Resveratrol suppresses the inflammatory responses of human gingival epithelial cells in a <scp>SIRT</scp>1 independent manner

Minagawa, Takayoshi; Okui, Takafumi; Takahashi, Naoki; Nakajima, Takako Eguchi; Tabeta, Koichi; Murakami, Shinya; Yamazaki, Kazuhisa

doi:10.1111/jre.12238

Cited by 27 publications

(23 citation statements)

References 37 publications

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“…This implies that the effect of resveratrol on inflammatory responses is mediated through a SIR1‐independent mechanism. In support of this idea, it has been demonstrated that resveratrol could suppress the inflammatory responses via NF‐κB, but not SIRT1 in human gingival epithelial and mouse embryonic fibroblasts [Kitada et al, ; Zagotta et al, ; Minagawa et al, ].…”

Section: Discussionmentioning

confidence: 99%

Resveratrol Ameliorates Palmitate-Induced Inflammation in Skeletal Muscle Cells by Attenuating Oxidative Stress and JNK/NF-κB Pathway in a SIRT1-Independent Mechanism

et al. 2017

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Resveratrol has been shown to exert anti-inflammatory and anti-oxidant effects in a variety of cell types, however, its role in prevention of inflammatory responses mediated by palmitate in skeletal muscle cells remains unexplored. In the present study, we investigated the effects of resveratrol on palmitate-induced inflammation and elucidated the underlying mechanisms in skeletal muscle cells. The results showed that palmitate significantly enhanced TNF-α and IL-6 mRNA expression and protein secretion from C2C12 cells at 12, 24, and 36 h treatments. Increased expression of cytokines was accompanied by an enhanced phosphorylation of JNK, P38, ERK1/2, and IKKα/IKKβ. In addition, JNK and P38 inhibitors could significantly attenuate palmitate-induced mRNA expression of TNF-α and IL-6, respectively, whereas NF-κB inhibitor reduced the expression of both cytokines in palmitate-treated cells. Resveratrol pretreatment significantly prevented palmitate-induced TNF-α and IL-6 mRNA expression and protein secretion in C2C12 cells. Importantly, pre-treatment of the cells with resveratrol completely abrogated the phosphorylation of ERK1/2, JNK, and IKKα/IKKβ in palmitate treated cells. The protection from palmitate-induced inflammation by resveratrol was accompanied by a decrease in the generation of reactive oxygen species (ROS). N-acetyl cysteine (NAC), a known scavenger of ROS, could protect palmitate-induced expression of TNF-α and IL-6. Furthermore, inhibition of SIRT1 by shRNA or sirtinol demonstrated that the anti-inflammatory effect of resveratrol in muscle cells is mediated through a SIRT1-independent mechanism. Taken together, these findings suggest that resveratrol may represent a promising therapy for prevention of inflammation in skeletal muscle cells. J. Cell. Biochem. 118: 2654-2663, 2017. © 2017 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

Resveratrol Ameliorates Palmitate-Induced Inflammation in Skeletal Muscle Cells by Attenuating Oxidative Stress and JNK/NF-κB Pathway in a SIRT1-Independent Mechanism

et al. 2017

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“…Interestingly, the level of this cytokine is significantly higher in gingival crevicular fluid from patients with chronic periodontitis than in healthy individuals, and decreases following periodontal treatments (Turer, Durmus, Balli, & Guven, 2017;Zhang, Chen, Zhu, & Yan, 2016). In recent studies, resveratrol was also found to decrease the production and expression of P. gingivalis LPS-induced proinflammatory cytokines in human periodontal ligament cells (Phoolcharoena et al, 2013;Rizzo et al, 2012) and human gingival epithelial cells (Minagawa et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Resveratrol attenuates the pathogenic and inflammatory properties of Porphyromonas gingivalis

Lagha

Andrian

Grenier

2019

Molecular Oral Microbiology

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Porphyromonas gingivalis has been strongly associated with chronic periodontitis, which affects tooth‐supporting tissues. This Gram‐negative anaerobic bacterium produces a repertoire of virulence factors that modulate tissue destruction directly or indirectly by the induction of inflammatory processes. The aim of this study was to investigate the effects of resveratrol, a major polyphenol found in grapes and wine, on the growth and virulence properties of P. gingivalis as well as on gingival keratinocyte tight junction integrity and the host inflammatory response. Resveratrol exhibited antibacterial activity that may result from damage to the bacterial cell membrane. Resveratrol also killed a pre‐formed P. gingivalis biofilm and reduced bacterial adherence to matrix proteins. In addition, resveratrol had a protective effect on the integrity of the keratinocyte tight junctions by inhibiting its breakdown by P. gingivalis. This may be related to the ability of resveratrol to inhibit the protease activities of P. gingivalis. Lastly, resveratrol reduced P. gingivalis‐mediated activation of the NF‐κB signaling pathway and attenuated TREM‐1 gene expression as well as soluble TREM‐1 secretion in monocytes. The effect on NF‐κB activation likely results from the ability of resveratrol to act as a PPAR‐γ agonist. In summary, the antibacterial, anti‐adherence, and antiprotease properties of resveratrol, as well as its ability to protect the gingival keratinocyte barrier and attenuate the inflammatory response in monocytes suggest that it may be a promising novel therapeutic agent for treating periodontal disease.

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“…It is found in grapes and red wine at concentrations of the μg/g and mg/glass order, respectively, and has been shown to exhibit anti‐inflammatory properties in IL‐1β‐stimulated epithelial cells or LPS‐stimulated periodontal ligament cells and rodent models (Baur et al., ; Donnelly et al., ; Pearson et al., ; Rizzo et al., ). We have previously shown that resveratrol strongly inhibits the inflammatory response in human gingival epithelial cells stimulated with Porphyromonas gingivalis by suppressing NF‐κB translocation (Minagawa et al., ). It has also been reported that resveratrol prevents the RANKL‐induced osteoclast differentiation of RAW 264.7 cells, possibly through suppression of reactive oxygen species production (He, Andersson, Lindgren, & Li, ).…”

Section: Introductionmentioning

confidence: 99%

Resveratrol suppresses the alveolar bone resorption induced by artificial trauma from occlusion in mice

et al. 2017

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Resveratrol suppresses the inflammatory responses of human gingival epithelial cells in a SIRT1 independent manner

Abstract: Resveratrol suppressed the inflammatory responses of P. gingivalis-stimulated HGECs, probably by inhibiting NF-κB signaling but independent of SIRT1.

Cited by 27 publications

References 37 publications

Resveratrol Ameliorates Palmitate-Induced Inflammation in Skeletal Muscle Cells by Attenuating Oxidative Stress and JNK/NF-κB Pathway in a SIRT1-Independent Mechanism

Resveratrol Ameliorates Palmitate-Induced Inflammation in Skeletal Muscle Cells by Attenuating Oxidative Stress and JNK/NF-κB Pathway in a SIRT1-Independent Mechanism

Resveratrol attenuates the pathogenic and inflammatory properties of Porphyromonas gingivalis

Resveratrol suppresses the alveolar bone resorption induced by artificial trauma from occlusion in mice

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