Retention Mechanisms in Different HPLC Types

Moldoveanu, Serban C.; David, Víctor

doi:10.1016/b978-0-12-385013-3.00005-7

Cited by 8 publications

(4 citation statements)

References 49 publications

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“…Clearance (CL) was calculated using Equation 13and the apparent volume of distribution of the elimination phase (V d ) were calculated using Equation (14). The tissue to blood (Kp) ratio was calculated by using Equation (15…”

Section: Discussionmentioning

confidence: 99%

“…Because we used a reversed-phase stationary we were able to employ a polar mobile system consists of an isocratic elution of 0.1% AA in water (10% mobile phase A) and MeOH (90% mobile phase B). The use of the polar mobile system resulted in acceptable separation with high peak efficiency, peak symmetry, and short total run time of 6 min, making the method time efficient [ 11 , 12 , 15 , 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…The developed SPE protocol was created while considering the drug physical-chemical properties and compatibility with the chosen LC method. The extraction method was reproducible with consistent high extraction recovery averaged at >90% of MP1 and >85% of IS, and negligible matrix effect [ 15 , 16 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

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Assessment of Tissue Distribution and Metabolism of MP1, a Novel Pyrrolomycin, in Mice Using a Validated LC-MS/MS Method

et al. 2020

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MP1 is a novel marinopyrrole analogue with activity in MYCN amplified neuroblastoma cell lines. A rapid, selective, and sensitive liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method was developed and validated for quantitation of MP1 in mouse plasma. Analyte separation was achieved using a Waters Acquity UPLC®BEH C18 column (1.7 µm, 100 × 2.1 mm). Mobile phase consisted of 0.1% acetic acid in water (10%) and methanol (90%) at a total flow rate of 0.25 mL/min. The mass spectrometer was operated at unit resolution in the multiple reaction monitoring (MRM) mode, using precursor ion > product ion transitions of 324.10 > 168.30 m/z for MP1 and 411.95 > 224.15 m/z for PL-3. The MS/MS response was linear over the concentration range from 0.2–500 ng/mL for MP1, correlation coefficient (r2) of 0.988. Precision (% RSD) and accuracy (% bias) were within the acceptable limits as per FDA guidelines. MP1 was stable under storage and laboratory handling conditions. The validated method was successfully applied to assess the solubility, in-vitro metabolism, plasma protein binding, and bio-distribution studies of MP1.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Assessment of Tissue Distribution and Metabolism of MP1, a Novel Pyrrolomycin, in Mice Using a Validated LC-MS/MS Method

et al. 2020

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“…The retention mechanism in IP-RP chromatography is very complex and governed by a number of factors [ 9 , 10 ]. In one of the models, the IPR and the oppositely-charged solute molecule interact with each other electrostatically in the mobile phase to form a neutral complex which then interacts hydrophobically with the nonpolar stationary phase [11][12][13] . In another model, the IPR in the mobile phase first gets adsorbed to the nonpolar stationary phase, through the hydrophobic interaction of the alkyl side chain of the IPR.…”

Section: Introductionmentioning

confidence: 99%