Retinoic acid promotes the proliferation of primordial germ cell–like cells differentiated from mouse skin-derived stem cells in vitro

Tan, Hui; Wang, Junjie; Cheng, Shun‐Feng; Ge, Wei; Sun, Ying; Sun, Xiao‐Feng; Sun, Rui; Lan, Li; Li, Bo; Shen, Wei

doi:10.1016/j.theriogenology.2015.09.002

Cited by 27 publications

(42 citation statements)

References 35 publications

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“…Flow cytometric analysis of surface markers CD73 FITC), CD105 (PE) and CD45 (FITC) in mice omentum A) and epididymis B) derived AT-MSCs at cell passage 2 (P2), 4 (P4) and 7 (P7). Since different fat pads have their own metabolic characteristics, fatty acid compositions, and gene expressions (Tchkonia et al, 2002;Schäffler and Büchler, 2007), the source of adipose tissue might be expected to influence AT-MSCS characteristics, such as surface markers and differentiation potential. Therefore, additional studies are necessary to properly understand the molecular characteristics, as well as the plasticity, of the AT-MSCs isolated from different fat depots.…”

Section: Resultsmentioning

confidence: 99%

“…According to the fat pad location, adipose tissue shows different metabolic properties, genes expression profiles, antigenic features, and differentiation potential, influencing AT-MSCs characteristics (Tchkonia et al, 2002).…”

mentioning

confidence: 99%

“…AT-MSCs in vitro differentiation has demonstrated promising results (Zuk et al, 2002) and several substances are used for differentiation of mesenchymal stem cells (MSCs) into diverse cell lineages (Schäffler and Büchler, 2007;Mosna et al, 2010), Including retinoic acid (RA), a vitamin A (retinol)-derivative, which is widely used as a differentiation inducer of MSCs in male germ cells (Nayernia et al, 2006;Huang et al, 2010) Tan et al, 2016. Furthermore, the conditioned medium obtained from the cell culture supernatants is also a strategy applied in differentiation treatments.…”

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confidence: 99%

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The differentiation potential of adipose tissue-derived mesenchymal stem cells into cell lineage related to male germ cells

Bräunig

Glanzner

Rissi

et al. 2018

Arq. Bras. Med. Vet. Zootec.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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The differentiation potential of adipose tissue-derived mesenchymal stem cells into cell lineage related to male germ cells

Bräunig

Glanzner

Rissi

et al. 2018

Arq. Bras. Med. Vet. Zootec.

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“…Several culture systems for viability and proliferation of PGCs have been established up to now. These systems include: adding chicken stem cell factor (chSCF) and fibroblast growth factor 2 (FGF2) to PGC lines [14], addition of retinoic acid (RA) to PGC-like cells differentiated from mouse skin-derived stem cells [15], using steel factor (SLF) and leukaemia inhibitory factor (LIF) [16–19], tumour necrosis factor (TNF)-α [20], RA [21], buffalo rat liver cells (BRL-CM) [22], forskolin (FK), macrophage growth factor (MGF) and kit ligand (KL) [20] in the culture of PGCs derived from epiblast and genital ridge, co-culture (Co-C) of epiblast-derived PGCs with feeder cell monolayer Sertoli cells or SIM mouse embryo-derived thioguanine- and ouabain-resistant (STO) [16,19,23] and using combination of FK, KL and fibroblast feeder cells in culture of human PGCs derived from genital ridge [24–26]. …”

Section: Introductionmentioning

confidence: 99%

“…Morita and Tilly [30] have reported the mitogen activity of RA for germ cells during in vitro foetal mouse oogenesis. Also, the role of RA in inducing PGC formation from 3 to 9 days old mouse embryoid bodies (EBs) [31], skin-derived stem cells [15] and chicken genital ridge [32] was reported.…”

Section: Introductionmentioning

confidence: 99%

Proliferation in culture of primordial germ cells derived from embryonic stem cell: induction by retinoic acid

