RETRACTED: LINC02381 aggravates breast cancer through the miR‐1271‐5p/FN1 axis to activate PI3K/AKT pathway

Huang, Ping; Wu, Huazhang; Wang, Song; Liu, Guodong

doi:10.1002/mc.23375

Cited by 26 publications

(18 citation statements)

References 39 publications

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“…FN1 encodes fibronectin, an ECM glycoprotein involved in cell adhesion and migration. FN1 plays a critical role in the development of ovarian ( Liang et al, 2020 ), thyroid ( Geng et al, 2021 ), renal ( Waalkes et al, 2010 ), cervical ( Zhou et al, 2019a ), breast ( Huang et al, 2022 ), and gastric cancers ( Han et al, 2020 ). Liu et al (2020) reported that the expression level of FN1 is also correlated with a poor HNSCC prognosis and that p62/SQSTM1 may participate as an autophagy adapter in the autophagy-lysosome pathway for FN1 degradation.…”

Section: Discussionmentioning

confidence: 99%

Novel prognostic matrisome-related gene signature of head and neck squamous cell carcinoma

Huang

Liang

Dong

et al. 2022

Front. Cell Dev. Biol.

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Background: Head and neck squamous cell carcinoma (HNSCC) is a common malignancy of the mucosal epithelium of the oral cavity, pharynx, and larynx. Laryngeal squamous cell carcinoma (LSCC) and oral squamous cell carcinoma are common HNSCC subtypes. Patients with metastatic HNSCC have a poor prognosis. Therefore, identifying molecular markers for the development and progression of HNSCC is essential for improving early diagnosis and predicting patient outcomes.Methods: Gene expression RNA-Seq data and patient clinical traits were obtained from The Cancer Genome Atlas-Head and Neck Squamous Cell Carcinoma (TCGA-HNSC) and Gene Expression Omnibus databases. Differentially expressed gene (DEG) screening was performed using the TCGA-HNSC dataset. Intersection analysis between the DEGs and a list of core matrisome genes obtained from the Matrisome Project was used to identify differentially expressed matrisome genes. A prognostic model was established using univariate and multivariate Cox regression analyses, least absolute shrinkage, and selection operator (LASSO) regression analysis. Immune landscape analysis was performed based on the single-sample gene set enrichment analysis algorithm, Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, prognostic value, receiver operating characteristic curve analysis, and gene mutation analyses. Immunohistochemical results regarding prognostic protein levels were obtained from the Human Protein Atlas. Single-gene RNA-sequencing data were obtained from GSE150321 and GSE172577 datasets. CCK-8 and Transwell assays were used to confirm cell proliferation and migration.Results: A total of 1,779 DEGs, including 939 upregulated and 840 downregulated genes, between tumor and normal samples were identified using the TCGA-HNSC microarray data. Intersection analysis revealed 52 differentially expressed matrisome-related genes. After performing univariate and multivariate Cox regression and LASSO analyses, a novel prognostic model based on six matrisome genes (FN1, LAMB4, LAMB3, DMP1, CHAD, and MMRN1) for HNSCC was established. This risk model can successfully predict HNSCC survival. The high-risk group had worse prognoses and higher enrichment of pathways related to cancer development than the low-risk group. Silencing LAMB4 in HNSCC cell lines promoted cell proliferation and migration.Conclusion: This study provides a novel prognostic model for HNSCC. Thus, FN1, LAMB4, LAMB3, DMP1, CHAD, and MMRN1 may be the promising biomarkers for clinical practice.

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Section: Discussionmentioning

confidence: 99%

Novel prognostic matrisome-related gene signature of head and neck squamous cell carcinoma

Huang

Liang

Dong

et al. 2022

Front. Cell Dev. Biol.

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“…In cancer-related research, miR-1271-5p is considered to be a tumor suppressor. MiR-1271-5p had been verified to decrease proliferation and metastasis in breast cancer [23] and glioma [24]. Also, studies had indicated that miR-1271-5p enhanced the cisplatin sensitivity of ovarian cancer by inhibiting cell growth and metastasis [25].…”

Section: Discussionmentioning

confidence: 99%

Circ_0039569 contributes to the paclitaxel resistance of endometrial cancer via targeting miR-1271-5p/PHF6 pathway

