Retention Behavior of Polycyclic Aromatic Compounds in a Novel Polymer-Based Stationary Phase Liquid Chromatography

Abstract

A novel polymer-based particles was introduced as a stationary phase in liquid chromatography (LC). Spherical particles of a copolymer, poly(benzoguanamine-co-formaldehyde) (BF), was packed into a conventional blank LC column and the retention behavior of polycyclic aromatic compounds (PACs) was evaluated in reversed-phase LC. The trend for the retention was compared with that obtained on commercially-available octadecylsilica (ODS) and phenylbutylsilica (PBS) phases. In terms of the trend on the retentivity for PACs, the BF phase showed a similar behavior as that on the ODS phase, although the selectivity to some critical pairs was somewhat different from that on the ODS phase. The results suggest that the BF phase has a retentivity basically on the basis of hydrophobic interaction between the stationary phase ligands and the solute molecule, however, at the same time, the BF phase has a different selectivity from the PBS phase for the recognition of PACs molecules. On the basis of the comparison with the PBS phase, the unique selectivity of the BF phase can be attributed to the restricted moving of the phenyl ligands.

Retention Behavior of Various Aromatic Compounds on Poly(butylene terephthalate) Stationary Phase in Liquid Chromatography

“…As majority of the LC separations are carried out using a reversed-phase condition [9], development of chemically modified silica stationary phase is regarded as a key step for improving the separation of complex sample matrices consisted of many components [9][10][11][12][13][14].…”

“…Development of novel stationary phases, especially polymer-based stationary phase, has also been desired to create more efficient and cost-effective separations in chromatography [10,40]. Taking advantage of a good stability to a mobile phase of highly-acidic and highly-basic conditions, various applications have been reported [40].…”

Spherical Polyimide Particles as a Novel Stationary Phase in Liquid Chromatography

“…The trend in Fig. 4a is somewhat different from that on a phenylbutyl bonded silica (PBS) phase [26].…”

“…In terms of the use of polymer-based materials in LC, spherical particles of the various polymers and copolymers have been mainly studied as the stationary phase, although fibrous polymeric materials were also introduced as a stationary phase in gas chromatography [4][5][6][7][8][9][10][11][12][13][14] and LC [13][14][15][16][17][18][19][20][21][22][23][24][25][26], along with an extraction medium in sample preparation techniques for liquid samples [27][28][29][30][31][32][33][34][35][36][37][38][39] and gas samples [40][41][42][43][44][45]. In our previous investigation, a spherical polyimide (PI) particles have been introduced as a novel extraction medium in miniaturized sample preparation technique [45,46].…”

Ultralow-Temperature HPLC Using Low-Molecular-Weight Hydrocarbons as Mobile Phases

“…Chromatography is not only useful for the separation of mixtures, it can also be used to study interactions between the stationary phase and compounds [9][10][11]. The development of chromatography under cryogenic conditions will allow to investigate interactions between compounds at ultralow-temperatures, and will contribute to understanding the chemistry at cryogenic conditions.…”