Resveratrol reduces the expression of insulin‐like growth factor‐1 and hepatocyte growth factor in stromal cells of women with endometriosis compared with nonendometriotic women

Arablou, Tahereh; Delbandi, Ali‐Akbar; Khodaverdi, Sepideh; Arefi, Soheila; Kolahdouz‐Mohammadi, Roya; Heidari, Samaneh; Mohammadi, Tahereh; Aryaeian, Naheed

doi:10.1002/ptr.6298

Cited by 33 publications

(54 citation statements)

References 53 publications

(66 reference statements)

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“…Increased HGF expression has been shown in ectopic compared to eutopic endometrial tissue in patient with endometriosis [43] or non-endometriotic patients [53]. Regarding ESCs, one study showed increased HGF protein expression in EuESCs compared to CESCs [54] and in line with our findings, in a study by Arablou et al HGF gene expression was higher in EESCs compared to EuESCs [55].…”

Section: Discussionsupporting

confidence: 90%

Evaluation of apoptosis and angiogenesis in ectopic and eutopic stromal cells of patients with endometriosis compared to non-endometriotic controls

et al. 2020

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Background: Endometriosis is a chronic, painful, and inflammatory disease characterized by extra-uterine growth of endometrial tissues. Increased angiogenesis and resistance to apoptosis have been suggested to be involved in pathogenesis and development of endometriosis. The objective of this study was to examine apoptosis potential and angiogenesis contribution of eutopic (EuESCs) and ectopic (EESCs) endometrial stromal cells in patients with endometriosis compared to endometrial stromal cells from non-endometriotic controls (CESCs). Methods: Stromal cells were isolated by enzymatic digestion of ectopic (n = 11) and eutopic (n = 17) endometrial tissues from laparoscopically-confirmed endometriotic patients. Endometrial stromal cells of 15 non-endometriotic patients served as control. Following cell characterization by immunofluorescent staining and flow cytometry using a panel of antibodies, the total RNA was isolated from the cultured cells, and analyzed for the expression of genes involved in apoptosis (Bcl-2, Bcl-xL, Bax, and caspase-3) and angiogenesis [vascular endothelial growth factor-A (VEGF-A) and hepatocyte growth factor (HGF)] by Real-time PCR. Results: Significantly higher gene expression levels of Bcl-2 and Bcl-xL were found in EESCs compared with EuESCs and CESCs (p < 0.01). The gene expression of Bax in EESCs, EuESCs, and CESCs was not statistically significant. Furthermore, EuESCs exhibited a significantly lower caspase-3 gene expression compared with CESCs (p < 0.01) or EESCs (p < 0.05). Regarding angiogenesis, VEGF-A gene expression in EESCs (p < 0.001) and EuESCs (p < 0.05) were significantly higher compared with those of CESCs. EESCs exhibited a significantly higher HGF gene expression compared with EuESCs (p < 0.05). Conclusions: These findings suggest reduced propensity to apoptosis and increased angiogenesis potential of EESCs, which may be involved in pathogenesis of endometriosis.

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Section: Discussionsupporting

confidence: 90%

Evaluation of apoptosis and angiogenesis in ectopic and eutopic stromal cells of patients with endometriosis compared to non-endometriotic controls

et al. 2020

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“…Resveratrol is considered to be effective in preventing and treating endometriosis. 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 In the present study, we showed that resveratrol inhibited the proliferation, migration, and invasion of ESCs in cell cultures ( Figures 4 A, 4C, and 4E) and suppressed the growth of ectopic endometrium in the mouse endometriosis model ( Figure 7 B). Then, we demonstrated that resveratrol decreased MTA1 expression and suppressed EMT induced by MTA1-ZEB2 in endometrial cells ( Figure 3 G) and endometriosis mice ( Figures 7 C and 7D).…”

Section: Discussionsupporting

confidence: 59%

“…Since EMT is involved in the pathogenesis of endometriosis, 2 , 22 , 23 , 24 , 25 , 26 , 27 , 28 we measured the expression of E-cadherin, an epithelial marker, and vimentin, a mesenchymal marker, by western blotting, and revealed lower E-cadherin expression and higher vimentin as well as MTA1 expression in EcESCs than those in NESCs ( Figures 3 A and 3D). Moreover, in a mouse endometriosis model, vimentin expression was higher and E-cadherin expression was lower in ectopic tissues than in eutopic tissues in IHC ( Figure 1 F).…”

