Rethinking water treatment targets: Bacteria regrowth under unprovable conditions

Moreira, Nuno F.F.; Ribeirinho-Soares, Sara; Viana, Ana Teresa; Graça, Cátia A.L.; Ribeiro, Ana R.; Castelhano, Nadine; Egas, Conceição; Pereira, M.F.R.; Silva, Adrián M.T.; Nunes, Olga C.

doi:10.1016/j.watres.2021.117374

Cited by 22 publications

(14 citation statements)

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“…2 a). Proteobacteria selection was also observed after ozonation of municipal wastewater ( Moreira et al, 2021 ) and suggested higher resistance of this group to oxidative conditions. The bacterial community present in MWWTPE is sensitive to H 2 O 2 , which can function either as a disinfectant ( Apel and Hirt, 2004 ) or as an oxygen source enhancing aerobic bacterial growth ( Hinchee et al, 1991 ; Zappi et al, 2000 ).…”

Section: Resultsmentioning

confidence: 95%

“…In contrast, recent literature points out to the spread and selection of ARB and ARGs after the application of traditional technologies such as Chlorine, ozonation and UV-C irradiation/oxidant ( Jin et al, 2020 ; Kirchner et al, 2020 ; Lee et al, 2021 ; Moreira et al, 2021 ; Sharma et al, 2019 ). In addition, ozonation and UV-C irradiated processes are energy intensive and costly, which may hinder their application in developing countries.…”

Section: Resultsmentioning

confidence: 98%

“…Chlorination may select ARB favoring their spread and affects intra and extracellular concentrations of ARGs ( Guo et al, 2015 ; Hou et al, 2019 ; Liu et al, 2018 ). Recently, ozonation has been associated with the selection of Pseudomonas aeruginosa ( Moreira et al, 2021 ), a priority pathogen according to the WHO (2017) . In contrast, Advanced Oxidation Processes (AOP) are feasible methods for the inactivation of bacteria and elimination of ARGs as oxidative radicals damage cell membrane and DNA structure through free radical reactions ( Guo et al, 2020 ; Li et al, 2021 ; Michael-Kordatou et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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Metagenomic analysis of MWWTP effluent treated via solar photo-Fenton at neutral pH: Effects upon microbial community, priority pathogens, and antibiotic resistance genes

Vilela

Neto

Starling

et al. 2021

Science of The Total Environment

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The effectiveness of advanced technologies on eliminating antibiotic resistant bacteria (ARB) and resistance genes (ARGs) from wastewaters have been recently investigated. Solar photo-Fenton has been proven effective in combating ARB and ARGs from Municipal Wastewater Treatment Plant effluent (MWWTPE). However, most of these studies have relied solely on cultivable methods to assess ARB removal. This is the first study to investigate the effect of solar photo-Fenton upon ARB and ARGs in MWWTPE by high throughput metagenomic analysis (16S rDNA sequencing and Whole Genome Sequencing). Treatment efficiency upon priority pathogens and resistome profile were also investigated. Solar photo-Fenton (30 mg L −1 of Fe 2+ intermittent additions and 50 mg L −1 of H 2 O 2 ) reached 76–86% removal of main phyla present in MWWTPE. An increase in Proteobacteria abundance was observed after solar photo-Fenton and controls in which H 2 O 2 was present as an oxidant (Fenton, H 2 O 2 only, solar/H 2 O 2 ). Hence, tolerance mechanisms presented by this group should be further assessed. Solar photo-Fenton achieved complete removal of high priority Staphylococcus and Enterococcus , as well as Klebsiella pneumoniae and Pseudomonas aeruginosa . Substantial reduction of intrinsically multi-drug resistant bacteria was detected. Solar photo-Fenton removed nearly 60% of ARGs associated with sulfonamides, macrolides, and tetracyclines, and complete removal of ARGs related to β-lactams and fluoroquinolones. These results indicate the potential of using solar-enhanced photo-Fenton to limit the spread of antimicrobial resistance, especially in developing tropical countries.

