RETRACTED ARTICLE: Long noncoding RNA SNHG15 enhances the development of colorectal carcinoma via functioning as a ceRNA through miR-141/SIRT1/Wnt/β-catenin axis

Abstract

Colon cancer, also known as colorectal carcinoma (CRC), remains to be one of the most mainsprings of cancer-produced deaths entire world. We planned to grab the role and possible biological cause of a long noncoding RNA, namely, small nucleolar RNA host gene 15 (SNHG15), in CRC. The mRNA level of SNHG15 in CRC tissues and cells was detected, followed by investigating the impacts of the depression of SNHG15 on CRC cell proliferation (viability and colony-forming), apoptosis, migration, and invasion. Moreover, the association between SNHG15 and miR-141 and the correlation between miR-141 and SIRT1 were also explored. Besides, the influences of dysregulated SNHG15 on the Wnt/b-catenin signalrelated proteins were determined. SNHG15 was highly expressed in CRC tissues and cells. Depression of SNHG15 depressed proliferation, enhanced apoptosis, and repressed the migration and invasion of CRC cells. In addition, SNHG15 presented a downside tendency on regulating miR-141, and the miR-141 inhibitor dramatically changeover the impacts of SNHG15 depression on tumor growth and metastasis. Moreover, SIRT1 was verified as a functional target of miR-141 in CRC cells. Besides, the suppression of SNHG15 remarkably controlled activating the Wnt/b-catenin signals, which was reversed after inhibiting miR-141 at the same time. The investigated results in this research revealed that the increased expression of SNHG15 may enhance the process of CRC by acting as a ceRNA in regulating SIRT1 expression by sponging miR-141. Thus we propose that Wnt/b-catenin signals may be a downriver regulator in mediating the impacts of SNHG15 in CRC and SNHG15-miR-141-SIRT1 axis may pave a new sight in explaining the biological processes of CRC.

An Emerging Class of Long Non-coding RNA With Oncogenic Role Arises From the snoRNA Host Genes

“…SNHG15 is involved in colorectal cancer cell proliferation and migration via miR-141 (159) and miR-338-3p sponging (160), along with up-regulation of AIF (Allograft Inflammatory Factor 1) (152), and activation of transcription factor SLUG (45). In thyroid cancer, SNHG15 is a ceRNA for miR-510-5p (161) and miR-200a-3p (162).…”

LncRNA SNHG15: A new budding star in human cancers

“…Both univariate and multivariate analysis confirmed that SNHG15 expression was significantly associated with overall survival (OS) in CRC patients, suggesting that SNHG15 could be used as an independent prognostic factor and potential biomarker for CRC patients . Functional assays further demonstrated that knockdown of SNHG15 could inhibit CRC cell proliferation, activate cell apoptosis and suppress cell migration and invasion both in vitro and in vivo . Recently, SNHG15 has been shown to present a downside tendency on regulating miR‐141, which downregulates sirtuin 1 (SIRT1) in CRC cells.…”

“…LncRNA SNHG15 has been found in several studies to be dramatically upregulated in CRC tissues and cells . CRC patients with larger tumour size, advanced TNM stage and lymph node metastasis show higher SNHG15 expression . Furthermore, higher SNHG15 expression is correlated with a worse survival .…”

Molecular therapies delaying cardiovascular aging: disease- or health-oriented approaches

“…Several studies have shown the interaction of miR-141 with lncRNAs in different diseases including cancers (80,81,82). However, only few studies are available on lncRNAs and circRNAs that sponge miR-141 in CVD.…”