Retinal Pigment Epithelium: Proliferation and Differentiation during Development and Regeneration

Stroeva, O. G.; Mitashov, V. I.

doi:10.1016/s0074-7696(08)61689-7

Cited by 140 publications

(65 citation statements)

References 116 publications

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“…This is consistent with the conventional idea of how the RPE grows. The central area differentiates earlier than the marginal zones (Stroeva and Mitashov, 1983). The proliferative state of the RPE has, however, led to debates (reviewed by Stroeva and Mitashov, 1983).…”

Section: Complementary Expression Of Hh Pathway Genes In the Central mentioning

confidence: 99%

“…The central area differentiates earlier than the marginal zones (Stroeva and Mitashov, 1983). The proliferative state of the RPE has, however, led to debates (reviewed by Stroeva and Mitashov, 1983). In chick, it was assumed that mitotic activity had ceased completely in the RPE of a 4-day-old embryo, while others have found mitosis later in the embryonic RPE, but it was thought that by day 14, there were no dividing cells in the RPE.…”

Section: Complementary Expression Of Hh Pathway Genes In the Central mentioning

confidence: 99%

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A novel function forHedgehogsignalling in retinal pigment epithelium differentiation

et al. 2003

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Sonic hedgehog is involved in eye field separation along the proximodistal axis. We show that Hh signalling continues to be important in defining aspects of the proximodistal axis as the optic vesicle and optic cup mature. We show that two other Hedgehog proteins, Banded hedgehog and Cephalic hedgehog, related to the mouse Indian hedgehog and Desert hedgehog, respectively, are strongly expressed in the central retinal pigment epithelium but excluded from the peripheral pigment epithelium surrounding the ciliary marginal zone. By contrast, downstream components of the Hedgehog signalling pathway, Gli2, Gli3 and X-Smoothened, are expressed in this narrow peripheral epithelium. We show that this zone contains cells that are in the proliferative state. This equivalent region in the adult mammalian eye, the pigmented ciliary epithelium, has been identified as a zone in which retinal stem cells reside. These data, combined with double labelling and the use of other retinal pigment epithelium markers, show that the retinal pigment epithelium of tadpole embryos has a molecularly distinct peripheral to central axis. In addition, Gli2, Gli3 and X-Smoothened are also expressed in the neural retina, in the most peripheral region of the ciliary marginal zone, where retinal stem cells are found in Xenopus, suggesting that they are good markers for retinal stem cells. To test the role of the Hedgehog pathway at different stages of retinogenesis, we activated the pathway by injecting a dominant-negative form of PKA or blocking it by treating embryos with cyclopamine. Embryos injected or treated at early stages display clear proximodistal defects in the retina. Interestingly, the main phenotype of embryos treated with cyclopamine at late stages is a severe defect in RPE differentiation. This study thus provides new insights into the role of Hedgehog signalling in the formation of the proximodistal axis of the eye and the differentiation of retinal pigment epithelium.

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Section: Complementary Expression Of Hh Pathway Genes In the Central mentioning

confidence: 99%

Section: Complementary Expression Of Hh Pathway Genes In the Central mentioning

confidence: 99%

A novel function forHedgehogsignalling in retinal pigment epithelium differentiation

et al. 2003

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“…In tailed amphibian (Urodela) complete removal of NR or NR artificial detachment leads to RPE cell transdifferentiation that two months later results in regeneration of functioning retina (Chiba & Mitashov, 2007;Grigoryan & Mitashov, 1979;Hasegawa, 1958;Mitashov, 1997;Stroeva & Mitashov, 1983). In mammals RPE-based NR regeneration has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Shared Triggering Mechanisms of Retinal Regeneration in Lower Vertebrates and Retinal Rescue in Higher Ones

Grigoryan¹

2012

Tissue Regeneration - From Basic Biology to Clinical Application

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“…Neither RPE, nor neuroretinal tissue on their own are able to develop into stratospheroids. RPE, which is implicated in retinal regeneration (see Stroeva & Mitashov 1983;Hitchcock & Raymond 1992;Layer & Willbold 1993; see also ½ 4), seems to play a major role in retinogenesis. For instance, RPE explanted on a nitrocellulose ¢lter strongly promotes cell proliferation and tissue lamination of a retinal explant which is grown on top of it (Liu et al 1988).…”

Section: Introductionmentioning

confidence: 99%

Pigmented epithelium induces complete retinal reconstitution from dispersed embryonic chick retinae in reaggregation culture

Rothermel

Willbold

DeGrip

et al. 1997

Proc. R. Soc. Lond. B

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SU M M A RYReaggregation of dispersed retinal cells of the chick embryo leads to histotypic retinospheroids in which the laminar organization remains incomplete: photoreceptors form rosettes which are surrounded by constituents of the other retinal layers. Here, for the ¢rst time, a complete arrangement of layers is achieved in cellular spheres (stratoids), provided that fully dispersed retinal cells are younger than embryonic day E6, and are reaggregated in the presence of a monolayer of retinal pigmented epithelium (RPE). A remarkable mechanism of stratoid formation from 1 to 15 days in vitro is revealed by the establishment of a radial MÏller glia sca¡old and of photoreceptors. During the ¢rst two days of reaggregation on RPE, rosettes are still observed. At this stage immunostaining with vimentin and F11 antibodies for radial MÏller glia reveal a disorganized pattern. Subsequently, radial glia processes organize into long parallel ¢bre bundles which are arranged like spokes to stabilize the surface and centre of the stratoid. The opsin-speci¢c antibody CERN 901 detects photoreceptors as they gradually build up an outer nuclear layer at the surface. These ¢ndings assign to the RPE a decisive role for the genesis and regeneration of a vertebrate retina.

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Retinal Pigment Epithelium: Proliferation and Differentiation during Development and Regeneration

Cited by 140 publications

References 116 publications

A novel function forHedgehogsignalling in retinal pigment epithelium differentiation

A novel function forHedgehogsignalling in retinal pigment epithelium differentiation

Shared Triggering Mechanisms of Retinal Regeneration in Lower Vertebrates and Retinal Rescue in Higher Ones

Pigmented epithelium induces complete retinal reconstitution from dispersed embryonic chick retinae in reaggregation culture

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