RETRACTED: The myth of plant transformation

Rao, Abdul Qayyum; Bakhsh, Allah; Kiani, Sarfraz; Shahzad, Kamran; Shahid, Ahmad Ali; Husnain, Tayyab; Riazuddin, S.

doi:10.1016/j.biotechadv.2019.06.003

Cited by 14 publications

(16 citation statements)

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“…The successful establishment of HRs has been reported in many plant species. However, the molecular mechanism of HRs is still unknown, but it is clear that the transfer of T‐DNA containing the rol gene from the Agrobacterium Ri plasmid into the host genome plays a major role in the formation of HRs and the accumulation of biochemical compounds . Depending on the Agrobacterium strain, the plasmid triggers the synthesis of various opines, including agropine, mannopine, cucumopine, and mikimopine .…”

Section: Discussionmentioning

confidence: 99%

Agrobacterium rhizogenes mediated transformation of Ficus carica L. for the efficient production of secondary metabolites

et al. 2020

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BACKGROUND Ficus carica L., an ancient source of food and medicines, is rich in valuable nutritional and secondary compounds with antioxidant, antimicrobial, and anticancer effects. The present study is the first attempt to examine hairy root (HR) induction of F. carica (Sabz and Siah) by inoculating the 3‐week‐old shoots and leaves with different strains of Agrobacterium rhizogenes and also to investigate methyl jasmonate (MeJA) elicitation of HRs to produce a fast and high‐yield production method for secondary metabolites. RESULTS The maximum transformation rate (100%) was achieved by inoculating the shoots with Agrobacterium rhizogenes strain A7. Siah HRs elicited with 100 and 200 μmol L−1 MeJA and Sabz HRs with 100 μmol L−1 MeJA showed the highest total phenolic content. The highest flavonoid content was 3.935 mg QE g−1 DW in Siah HRs treated with 200 μmol L−1 MeJA and 2.762 mg QE g−1 DW in Sabz HRs treated with 300 μmol L−1 MeJA. The 2, 2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging capacity and ferric reducing antioxidant power (FRAP) value of HRs were affected by MeJA treatments. Methyl jasmonate elicitation also significantly enhanced the content of six phenolic acids (gallic acid, caffeic acid, chlorogenic acid, coumaric acid, rosmarinic acid, and cinnamic acid) and three flavonoids (rutin, quercetin, and apigenin). Thymol, a monoterpene phenol, was the main HR compound detected in gas chromatography mass spectrometry (GC–MS) analysis of the essential oils. CONCLUSION Induction of HRs and elicitation of F. carica HRs by MeJA resulted in a significant increase in the production of important phenolic compounds and a significant increase in antioxidant capacity. © 2020 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Agrobacterium rhizogenes mediated transformation of Ficus carica L. for the efficient production of secondary metabolites

et al. 2020

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“…Genetic transformation can be achieved by physical or biological methods. Physical methods are usually used to transfer non-viral vectors by using a gene gun (biolistics) and chemical polyethylene glycol (PEG) transformation or electroporation of protoplasts (Barampuram and Zhang, 2011;Rao et al, 2009). The biological method of plant cell genetic transformation by Agrobacterium involves the transfer and integration of a large tumor-inducing (Ti) or rhizogenic (Ri) plasmid resident in Agrobacterium into the plant nuclear genome.…”

Section: Transformation Methods and Expression Systemsmentioning

confidence: 99%

“…The biological method of plant cell genetic transformation by Agrobacterium involves the transfer and integration of a large tumor-inducing (Ti) or rhizogenic (Ri) plasmid resident in Agrobacterium into the plant nuclear genome. Although several species of Agrobacterium are known, two species are predominantly used for transformation: Agrobacterium tumefaciens (T-DNA transfer for generation of transgenic plants) and Agrobacterium rhizogenes (Ri-plasmid transfers necessary DNA to generate transgenic hairy roots) (Gelvin, 2003;Gleba et al, 2014;Rao et al, 2009).…”

Section: Transformation Methods and Expression Systemsmentioning

confidence: 99%

Plant-based expression systems for protein and antimicrobial peptide production

Niknejad

2018

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Molecular farming technology offers a unique advantage that almost any protein can be produced economically and safely under very controlled conditions. Besides traditional production systems, such as bacteria, yeasts, insects and mammal cell lines, plants can now be used to produce eukaryotic recombinant proteins, especially therapeutic ones. Their advantages as hosts for protein production include correct post-translational modifications, lowcost maintenance and no risk of contamination by human pathogens. The system is widely applied in agriculture and industry, especially in life science and pharmaceutical industry. The application of transgenic plants in the production of vaccines, antibodies and pharmaceutical proteins has been playing a key role in plant genetic engineering in recent years. The production of recombinant proteins plays a critical role in the production of high amounts of high-quality proteins. In this review, common problems in the production of recombinant proteins and antimicrobial peptides in plant-based expression systems are discussed and strategies for their solution are suggested. Viral vector-mediated transient gene expression in plants enables rapid production of pharmaceutical proteins such as vaccine antigens and antibodies. To conclude, plant-based systems have the potential to bring unique efficacy-enhancing features to increase the utility and effectiveness of vaccines and therapeutics.

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“…Dies kann bei Pflanzen über verschiedene Verfahren erreicht werden, unter anderem vermittelt über das Bodenbakterium Agrobacterium tumefaciens und dessen sogenannter T‐DNA. In diesem Mechanismus überträgt das Bakterium natürlicherweise einen spezifischen Abschnitt extrachromosomaler DNA in das Pflanzengenom, der im Labor durch jegliche gewünschte DNA ausgetauscht werden kann .…”

Section: Klassische Gentechnikunclassified

Das CRISPR/Cas‐System

Schindele¹,

Wolter²,

Puchta

2018

Biologie in unserer Zeit

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Zusammenfassung Die Pflanzenzucht nutzte bisher ungezielte und zeitintensive Verfahren, um Verbesserungen in den agronomischen Eigenschaften unserer Kulturpflanzen zu erreichen. Dies hat sich in den vergangenen Jahren grundlegend geändert, denn mit dem CRISPR/Cas‐System steht nun ein hochpräzises und zuverlässiges Werkzeug zur Verfügung, um diese Ziele schnell und effizient zu erreichen. Durch die programmierbare Induktion von Doppelstrangbrüchen können gezielt an fast jeder beliebigen Stelle im Genom die gewünschten Veränderungen vorgenommen werden, die sich nicht mehr von natürlichen Veränderungen unterscheiden lassen. So entstanden bereits eine Reihe von Kulturpflanzen mit agronomisch interessanten Eigenschaften. Die momentane Charakterisierung weiterer CRISPR/Cas‐Systeme erweitert nicht nur unseren molekularen Werkzeugkasten, sondern beginnt die gesamte Biotechnologie und Biologie grundlegend zu verändern.

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RETRACTED: The myth of plant transformation

Cited by 14 publications

References 0 publications

Agrobacterium rhizogenes mediated transformation of Ficus carica L. for the efficient production of secondary metabolites

Agrobacterium rhizogenes mediated transformation of Ficus carica L. for the efficient production of secondary metabolites

Plant-based expression systems for protein and antimicrobial peptide production

Das CRISPR/Cas‐System

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