in the control group without effect on lipid peroxidation and SIRT1. LPHC animals treated with Res had lower lipid peroxidation and higher protein and mRNA expression of SIRT1 without any further increase in TAC. No significant difference in liver Cu/Zn SOD expression was observed among the groups. In conclusion, maternal protein restriction during lactation programs the offspring for a higher antioxidant capacity, and these animals seem to respond to Res treatment with a lower lipid peroxidation and higher hepatic SIRT1 expression that we did not observe in the Res-treated controls. It is probable that the protective effect can be attributed to Res activating SIRT1, only in the LPHC-programed group.

Resveratrol: A Candidate Drug for Treating Rheumatoid Arthritis

“…Notably, resveratrol is shown to increase expression of sirtuin 1, a mammalian NAD + -dependent deacetylase (class III HDAC) (Franco et al, 2010). In the present study, resveratrol upregulated expression of mRNAs for FOXO-1, FOXO-3, p21, p27, AIF, and sirtuin 1 in MH7A cells but otherwise downregulated expression of the Bcl-X L mRNA, without affecting expression of mRNAs for p53, Bcl-2, Bad, Bax, or Bak.…”

Resveratrol treatment rescues hyperleptinemia and improves hypothalamic leptin signaling programmed by maternal high-fat diet in rats

“…Leptin gene expression was quantified as described before [4,10]. Total RNA was isolated from adipose tissue using commercial kit (RNeasy lipid tissue mini kit, Qiagen, Texas, USA).…”

“…In the purpose to investigate new therapeutic possibilities for weight control in programming models of obesity, our group has been focusing on the effects of resveratrol as a therapeutic supplement to treat metabolic diseases [10,11].…”

Calcium supplementation reverts central adiposity, leptin, and insulin resistance in adult offspring programed by neonatal nicotine exposure

“…Furthermore, increased SIRT1 has been associated with lower adiposity and protection against dietinduced metabolic disorders (Banks et al 2008, Pfluger et al 2008. Therefore, we reasoned that SIRT1 expression may be altered by programming and/or calcium treatment, as we have previously reported for other programming models (Franco et al 2010, Trotta et al 2011. (Marques et al 2009).…”