Retinal Regeneration Following OCT-Guided Laser Injury in Zebrafish

DiCicco, Rose M; Bell, Brent A.; Kaul, Charles; Hollyfield, Joe G.; Anand‐Apte, Bela; Tao, Yuankai K.; Yuan, Alex

doi:10.1167/iovs.14-14724

Cited by 27 publications

(26 citation statements)

References 16 publications

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“…Animals were maintained at 28.5°C on a 14-hour light/10-hour dark cycle according to standard procedures (Westerfield, 2007). Zebrafish imaging methods and animal preparatory procedures have been previously reported in detail (Bell et al, 2014; DiCicco et al, 2014). All experiments utilized light-adapted animals.…”

Section: Methodsmentioning

confidence: 99%

“…The details of the HRA2 SLO system (Heidelberg Engineering USA, Inc.) modified and applied to zebrafish imaging have been previously published (Bell et al, 2014; DiCicco et al, 2014). When using the HRA2 with the 55° wide field lens, the z-module setting can be adjusted to provide a focus range more suitable for imaging zebrafish (Suppl.…”

Section: Methodsmentioning

confidence: 99%

“…SLO, introduced for patient use in the early ‘90s, has become an important diagnostic imaging tool for ophthalmologists (Hassenstein and Meyer, 2009). Over the past decade this instrument has found widespread use in non-clinical research for imaging numerous animal models such as non-human primates (Rosolen et al, 2001), canines (Rosolen et al, 2001), swine (Rosolen et al, 2001), rodents (Hossain et al, 1998; Seeliger et al, 2005), and fish (DiCicco et al, 2014; Hossain et al, 1998; Rosolen et al, 2001; Seeliger et al, 2005). SLO has several operating modes and wavelength combinations that provide some advantages over broadband visible light fundus imaging (Bell et al, 2014), including the ability to trace fluorescent tagged cells or proteins in vivo .…”

Section: Introductionmentioning

confidence: 99%

“…We have previously demonstrated that in vivo transpupillary imaging of the adult zebrafish retina ( Danio rerio ) is feasible using commercially available, standalone SLO and SDOCT imaging systems (Bell et al, 2014; DiCicco et al, 2014). More specifically, this work focused on quantifying vascular leakage and laser-induced thermal injury.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The adult zebrafish retina: In vivo optical sectioning with Confocal Scanning Laser Ophthalmoscopy and Spectral-Domain Optical Coherence Tomography

Bell

Yuan

DiCicco

et al. 2016

Experimental Eye Research

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Non-invasive imaging is an invaluable diagnostic tool in ophthalmology. Two imaging devices, the scanning laser ophthalmoscope (SLO) and spectral domain optical coherence tomography (SDOCT), emerged from the clinical realm to provide research scientists with a real-time view of ocular morphology in living animals. We utilized these two independent imaging modalities in a complementary manner to perform in vivo optical sectioning of the adult zebrafish retina. Due to the very high optical power of the zebrafish lens, the confocal depth of field is narrow, allowing for detailed en face views of specific retinal layers, including the cone mosaic. Moreover, we demonstrate that both native reflectance, as well as fluorescent features observed by SLO, can be combined with axial in-depth information obtained by SDOCT. These imaging approaches can be used to screen for ocular phenotypes and monitor retinal pathology in a non-invasive manner.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The adult zebrafish retina: In vivo optical sectioning with Confocal Scanning Laser Ophthalmoscopy and Spectral-Domain Optical Coherence Tomography

Bell

Yuan

DiCicco

et al. 2016

Experimental Eye Research

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“…While contact lenses are commonly used as consumer medical devices for vision correction, they have been increasingly applied in laboratories for in vivo animal retinal imaging and pre-clinical studies 1 2 3 4 5 6 7 . Retinal diseases affect millions of people worldwide, resulting in loss of vision and diminished quality of life.…”

mentioning

confidence: 99%

Fabricating customized hydrogel contact lens

Childs

Lewittes

et al. 2016

Sci Rep

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Contact lenses are increasingly used in laboratories for in vivo animal retinal imaging and pre-clinical studies. The lens shapes often need modification to optimally fit corneas of individual test subjects. However, the choices from commercially available contact lenses are rather limited. Here, we report a flexible method to fabricate customized hydrogel contact lenses. We showed that the fabricated hydrogel is highly transparent, with refractive indices ranging from 1.42 to 1.45 in the spectra range from 400 nm to 800 nm. The Young’s modulus (1.47 MPa) and hydrophobicity (with a sessile drop contact angle of 40.5°) have also been characterized experimentally. Retinal imaging using optical coherence tomography in rats wearing our customized contact lenses has the quality comparable to the control case without the contact lens. Our method could significantly reduce the cost and the lead time for fabricating soft contact lenses with customized shapes, and benefit the laboratorial-used contact lenses in pre-clinical studies.

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An insight on established retinal injury mechanisms and prevalent retinal stem cell activation pathways in vertebrate models

Sherpa

Hui

2021

Anim Models and Exp Med

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The brain processes visual information when light energy transduces into neural activity in the retina. The close-knit components of the central nervous system (CNS), the brain, and its extension retina are thus the critical players in visual perception, thereby aiding in daily activities. While the brain remains well protected inside the skull, the eyes are quite susceptible to physical injuries and chemical accidents. 1 Furthermore, one's genetic makeup and increasing age also invite multiple numbers of eye diseases such as retinitis pigmentosa (RP), age-related macular degeneration (AMD), glaucoma, etc All this has contributed to the recent "World Reports on vision (2019)," which shows that a whopping 2.2 billion people globally fell victim to visual impairment in the past year. 2 The discovery of the existence of adult retinal stem/progenitor cells among different vertebrate species 3 and its high reparative activity in the case of lower vertebrates has presented us with a possibility to "self-heal" the retina one day. 4 Consequently, high regeneration competent animals, which include the amphibian newts and Xenopus, teleost zebrafish (Danio rerio), and chick are thus being explored 5 to investigate different genetic and epigenetic features, signaling pathways, and factors 6,7 that regulate stem cell activation, thus gradually filling in the gaps of our knowledge of mammals, which appear to be the least competent among the group. 8 With the hope of updating and giving researchers an idea about how these animal models have significantly shaped our understanding of the retinal regeneration process, in this review,

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Retinal Regeneration Following OCT-Guided Laser Injury in Zebrafish

Cited by 27 publications

References 16 publications

The adult zebrafish retina: In vivo optical sectioning with Confocal Scanning Laser Ophthalmoscopy and Spectral-Domain Optical Coherence Tomography

The adult zebrafish retina: In vivo optical sectioning with Confocal Scanning Laser Ophthalmoscopy and Spectral-Domain Optical Coherence Tomography

Fabricating customized hydrogel contact lens

An insight on established retinal injury mechanisms and prevalent retinal stem cell activation pathways in vertebrate models

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