RETRACTED ARTICLE: Dwarf mutations in grass pea (Lathyrus sativus L.): origin, morphology, inheritance and linkage studies

Abstract

Induction of mutation has been used to create additional genetic variability in grass pea (Lathyrus sativus L.). During the ongoing investigations on different induced-morphological mutants, the author detected three types of dwarf mutants in grass pea. One mutant, designated as dwf1 type was earlier identified in colchicine-induced C2 generation of grass pea variety BioR-231 while the other two, designated as dwf2 and dwf3 were isolated in 250 Gy and 300 Gy gamma ray irradiated M2 progeny of variety 'BioR-231' and 'Hooghly Local', respectively. As compared to their parental varieties (controls), all the three mutants manifested stunted, erect and determinate stem, early maturity and tolerance to pod shattering habit. The mutants differed from each other, as well as with controls, in number of primary branches, nature of stipules and internodes, length of peduncle, leaflet and seed coat colour, seed yield and seed neurotoxin content. The three dwarf mutants were monogenically recessive and bred true in successive generations. F2 segregation pattern obtained from the crosses involving the three mutants indicated that dwarf mutation in grass pea was controlled by two independent non-allelic genes, assigned as df1 (for dwf1 type), df2 (for dwf2 type) and df3 (for dwf3 type), with the df1 locus being multiple allelic. Primary trisomic analyses revealed the presence of df1/df2 locus on the extra chromosome of trisomic type I, whereas df3 was located on the extra chromosome of type III. Linkage studies involving five other phenotypic markers suggested linked association of df1/df2 locus with lfc (leaflet colour) and wgn (winged internode) and df3 locus with cbl (seed coat colour). Both the loci; however, assorted independently with flower colour and stipule character. The dwarf types can be utilized as valuable tools for further cytogenetic research and breeding of grass pea.

Mutagenic Effectiveness and Efficiency of Gamma Rays and HZ with Phenotyping of Induced Mutations in Lentil Cultivars

“…It was observed that the progenies of tall, dwarf, bushy, prostrate and bold seeded mutants bred true for the altered traits in M 3 generation. The genes responsible for the altered plant size and growth habits were reported to be monogenic recessive [32][33][34][35][36][37]. While Konzak et al [38] and Shakoor et al [39] in wheat and triticale, respectively, reported that the polygenes are responsible for semi dwarf character in mutants; Qin et al [40] reported a dominant dwarf mutation in the single gene in rice.…”

Differential Response of Cysteine-deficient Lentil (<i>Lens culinaris</i> Medik.) Mutants Impaired in Foliar O-acetylserine(thiol)-lyase Expression

“…Growth retardation is a common phenomenon orchestrated through mutagenesis, as also observed in other legumes (Talukdar, 2009;Fazal Ali et al, 2010;Kozgar et al, 2012). In lentil, similar phenomenon was observed in EMS-induced two mutant lines catLc1 and catLc2, impaired in catalase activities, but the mutants differed in magnitude of growth retardation of shoots and roots (Talukdar and Talukdar, 2013a).…”

“…This technique has been successfully used to develop cytogenetic and breeding tools in different legume crops including lentil (Fazal Ali et al, 2010;Talukdar, 2009;2013), the potential of which is now being exploited to ascertain the intrinsic metabolic events in grain legumes (Talukdar, 2012a;2012b;Tsyganov et al, 2013). Lentil is a cool-season edible pulse crop grown widely in the Indian subcontinent, West Asia, North Africa and parts of Europe, Oceania and North America (Erskine et al, 2011) and has tremendous health benefits (Erskine et al, 2011;Talukdar, 2012c).…”

Cytogenetics of a reciprocal translocation integrating distichous pedicel and tendril-less leaf mutations inLathyrus sativusL.

“…It can be used as an excellent cytogenetic tool along with other different stocks, developed in this crop for gene mapping and linkage group analysis (Talukdar 2008b(Talukdar , 2009a(Talukdar ,b,c, 2012a(Talukdar , 2012c, and also to reveal antioxidant defense response at aneuploid levels (Talukdar 2011c). Occurrence of trisomics in the progeny of translocation heterozygote was also reported in different legume crops (Sutton 1939;Pellew 1940;Müller 1975;Mercykutty and Kumar 1985).…”