Retinal expression, regulation, and functional bioactivity of prostacyclin-stimulating factor

Glioblastoma-secreted factors induce IGFBP7 and angiogenesis by modulating Smad-2-dependent TGF-β signaling

“…IGFBP7 expression in peripheral endothelial cells can be modulated by cell confluence, hypoxia, and cytokines including VEGF, bFGF and TGF-b1 (Hata et al, 2000). In this study, IGFBP7 expression in HBEC was potently induced by U87MG-CM and TGF-b1 and was not affected by VEGF or hypoxia.…”

“…Either VEGF-A or SPARC (20 ng/ml) demonstrated minimal effects on IGFBP7 expression in HBEC (A Pen, unpublished). On the basis of previous studies showing that TGF-b1 induces IGFBP7 expression in bovine retinal capillary endothelial cells (Hata et al, 2000), we examined whether TGFb1 is released in U87MG-CM. The average amount of TGF-b1 secreted in U87MG-CM determined by ELISA was B5 pM.…”

A High Endothelial Venule Secretory Protein, Mac25/Angiomodulin, Interacts with Multiple High Endothelial Venule-Associated Molecules Including Chemokines

“…Functionally, mac25/AGM has been implicated in various biological responses: it accumulates in the blood vessels of tumors (10), preferentially binds to extracellular matrix (ECM) components such as collagen type IV (10), promotes cell spreading of the human bladder carcinoma cell line ECV-304 on collagen type IV (10), and stimulates PGI 2 production, which is a potent vasodilator (13). It has been reported that mac25/AGM increases PGI 2 production in retinal endothelial cells, resulting in an increased retinal blood flow in vivo (14). In addition, mac25/AGM binds to heparan sulfate (HS) glycosaminoglycans (GAGs) (11,12) and has been implicated in angiogenesis (10,11).…”

Growth factors and protein kinase C inhibitors as novel therapies for the medical management diabetic retinopathy

“…Those that have received considerable evaluation include basic fibroblastic growth factor (bFGF), growth hormone (GH), 25,26 insulin-like growth factor -1 (IGF-1), 27,28 connective tissue growth factor (CTGF), 29 prostacyclin-stimulating factor (PSF), 30 hepatocyte growth factor (HGF), [31][32][33] and vascular endothelial growth factor (VEGF). 34,35 bFGF, GH, IGF-1, and CTGF are growth factors that do not fulfil all of the Michaelson criteria, but probably have either synergistic or permissive roles in PDR.…”

“…38 Another factor, PSF, stimulates endothelial cell synthesis of prostacyclin PG12, a vasoactive prostaglandin, which may be responsible for the biphasic changes in retinal blood flow seen in diabetes. 39 HGF has attributes consistent with a mediator of proliferative retinopathy, but its role is still under investigation. HGF is a pleiotropic molecule and its receptor, c-Met, like that of VEGF's KDR receptor, is a transmembrane tyrosine kinase that is autophosphorylated in response to HGF binding.…”