2016

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An in vitro system that supports primordial germ cells (PGCs) survival and proliferation is useful for enhancement of these cells and efficient transplantation in infertility disorders. One approach is cultivation of PGCs under proper conditions that allow self-renewal and proliferation of PGCs. For this purpose, we compared the effects of different concentrations of retinoic acid (RA), and the effect of PGCs co-culture (Co-C) with SIM mouse embryo-derived thioguanine- and ouabain-resistant (STO) cells on the proliferation of embryonic stem cells (ESCs)-derived PGCs. One-day-old embryoid body (EB) was cultured for 4 days in simple culture system in the presence of 5 ng/ml bone morphogenetic protein-4 (BMP4) (SCB group) for PGC induction. For PGC enrichment, ESCs-derived germ cells were cultured for 7 days in the presence of different doses (0–5 μM) of RA, both in the simple and STO Co-C systems. At the end of the culture period, viability and proliferation rates were assessed and expression of mouse vasa homologue (Mvh), α6 integrin, β1 integrin, stimulated by retinoic acid 8 (Stra8) and piwi (Drosophila)-like 2 (Piwil2) was evaluated using quantitative PCR. Also, the inductive effects were investigated immunocytochemically with Mvh and cadherin1 (CDH1) on the selected groups. Immunocytochemistry/PCR results showed higher expression of Mvh, the PGC-specific marker, in 3 μM RA concentrations on the top of the STO feeder layer. Meanwhile, assessment of the Stra8 mRNA and CDH1 protein, the specific makers for spermatogonia, showed no significant differences between groups. Based on the results, it seems that in the presence of 3 μM RA on top of the STO feeder layer cells, the majority of the cells transdifferentiated into germ cells were PGCs.

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Epidermal growth factor and three‐dimensional scaffolds provide conducive environment for differentiation of mouse embryonic stem cells into oocyte‐like cells

Soleimani

Zareifard

Talaei‐Khozani

et al. 2020

Cell Biology International

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Three‐dimensional (3D) culture provides a biomimicry of the naive microenvironment that can support cell proliferation, differentiation, and regeneration. Some growth factors, such as epidermal growth factor (EGF), facilitate normal meiosis during oocyte maturation in vivo. In this study, a scaffold‐based 3D coculture system using purified alginate was applied to induce oocyte differentiation from mouse embryonic stem cells (mESCs). mESCs were induced to differentiate into oocyte‐like cells using embryoid body protocol in the two‐dimensional or 3D microenvironment in vitro. To increase the efficiency of the oocyte‐like cell differentiation from mESCs, we employed a coculture system using ovarian granulosa cells in the presence or absence of epidermal growth factor (+EGF or −EGF) for 14 days and then the cells were assessed for germ cell differentiation, meiotic progression, and oocyte maturation markers. The cultures exposed to EGF in the alginate‐based 3D microenvironment showed the highest level of premeiotic (Oct4 and Mvh), meiotic (Scp1, Scp3, Stra8, and Rec8), and oocyte maturation (Gdf9, Cx37, and Zp2) marker genes (p < .05) in comparison to other groups. According to the gene‐expression patterns, we can conclude that alginate‐based 3D coculture system provided a highly efficient protocol for oocyte‐like cell differentiation from mESCs. The data showed that this culture system along with EGF improved the rate of in vitro oocyte‐like cell differentiation.

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Retinoic acid promotes the proliferation of primordial germ cell–like cells differentiated from mouse skin-derived stem cells in vitro

Cited by 27 publications

References 35 publications

The differentiation potential of adipose tissue-derived mesenchymal stem cells into cell lineage related to male germ cells

The differentiation potential of adipose tissue-derived mesenchymal stem cells into cell lineage related to male germ cells

Proliferation in culture of primordial germ cells derived from embryonic stem cell: induction by retinoic acid

Epidermal growth factor and three‐dimensional scaffolds provide conducive environment for differentiation of mouse embryonic stem cells into oocyte‐like cells

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