RETRACTED: Knockdown of ANRIL aggravates H2O2-induced injury in PC-12 cells by targeting microRNA-125a

Li, Ran; Yin, Fei; Guo, Yingying; Zhao, Kunchi; Ruan, Qing; Qi, Ying-Mei

doi:10.1016/j.biopha.2017.05.122

Cited by 58 publications

(42 citation statements)

References 33 publications

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“…9 In addition, miR-125a low expression is previously reported to induce neutrophil recruitment and regulate lung inflammation in SLE. 12 Known that miR-125a is a target of lncRNA ANRIL, [14][15][16][17][18] and both lncRNA ANRIL and miR-125a regulate inflammatory responses including lung inflammation, we hypothesized that lncRNA ANRIL might interact with miR-125a, and lncRNA ANRIL/miR-125a axis might play critical roles in bronchial asthma as well. In our analysis, we discovered that lncRNA ANRIL/miR-125a axis was of good predictive value for the risk of disease exacerbation in bronchial asthma as well as in distinguishing bronchial asthma patients from control.…”

Section: Ta B L Ementioning

confidence: 99%

“…In addition, decreased level of miR‐125a is associated with lung inflammation and acute alveolar hemorrhage in SLE patients by recruiting of neutrophils . Based on the existing evidence, lncRNA ANRIL/miR‐125a axis is reported to participate in oxidative damage during spinal cord injury, and it also facilitates carcinogenesis in several cancers . Considering that lncRNA ANRIL and miR‐125a are closely correlated with inflammatory diseases including lung inflammation, we speculated that lncRNA ANRIL/miR‐125a axis might participate in etiology of bronchial asthma as well.…”

Section: Introductionmentioning

confidence: 96%

“…12 Based on the existing evidence, lncRNA ANRIL/miR-125a axis is reported to participate in oxidative damage during spinal cord injury, and it also facilitates carcinogenesis in several cancers. [14][15][16][17][18] Considering that lncRNA ANRIL and miR-125a are closely correlated with inflammatory diseases including lung inflammation, we speculated that lncRNA ANRIL/miR-125a axis might participate in etiology of bronchial asthma as well. Nevertheless, limited study has been reported.…”

Section: Introductionmentioning

confidence: 99%

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LncRNA ANRIL/miR‐125a axis exhibits potential as a biomarker for disease exacerbation, severity, and inflammation in bronchial asthma

Zhu

Feng

2019

Clinical Laboratory Analysis

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Background This study aimed to explore the correlation of lncRNA ANRIL/miR‐125a axis with disease risk, severity, and inflammatory cytokines of bronchial asthma. Methods Plasma samples from 90 patients with bronchial asthma at exacerbation (BA‐E), 90 with bronchial asthma at remission (BA‐R), and 90 controls (healthy subjects) were collected. The qPCR was used for lncRNA ANRIL and miR‐125a detection, and ELISA was adopted for pro‐inflammatory cytokines detection. Participants’ characteristics, laboratory tests, and the pulmonary ventilation function examinations were recorded. Results LncRNA ANRIL was negatively correlated with miR‐125a in BA‐E patients, BA‐R patients, and controls. LncRNA ANRIL/miR‐125a axis was upregulated in BA‐E patients compared with BA‐R patients and controls. ROC curve analyses illuminated that lncRNA ANRIL/miR‐125a axis was of good value in distinguishing BA‐E patients from BA‐R patients and controls. As to pulmonary ventilation functions, lncRNA ANRIL/miR‐125a axis was negatively associated with FEV1/FVC and FEV1%predicted in bronchial asthma patients, especially in BA‐E patients. Regarding inflammation, lncRNA ANRIL/miR‐125a axis was positively correlated with pro‐inflammatory cytokines in bronchial asthma patients, especially in BA‐E patients. In addition, lncRNA ANRIL/miR‐125a axis was positively correlated with exacerbation severity in BA‐E patients. Conclusion LncRNA ANRIL/miR‐125a is potentially indicative of disease exacerbation, exacerbation severity, and inflammation for bronchial asthma, while these findings are preliminary and need further confirmation.

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Section: Ta B L Ementioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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LncRNA ANRIL/miR‐125a axis exhibits potential as a biomarker for disease exacerbation, severity, and inflammation in bronchial asthma

Zhu

Feng

2019

Clinical Laboratory Analysis

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“…miR‐125a, a highly reserved miRNA locating on chromosome 19, 11, and 21, is also closely related to inflammation and immunity, and this miRNA has been reported to have the potential in regulating sepsis pathogenesis . Moreover, it is a direct target of lnc‐ANRIL, and the lnc‐ANRIL/miR‐125a axis has been indicated in many diseases . However, the role of lnc‐ANRIL/miR‐125a axis in sepsis remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Elevated circulating lnc‐ANRIL/miR‐125a axis level predicts higher risk, more severe disease condition, and worse prognosis of sepsis