Section: Resultsmentioning

confidence: 99%

“… 11 , 12 , 13 , 14 It is also effective in treating endometriosis. 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 Resveratrol not only increases antioxidant capacity and decreases lipid peroxidation, 15 but it also exhibits anti-inflammatory activity by suppressing nuclear factor κB (NF-kB), tumor necrosis factor (TNF)-α, and inflammatory cytokines in endometriosis. 20 More importantly, resveratrol has the activities of anti-proliferation and anti-invasion in endometriosis through inhibiting the expressions of VEGF, MMP-2, and MMP-9.…”

Section: Introductionmentioning

confidence: 99%

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MTA1, a Target of Resveratrol, Promotes Epithelial-Mesenchymal Transition of Endometriosis via ZEB2

Kong

Zhou

et al. 2020

Molecular Therapy - Methods & Clinical Development

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Endometriosis is a benign disease that shares some malignant features. Epithelial-mesenchymal transition (EMT) is involved in the pathogenesis of endometriosis. Metastasis-associated protein 1 (MTA1) plays an important role in various cancers by promoting EMT, yet there are no studies on its function in endometriosis. In the present study, we found that MTA1 was highly expressed in the ectopic endometrium of endometriosis patients and that the expression of MTA1 was related to the revised American Fertility Society stage. MTA1 facilitated endometrial stroma cell proliferation, migration, and invasion by inducing EMT, and the promotion function and MTA1 expression were suppressed by resveratrol, a natural polyphenol. Moreover, we revealed that MTA1 induced EMT through interaction with ZEB2. The findings in a mouse endometriosis model further showed that MTA1 and ZEB2 were upregulated in ectopic tissues and that resveratrol inhibited the growth of ectopic lesions and expression of MTA1 and ZEB2. Taken together, we demonstrate that MTA1 is a protein that promotes EMT via interacting with ZEB2 in the pathogenesis of endometriosis, and may be a target of resveratrol.

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“…In a pilot study, various concentrations of resveratrol (Sigma-Aldrich) (12.5, 25, 50, 100, 200 and 400 µmol/L) were tested to find safety dose of resveratrol by MTT assay, 17 based on MTT assay findings, 16 with EuESCs and CESCs (P < .01-<.0001; P < .0001; and P < .05-<.001, respectively). RANTES protein expression was also higher in…”

Section: Determining the Appropriate Concentration For Treatment Ofmentioning

confidence: 99%

Resveratrol treatment reduces expression of MCP‐1, IL‐6, IL‐8 and RANTES in endometriotic stromal cells

Kolahdouz‐Mohammadi

Shidfar

Khodaverdi

et al. 2020

J Cellular Molecular Medi

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Endometriosis is an enigmatic gynaecological disease characterized by ectopic implantation of endometrial like tissues in extra-uterine sites. 1 It afflicts approximately 10% of reproductive-aged women, 2%-11% of asymptomatic women, and almost half of the women experiencing chronic pelvic pain and associated with dysmenorrhoea, dyspareunia, pelvic pain and infertility. 1,2 The pathogenesis of endometriosis is not precisely understood. 3 Among numerous theories proposed to elucidate the pathophysiology of endometriosis, peritoneal implantation of viable endometrial cells during retrograde menstruation is the generally accepted one. 4 However, retrograde menstruation occurs in almost all reproductive-aged women, but only 10%-20% of them develop endometriosis, so other factors must be involved in implantation and survival of the displaced endometrial cells. 5 Based on recent findings, immune dysregulation

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Resveratrol reduces the expression of insulin‐like growth factor‐1 and hepatocyte growth factor in stromal cells of women with endometriosis compared with nonendometriotic women

Cited by 33 publications

References 53 publications

Evaluation of apoptosis and angiogenesis in ectopic and eutopic stromal cells of patients with endometriosis compared to non-endometriotic controls

Evaluation of apoptosis and angiogenesis in ectopic and eutopic stromal cells of patients with endometriosis compared to non-endometriotic controls

MTA1, a Target of Resveratrol, Promotes Epithelial-Mesenchymal Transition of Endometriosis via ZEB2

Resveratrol treatment reduces expression of MCP‐1, IL‐6, IL‐8 and RANTES in endometriotic stromal cells

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