Retained integrity of protein encapsulated in spray-dried chitosan microparticles

Drug Carrier Systems Using Chitosan for Non Parenteral Routes

“…This difference can be attributed to difference in solubility of chitosans [108]. Similar results were reported in other studies [109][110][111][112].…”

Supercritical fluid assisted production of micrometric powders of the labile trypsin and chitosan/trypsin composite microparticles

“…According to the measurement, all the loading capacities of the samples prepared under different polymer/protein ratios were quite close to the theoretical values calculated from the corresponding feed ratios. In general, trypsin was successfully entrapped into the chitosan microparticles with high loading efficiencies of above 85% which were comparable to some studies on spray-dried chitosan/protein microparticles (Kusonwiriyawong et al, 2009), implying that most of the protein was incorporated into the composite particles. Therefore, SAA-HCM process could obtain a superior protein loading efficiency when preparing polymer/protein coprecipitates from aqueous solution.…”

“…Besides, the increase of the protein content could impair the rigidity, resulting the aggravated winkles. Semblable phenomenon was also reported when preparing spray-dried polymer/protein composite microparticles (Kusonwiriyawong et al, 2009).…”

Preparation and characterization of chitosan microsphere loading bovine serum albumin

“…Chitosan microspheres/nanoparticles have been prepared by different methods such as ioncrosslinking, ionic/covalent crosslinking [14][15][16] , chemical crosslinking [17] , membrane emulsifi cation technique [18] , spray-drying technique [19] , inverse phase emulsion dispersion method [20] , compounding strategies such as modified ionotropic gelation method combined with a high voltage electrostatic field [21] and there are many reports on the uses of chitosan microspheres or nanoparticles as carriers as protein [15,16,[21][22][23][24][25][26][27][28] , and DNA delivery systems [24] . In addition, the modifi ed chitosan hydrogel with pH-sensitive characteristics has also been developed for controlling the release of protein molecules [23,29,30] .…”

“…In addition, the modifi ed chitosan hydrogel with pH-sensitive characteristics has also been developed for controlling the release of protein molecules [23,29,30] . In the protein delivery system, bovine serum albumin (BSA) is the most frequently used model protein molecules [19,30,31] . Recently, the chitosan-BSA system has been designed as model for isolation of protein from the solution as this system does not change the secondary or tertiary structure of the protein molecules [32] .…”