Gui

Peng

Liu

2019

Clinical Laboratory Analysis

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Aim This study aimed to investigate the correlation of lnc‐ANRIL/miR‐125a axis with risk, severity, inflammation, and prognosis of sepsis. Methods A hundred and twenty‐six sepsis patients and 125 healthy controls were recruited, and then, blood samples were collected, and plasma was separated for lnc‐ANRIL, miR‐125a, lnc‐ANRIL/miR‐125a axis, and inflammatory cytokine level detections. In addition, basic characteristics, 28‐day mortality, and accumulating survival of sepsis patients were recorded. Results Plasma lnc‐ANRIL expression was increased, miR‐125a expression was decreased, and lnc‐ANRIL/miR‐125a axis level was elevated in sepsis patients compared with healthy controls, and all of them had good value for predicting sepsis risk with AUCs of 0.800, 0.817, and 0.843, respectively. Lnc‐ANRIL and lnc‐ANRIL/miR‐125a axis were positively correlated with biochemical index levels including CRP and PCT levels, disease severity scale scores, and pro‐inflammatory cytokine levels in sepsis patients, while miR‐125a displayed the opposite trend. Lnc‐ANRIL and lnc‐ANRIL/miR‐125a axis expressions were elevated, while miR‐125a expression was declined in deaths compared with survivors, and all of them predicted 28‐day mortality in sepsis patients with AUCs of 0.765, 0.745, and 0.785, respectively. Subsequently, the Kaplan‐Meier analysis revealed that patients with high lnc‐ANRIL, low miR‐125a, and high lnc‐ANRIL/miR‐125a axis levels presented with worse accumulating survival. In addition, multivariate regression model analyses revealed that high plasma lnc‐ANRIL/miR‐125a axis was an independent predictive factor for both increased 28‐day mortality and worse accumulating survival. Conclusion Circulating lnc‐ANRIL/miR‐125a axis was upregulated and could serve as a biomarker for severity, inflammation, and prognosis in sepsis patients.

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“…Then, the proteins were quantified using Pierce TM BCA Protein Assay kit (Thermo Fisher Scientific, Waltham, MA, USA). Western blotting system was established as previously described [21]. Equal concentrations of proteins were electrophoresed in polyacrylamide gels and transferred onto nitrocellulose (NC) membranes (Millipore, Bedford, MA, USA).…”

Section: Western Blottingmentioning

confidence: 99%

Long Non-Coding RNA Urothelial Carcinoma Associated 1 Promotes Proliferation, Migration and Invasion of Osteosarcoma Cells by Regulating microRNA-182

Xing

Gong

et al. 2018

Cell Physiol Biochem

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Background/Aims: Previous studies demonstrated the oncogenic roles of lncRNA UCA1 in osteosarcoma. This study aimed to explore the internal molecular mechanism of UCA1 on promoting osteosarcoma cell proliferation, migration and invasion. Methods: qRT-PCR was conducted to measure the expression levels of UCA1, miR-182 and TIMP2. Cell transfection was used to change the expression levels of UCA1, miR-182 and TIMP2. Cell viability, migration, invasion and apoptosis were measured using CCK-8 assay, two-chamber migration (invasion) assay and Guava Nexin assay, respectively. The associations between UCA1, miR-182 and iASPP were analyzed by dual luciferase activity assay. The protein expression levels of key factors involved in cell apoptosis, PI3K/AKT/GSK3β pathway and NF-κB pathway, as well as p53, Rb, RECQ family and iASPP were evaluated by western blotting. Results: UCA1 was highly expressed in osteosarcoma MG63 and OS-732 cells. Knockdown of UCA1 inhibited OS-732 cell viability, migration and invasion, but promoted cell apoptosis. miR-182 was up-regulated in OS-732 cells after UCA1 knockdown and participated in the effects of UCA1 on OS-732 cells. TIMP2 was downstream factor of miR-182 and involved in the regulatory roles of miR-182 on OS-732 cell viability, migration, invasion, apoptosis, as well as PI3K/AKT/GSK3β and NF-κB pathways. UCA1 knockdown up-regulated p53, Rb and RECQL5 levels in OS-732 cells, while down-regulated the expression of iASPP. TGF-β or TNF-α treatment could enhance the expression of UCA1 in OS-732 cells. Conclusion: Our research verified that UCA1 exerted oncogenic roles in osteosarcoma cells by regulating miR-182 and TIMP2, as well as PI3K/AKT/GSK3β and NF-κB pathways.

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RETRACTED: Knockdown of ANRIL aggravates H2O2-induced injury in PC-12 cells by targeting microRNA-125a

Cited by 58 publications

References 33 publications

LncRNA ANRIL/miR‐125a axis exhibits potential as a biomarker for disease exacerbation, severity, and inflammation in bronchial asthma

LncRNA ANRIL/miR‐125a axis exhibits potential as a biomarker for disease exacerbation, severity, and inflammation in bronchial asthma

Elevated circulating lnc‐ANRIL/miR‐125a axis level predicts higher risk, more severe disease condition, and worse prognosis of sepsis

Long Non-Coding RNA Urothelial Carcinoma Associated 1 Promotes Proliferation, Migration and Invasion of Osteosarcoma Cells by Regulating microRNA-182

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