Retension mechanism in reversed-phase ion-pair chromatography of amines and amino acids on bonded phases

Deelder, R.S.; Linssen, H.A.J.; Konunenduk, A.P.; Venne, J.L.M. van de

doi:10.1016/s0021-9673(00)85607-1

Cited by 84 publications

(12 citation statements)

References 26 publications

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“…These observations are consistent with the literature where retention time reduction was reported as increasing with the ion-pairing reagent concentration (3)(4)(5)10,11). Several possible mechanisms, including micelle formation of the ion-pairing reagent, desolvation, and counter ions from the ion-pairing reagent, have been proposed in these papers.…”

Section: Effect Of the Tba Concentration On Retentionsupporting

confidence: 93%

“…The retention mechanism in ion-pair chromatography is based on the interactions formed between the ionic solutes and ion-pairing reagent adsorbed on stationary phase (1). The retention process, which is affected by both ion exchange with the counter ions and adsorption of the ionic solutes on the charged stationary phase, is primarily a function of the ion-pairing reagent concentration on the stationary phase (2)(3)(4)(5)(6). Numerous studies have evaluated parameters which affect ion-pair reagent adsorption behaviors (7)(8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

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Regeneration of Tetrabutylammonium Ion-Pairing Reagent Distribution in a Gradient Elution of Reversed Phase Ion-Pair Chromatography

Zhang

Raglione

Wang

et al. 2011

Journal of Chromatographic Science

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The regeneration of ion-pairing reagent distribution on liquid chromatography columns after gradient elution has been well recognized as the cause for long column equilibration time, a major drawback associated with gradient elution reverse phase ion-pair chromatography. To date, the majority of studies have focused on optimizing the separation conditions to shorten the equilibration time. There is limited understanding of the ion-pairing reagent distribution process between the mobile phase and stationary phase in the course of gradient elution, and subsequent column re-equilibration. The focus of this work is to gain a better understanding of this process. An ion-pair chromatographic system, equipped with a YMC ODS C(18) column and a mobile phase containing tetrabutylammonium (TBA) hydroxide as the ion-pairing reagent, was used in the study. The TBA distribution profile was established by measuring its concentration in the eluent fractions collected during the gradient cycle using different column equilibration times with an ion chromatographic method. Furthermore, the analyte retention time was evaluated as the function of the column equilibration time and TBA concentration in the mobile phase. The column equilibration and its impact on the method robustness will also be discussed.

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Section: Effect Of the Tba Concentration On Retentionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Regeneration of Tetrabutylammonium Ion-Pairing Reagent Distribution in a Gradient Elution of Reversed Phase Ion-Pair Chromatography

Zhang

Raglione

Wang

et al. 2011

Journal of Chromatographic Science

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“…The only theoretical model for the IIC of zwitterions is a stoichiometric one, and it was developed by Deelder and co-workers. 17 We have already discussed the limits of the stoichiometric approach. 11 Whereas the retention equation of this model predicts that the dipolar analyte will not be retained in the absence of H, experimental works including the present one demonstrate that this is obviously not true.…”

Section: Introductionmentioning

confidence: 99%

Ion-interaction chromatography of zwitterions. The fractional charge approach to model the influence of the mobile phase concentration of the ion-interaction reagent

Cecchi¹,

Pucciarelli

Passamonti

2004

Analyst

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“…alkylsulfonic acid sodium salts, quaternary ammonium salts) are commonly used as ion‐pairing agents, added to mobile phase, to promote ionic analyte interaction with hydrophobic stationary phase. A negatively charged reagent having a charge opposite to the analyte of interest, such as sodium alkyl‐sulfonate, can be uset to retain positively charged ionic bases (Deelder et al ., 1979; Fan and Stewart, 2002). Similary, a positively charged reagent, such as tetrabutyl ammonium chloride, can be used to retain negatively charged ionic acids.…”

Section: Introductionmentioning

confidence: 99%

Retention study of some cation‐type compounds using bile acid sodium salts as ion pairing agents in liquid chromatography

Radulescu

Voicu

Medvedovici

et al. 2010

Biomedical Chromatography

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The possibility of forming ion-pairs between bile acids (sodium taurocholate, sodium taurodeoxycholate and sodium taurochenodeoxycholate) and different compounds (pralidoxime, obidoxime and pyridostigmine) having a cationic character has been studied in reversed-phase liquid chromatography (RP-LC). This study can be useful in understanding the role of bile acids in the transport of ionic species through hydrophobic membrane. The present study focused on the influence of mobile phase composition on the retention parameters of chosen compounds (percentage of acetonitrile, pH of aqueous component or ionic strength). For constant concentration of bile acids in aqueous component of mobile phase the functional dependencies between the logarithm of the retention factor (k) and the methanol content in the mobile phase followed a binomial pattern (U-shaped), with a minimum positioned within the interval 70-85% methanol.

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Retension mechanism in reversed-phase ion-pair chromatography of amines and amino acids on bonded phases

Cited by 84 publications

References 26 publications

Regeneration of Tetrabutylammonium Ion-Pairing Reagent Distribution in a Gradient Elution of Reversed Phase Ion-Pair Chromatography

Regeneration of Tetrabutylammonium Ion-Pairing Reagent Distribution in a Gradient Elution of Reversed Phase Ion-Pair Chromatography

Ion-interaction chromatography of zwitterions. The fractional charge approach to model the influence of the mobile phase concentration of the ion-interaction reagent

Retention study of some cation‐type compounds using bile acid sodium salts as ion pairing agents in liquid chromatography

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