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Metagenomic analysis of MWWTP effluent treated via solar photo-Fenton at neutral pH: Effects upon microbial community, priority pathogens, and antibiotic resistance genes

Vilela

Neto

Starling

et al. 2021

Science of The Total Environment

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“…All the disinfection processes reviewed in this study have the potential to select bacteria harboring iARGs and result in increased relative abundances of iARGs. ,,,,− For instance, the relative abundance of antibiotic efflux pump genes remarkably increased from 0.13 ± 0.01 to 0.26 ± 0.01 after chlorination in a full-scale drinking water treatment plant . Bacteria in secondary effluent surviving ozonation was found to be highly resistant to antibiotics, indicating the selection effect of ozone. , In a full-scale WWTP employing ClO 2 disinfection (ClO 2 8–9 mg/L, 30 min), increases of up to 173.3-fold in the relative abundances of iARGs were observed . Similarly, the total relative abundance of the 15 detected iARGs increased from 3.2 × 10 –5 to 1.9 × 10 –4 with UV irradiation at 500 mJ/cm 2 …”

Section: Redistribution Of Iargs and Eargs In Disinfection Processesmentioning

confidence: 94%

“…43 Bacteria in secondary effluent surviving ozonation was found to be highly resistant to antibiotics, indicating the selection effect of ozone. 143,144 In a full-scale WWTP employing ClO 2 disinfection (ClO 2 8−9 mg/ L, 30 min), increases of up to 173.3-fold in the relative abundances of iARGs were observed. 44 Similarly, the total relative abundance of the 15 detected iARGs increased from 3.2 × 10 −5 to 1.9 × 10 −4 with UV irradiation at 500 mJ/cm 2 .…”

Section: Disinfectants and Dbps Canmentioning

confidence: 99%

Elimination and Redistribution of Intracellular and Extracellular Antibiotic Resistance Genes in Water and Wastewater Disinfection Processes: A Review

Liu

et al. 2022

ACS EST Water

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Antibiotic resistance genes (ARGs) have been identified as emerging contaminants in water and wastewater. Concerns have been raised of whether disinfection processes can effectively eliminate ARGs and thus mitigate their spread. Due to the different existing forms and dissemination pathways, intracellular ARGs (iARGs) and extracellular ARGs (eARGs) have different responses to disinfection processes. This review compares the elimination of iARGs and eARGs in representative disinfection processes applied at water and wastewater treatment plants, including treatment by chlorine, chloramine, chlorine dioxide, ozone, ultraviolet irradiation (UV), and combination of UV with chemicals. Meanwhile, the redistribution of ARGs upon disinfection processes is highlighted. Elimination efficiencies of ARGs generally follow the order: ozone > chlorine ≫ chlorine dioxide > chloramine. The elimination of naked eARGs could be up to 4-log higher than that of iARGs. Notably, these disinfectants and the resulting disinfection byproducts formed in disinfection process can select antibiotic resistant bacteria (ARB) and increase relative abundances of iARGs. Under practical disinfectant exposure, elevated abundances of eARGs may be observed. Low doses of chlorine and chloramine tend to enhance the transfer of ARGs from intracellular to extracellular forms. To selectively control ARGs and associated risks and meanwhile minimize the unintended consequences, the disinfection practice may warrant optimization.

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The Complex Interplay Between Antibiotic Resistance and Pharmaceutical and Personal Care Products in the Environment

Manaia

Aga

Cytryn

et al. 2023

Enviro Toxic and Chemistry

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Antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are important environmental contaminants. Nonetheless, what drives the evolution, spread, and transmission of antibiotic resistance dissemination is still poorly understood. The abundance of ARB and ARGs is often elevated in human-impacted areas, especially in environments receiving fecal wastes, or in the presence of complex mixtures of chemical contaminants, such as pharmaceuticals and personal care products. Self-replication, mutation, horizontal gene transfer, and adaptation to different environmental conditions contribute to the persistence and proliferation of ARB in habitats under strong anthropogenic influence. Our review discusses the interplay between chemical contaminants and ARB and their respective genes, specifically in reference to co-occurrence, potential biostimulation, and selective pressure effects, and gives an overview of mitigation by existing man-made and natural barriers. Evidence and strategies to improve the assessment of human health risks due to environmental antibiotic resistance are also discussed.

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Rethinking water treatment targets: Bacteria regrowth under unprovable conditions

Cited by 22 publications

References 93 publications

Metagenomic analysis of MWWTP effluent treated via solar photo-Fenton at neutral pH: Effects upon microbial community, priority pathogens, and antibiotic resistance genes

Metagenomic analysis of MWWTP effluent treated via solar photo-Fenton at neutral pH: Effects upon microbial community, priority pathogens, and antibiotic resistance genes

Elimination and Redistribution of Intracellular and Extracellular Antibiotic Resistance Genes in Water and Wastewater Disinfection Processes: A Review

The Complex Interplay Between Antibiotic Resistance and Pharmaceutical and Personal Care Products in the Environment

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