Ret finger protein mediates Pax7-induced ubiquitination of MyoD in skeletal muscle atrophy

Joung, Hosouk; Eom, Gwang Hyeon; Choe, Nakwon; Lee, Hye Mi; Ko, Jeong‐Hyeon; Kwon, Duk-Hwa; Nam, Yoon Seok; Min, Hyun-Ki; Shin, Sera; Kook, Jeewon; Cho, Young Kuk; Kim, Jeong Chul; Seo, Sang-Beom; Baik, Yung Hong; Nam, Kwang-Il; Kook, Hyun

doi:10.1016/j.cellsig.2014.07.006

Cited by 14 publications

(13 citation statements)

References 53 publications

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“…We provide evidence that Mdm2 modifies and targets C/EBP␤ for degradation by the 26 S proteasome and that this activity is required for myogenic differentiation. In addition to C/EBP␤, Pax7, Pax3, and MyoD are known to be regu- lated by the ubiquitin-proteasome system (27)(28)(29). However, while treatment with proteasome inhibitor protected C/EBP␤ protein levels in early differentiation, MyoD levels were not protected.…”

Section: Discussionmentioning

confidence: 81%

Mdm2 Promotes Myogenesis through the Ubiquitination and Degradation of CCAAT/Enhancer-binding Protein β

Lala-Tabbert

Lee

et al. 2015

Journal of Biological Chemistry

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Background: CCAAT/Enhancer-binding Protein ␤ (C/EBP␤) inhibits differentiation of muscle satellite cells and is rapidly down-regulated in early myogenesis. Results: The E3 ubiquitin ligase Mouse double minute 2 homolog (Mdm2) targets C/EBP␤ for degradation thereby promoting entry into myogenesis. Conclusion: Mdm2 expression is necessary for entry into myogenesis. Significance: Establishes a new role for Mdm2 in cellular differentiation.

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Section: Discussionmentioning

confidence: 81%

Mdm2 Promotes Myogenesis through the Ubiquitination and Degradation of CCAAT/Enhancer-binding Protein β

Lala-Tabbert

Lee

et al. 2015

Journal of Biological Chemistry

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“…Stuelsatz et al [ 19 ] reported that calpain 3 up-regulation negatively affects the differentiation process of C2C12 myoblast cells, and promotes generation of reserve cells in C2C12 myoblasts. Denervation injury reduced the MyoD protein amount [ 20 ]. Citing the views of related literature, our results showed an increasing level of CAPN3 expression during muscle degeneration phase, may inhibit myogenic differentiation; in this scenario, the down-regulation of CAPN3 during re-innervation could stimulate myogenic differentiation.…”

Section: Resultsmentioning

confidence: 99%

Calpain 3 Expression Pattern during Gastrocnemius Muscle Atrophy and Regeneration Following Sciatic Nerve Injury in Rats

Yan

Yao

et al. 2015

IJMS

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Calpain 3 (CAPN3), also known as p94, is a skeletal muscle-specific member of the calpain family that is involved in muscular dystrophy; however, the roles of CAPN3 in muscular atrophy and regeneration are yet to be understood. In the present study, we attempted to explain the effect of CAPN3 in muscle atrophy by evaluating CAPN3 expression in rat gastrocnemius muscle following reversible sciatic nerve injury. After nerve injury, the wet weight ratio and cross sectional area (CSA) of gastrocnemius muscle were decreased gradually from 1–14 days and then recovery from 14–28 days. The active form of CAPN3 (~62 kDa) protein decreased slightly on day 3 and then increased from day 7 to 14 before a decrease from day 14 to 28. The result of linear correlation analysis showed that expression of the active CAPN3 protein level was negatively correlated with muscle wet weight ratio. CAPN3 knockdown by short interfering RNA (siRNA) injection improved muscle recovery on days 7 and 14 after injury as compared to that observed with control siRNA treatment. Depletion of CAPN3 gene expression could promote myoblast differentiation in L6 cells. Based on these findings, we conclude that the expression pattern of the active CAPN3 protein is linked to muscle atrophy and regeneration following denervation: its upregulation during early stages may promote satellite cell renewal by inhibiting differentiation, whereas in later stages, CAPN3 expression may be downregulated to stimulate myogenic differentiation and enhance recovery. These results provide a novel mechanistic insight into the role of CAPN3 protein in muscle regeneration after peripheral nerve injury.

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“…MyoD, a key myogenic differentiation factor, contributes to muscle plasticity and regeneration (Joung et al, ) and regulates skeletal myogenesis (Han et al, ). The luciferase activity analysis as well as qPCR and Western blotting results showed that MyoD positively regulated FGF21 expression by binding to the FGF21 promoter region.…”

Section: Discussionmentioning

confidence: 99%

Fibroblast Growth Factor 21 (FGF21) Promotes Formation of Aerobic Myofibers via the FGF21‐SIRT1‐AMPK‐PGC1α Pathway

Liu

Wang

Hou

et al. 2017

Journal Cellular Physiology

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Fibroblast growth factor 21(FGF21) is a pivotal regulator of energy metabolism, which is currently being assessed as a potential drug target for the treatment of insulin-resistant conditions. However, the cellular mechanisms by which FGF21 affects myogenesis remain unclear. In this study, we explored the function of FGF21 in myogenesis both in vitro and in vivo. Our experiments showed for the first time that FGF21 promotes myoblast differentiation and serves as a switch of molecular transformation from anaerobic myofibers to aerobic myofibers via the FGF21-SIRT1-AMPK-PGC1α axis. Furthermore, we employed the Dual-Luciferase Reporter Assay System and Electrophoretic Mobility Shift Assay (EMSA) and demonstrated that MYOD, a major myogenic transcription factor, binds directly to the promoter region of Fgf21, leading to the activation of Fgf21 expression in mouse C2C12 myoblasts. Our study revealed a novel mechanism of myogenesis and muscle fiber transformation and indicated that FGF21 serves as a vital regulator of muscle development and important contributor to the pathogenesis of myopathy. J. Cell. Physiol. 232: 1893-1906, 2017. © 2016 Wiley Periodicals, Inc.

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Ret finger protein mediates Pax7-induced ubiquitination of MyoD in skeletal muscle atrophy

Cited by 14 publications

References 53 publications

Mdm2 Promotes Myogenesis through the Ubiquitination and Degradation of CCAAT/Enhancer-binding Protein β

Mdm2 Promotes Myogenesis through the Ubiquitination and Degradation of CCAAT/Enhancer-binding Protein β

Calpain 3 Expression Pattern during Gastrocnemius Muscle Atrophy and Regeneration Following Sciatic Nerve Injury in Rats

Fibroblast Growth Factor 21 (FGF21) Promotes Formation of Aerobic Myofibers via the FGF21‐SIRT1‐AMPK‐PGC1α Pathway

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