Zhang

et al. 2022

Anti-Cancer Drugs

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Circular RNA (circRNA) has been confirmed to be involved in the chemoresistance process of cancers. However, whether circ_0039569 mediates the chemoresistance of endometrial cancer (EC) remains unclear. Quantitative real-time PCR was performed to analyze circ_0039569, microRNA (miR)-1271-5p and PHD finger protein 6 (PHF6) expression. Cell counting kit-8 assay was used to assess the paclitaxel (PTX) resistance of cells. Cell proliferation, apoptosis and invasion were determined using EdU assay, colony formation assay, flow cytometry and transwell assay. Protein expression was examined by western blot analysis. RNA interaction was verified by dual-luciferase reporter assay and RNA pull-down assay. Xenograft tumor models were constructed to explore the effect of circ_0039569 knockdown on the PTX sensitivity of EC tumors. Circ_0039569 was upregulated in PTX-resistant EC tissues and cells. Knockdown of circ_0039569 enhanced the PTX sensitivity of EC cells by inhibiting cell growth and invasion. MiR-1271-5p could be sponged by circ_0039569, and its inhibitor abolished the regulation of circ_0039569 knockdown on the PTX sensitivity of EC cells. PHF6 was targeted by miR-1271-5p, and its overexpression eliminated the promotion effect of miR-1271-5p on the PTX sensitivity of EC cells. Also, interference of circ_0036569 enhanced the PTX sensitivity of EC tumors by regulating the miR-1271-5p/PHF6 pathway. Collectively, circ_0039569 might contribute to the PTX resistance of EC through the regulation of the miR-1271-5p/PHF6 axis.

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“…Survival analysis revealed that patients in the high-risk group had a much worse prognosis. Moreover, patients with high expression levels of seven GT-related lncRNAs turned cancer cells, including cell proliferation, migration, and invasion (45). Moreover, LINC02381 plays an oncogenic role in cervical cancer and osteosarcoma (46,47).…”

Section: Discussionmentioning

confidence: 99%

“…Huang et al. found that LINC02381 was upregulated in breast cancer, and knockdown of LINC02381 impaired the malignant phenotypes of breast cancer cells, including cell proliferation, migration, and invasion ( 45 ). Moreover, LINC02381 plays an oncogenic role in cervical cancer and osteosarcoma ( 46 , 47 ).…”

Section: Discussionmentioning

confidence: 99%

Glycosyltransferase-related long non-coding RNA signature predicts the prognosis of colon adenocarcinoma

Zhang

et al. 2022

Front. Oncol.

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PurposeColon adenocarcinoma (COAD) is the most common type of colorectal cancer (CRC) and is associated with poor prognosis. Emerging evidence has demonstrated that glycosylation by long noncoding RNAs (lncRNAs) was associated with COAD progression. To date, however, the prognostic values of glycosyltransferase (GT)-related lncRNAs in COAD are still largely unknown.MethodsWe obtained the expression matrix of mRNAs and lncRNAs in COAD from The Cancer Genome Atlas (TCGA) database. Then, the univariate Cox regression analysis was conducted to identify 33 prognostic GT-related lncRNAs. Subsequently, LASSO and multivariate Cox regression analysis were performed, and 7 of 33 GT-related lncRNAs were selected to conduct a risk model. Gene set enrichment analysis (GSEA) was used to analyze gene signaling pathway enrichment of the risk model. ImmuCellAI, an online tool for estimating the abundance of immune cells, and correlation analysis were used to explore the tumor-infiltrating immune cells in COAD. Finally, the expression levels of seven lncRNAs were detected in colorectal cancer cell lines by reverse transcription-quantitative polymerase chain reaction (RT-qPCR).ResultsA total of 1,140 GT-related lncRNAs were identified, and 7 COAD-specific GT-related lncRNAs (LINC02381, MIR210HG, AC009237.14, AC105219.1, ZEB1-AS1, AC002310.1, and AC020558.2) were selected to conduct a risk model. Patients were divided into high- and low-risk groups based on the median of risk score. The prognosis of the high-risk group was worse than that of the low-risk group, indicating the good reliability and specificity of our risk model. Additionally, a nomogram based on the risk score and clinical traits was built to help clinical decisions. GSEA showed that the risk model was significantly enriched in metabolism-related pathways. Immune infiltration analysis revealed that five types of immune cells were significantly different between groups, and two types of immune cells were negatively correlated with the risk score. Besides, we found that the expression levels of these seven lncRNAs in tumor cells were significantly higher than those in normal cells, which verified the feasibility of the risk model.ConclusionThe efficient risk model based on seven GT-related lncRNAs has prognostic potential for COAD, which may be novel biomarkers and therapeutic targets for COAD patients.

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RETRACTED: LINC02381 aggravates breast cancer through the miR‐1271‐5p/FN1 axis to activate PI3K/AKT pathway

Cited by 26 publications

References 39 publications

Novel prognostic matrisome-related gene signature of head and neck squamous cell carcinoma

Novel prognostic matrisome-related gene signature of head and neck squamous cell carcinoma

Circ_0039569 contributes to the paclitaxel resistance of endometrial cancer via targeting miR-1271-5p/PHF6 pathway

Glycosyltransferase-related long non-coding RNA signature predicts the prognosis of colon adenocarcinoma

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